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Impact of different pH thresholds for 24-hour dual probe pH monitoring in patients with suspected laryngopharyngeal reflux

  • O Reichel (a1) and W J Issing (a2)
  • DOI:
  • Published online: 01 May 2008

The gold standard test for laryngopharyngeal reflux is 24-hour pH monitoring, which determines the reflux area index with a pH threshold of less than four (i.e. the reflux area index four). However, refluxed pepsin is able to cause laryngeal injury at pH levels above five.

Study design:

Prospective study.

Materials and methods:

In order to establish normative values for a reflux area index with a pH threshold of less than five (i.e. the reflux area index five), 29 healthy volunteers underwent pH monitoring. In 45 patients with suspected laryngopharyngeal reflux, reflux area index four and reflux area index five were determined by pH study.


In healthy volunteers, the reflux area index five was 72.6 (95th percentile). In 29 of 44 patients, laryngopharyngeal reflux was diagnosed due to a reflux area index four of greater than 6.3. However, the reflux area index five revealed laryngopharyngeal reflux in six more patients.


For exact analysis of pH monitoring results, two pH thresholds (less than four and less than five) must be considered. Further studies with a larger number of healthy volunteers are necessary in order to reveal normative values for the reflux area index five parameter.

Corresponding author
Address for correspondence: Dr Oliver Reichel, Department of Otorhinolaryngology, Head and Neck Surgery, Ludwig Maximilians University Munich, Klinikum Grosshadern, Marchionini-Strasse 15, 81377 Munich, Germany. Fax: +49 89 70 95 68 69 E-mail:
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