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Protective effects of vitamins E, B and C and l-carnitine in the prevention of cisplatin-induced ototoxicity in rats

Published online by Cambridge University Press:  11 April 2012

S Alıcura Tokgöz ,
E Vuralkan ,
N D Sonbay ,
M Çalişkan ,
C Saka ,
Ö Beşalti  and
İ Akin
S Alıcura Tokgöz*
Affiliation:
First ENT Clinic, Ministry of Health Dışkapı Yıldırım Beyazıt Training and Research Hospital, Ankara, Turkey
E Vuralkan
Affiliation:
First ENT Clinic, Ministry of Health Dışkapı Yıldırım Beyazıt Training and Research Hospital, Ankara, Turkey
N D Sonbay
Affiliation:
First ENT Clinic, Ministry of Health Dışkapı Yıldırım Beyazıt Training and Research Hospital, Ankara, Turkey
M Çalişkan
Affiliation:
Faculty of Veterinary Medicine, Ankara University, Turkey
C Saka
Affiliation:
First ENT Clinic, Ministry of Health Dışkapı Yıldırım Beyazıt Training and Research Hospital, Ankara, Turkey
Ö Beşalti
Affiliation:
Faculty of Veterinary Medicine, Ankara University, Turkey
İ Akin
Affiliation:
First ENT Clinic, Ministry of Health Dışkapı Yıldırım Beyazıt Training and Research Hospital, Ankara, Turkey
*
Address for correspondence: Dr Sibel Alicura Tokgöz, First ENT Clinic, Dışkapı Yldırım Beyazıt Training and Research Hospital, Ministry of Health, Ankara, Turkey E-mail: salicura@yahoo.com
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Abstract

Objective:

This experimental study aimed to investigate the effects of vitamins E, B and C and l-carnitine in preventing cisplatin-induced ototoxicity.

Methods:

Twenty-five adult, male, Wistar albino rats were randomly allocated to receive intraperitoneal cisplatin either alone or preceded by vitamins B, E or C or l-carnitine. Auditory brainstem response (i.e. hearing thresholds and wave I–IV intervals) and distortion product otoacoustic emissions (i.e. signal-to-noise ratios) were recorded before and 72 hours after cisplatin administration.

Results:

The following statistically significant differences were seen: control group pre- vs post-treatment wave I–IV interval values (p < 0.05); control vs vitamin E and B groups' I–IV interval values (p < 0.05); control vs other groups' hearing thresholds; vitamin E vs vitamin B and C and l-carnitine groups' hearing thresholds (p < 0.05); and vitamin B vs vitamin C and l-carnitine groups' hearing thresholds (p < 0.05). Statistically significant decreases were seen when comparing the initial and final signal-to-noise ratios in the control, vitamin B and l-carnitine groups (2000 and 3000 Hz; p < 0.01), and the initial and final signal-to-noise ratios in the control group (at 4000 Hz; p < 0.01).

Conclusion:

Vitamins B, E and C and l-carnitine appear to reduce cisplatin-induced ototoxicity in rats. The use of such additional treatments to decrease cisplatin-induced ototoxicity in humans is still under discussion.

Keywords

OtotoxicityCisplatinEar, InnerAntioxidantsRat
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 126 , Issue 5 , May 2012 , pp. 464 - 469
Copyright
Copyright © JLO (1984) Limited 2012

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References

1Jordan, J, Schwade, ND, Truelson, JM. Fosfomycin does not inhibit the tumoricidal efficacy of cisplatinum. Laryngoscope 1999;109:1259–62CrossRefGoogle Scholar
2Fram, RJ. Cisplatin and platinum analogues: recent advances. Curr Opin Oncol 1992;4:1073–9CrossRefGoogle ScholarPubMed
3Guneri, EA, Serbetçioglu, B, Ikiz, AO, Guneri, A, Ceryan, K. TEOAE monitoring of cisplatin induced ototoxicity in guinea pigs: the protective effect of vitamin B treatment. Auris Nasus Larynx 2001;28:9–14CrossRefGoogle ScholarPubMed
4Feghali, JG, Liu, W, Water, TRVD. L-N-acetyl-cysteine protection against cisplatin induced auditory neuronal and hair cell toxicity. Laryngoscope 2001;111:1147–55CrossRefGoogle ScholarPubMed
5Cardinaal, RM, De Groot, JCMJ, Huizing, EH, Veldman, JE, Smoorenburg, GF. Dose-dependent effect of 8-day cisplatin administration upon the morphology of the albino guinea pig cochlea. Hear Res 2000;144:135–46CrossRefGoogle ScholarPubMed
6Blakley, BW, Cohen, JI, Doolittle, ND, Muldoon, LL, Campbell, KC, Dickey, DT et al. Strategies for prevention of toxicity caused by platinum-based chemotherapy: review and summary of the annual meeting of the Blood–Brain Barrier Disruption Program, Gleneden Beach, Oregon, March 10, 2001. Laryngoscope 2002;112:1997–2001CrossRefGoogle Scholar
7Lynch, ED, Gu, R, Pierce, C, Kil, J. Reduction of acute cisplatin ototoxicity and nephrotoxicity in rats by oral administration of allopurinol and ebselen. Hear Res 2005;201:81–9CrossRefGoogle ScholarPubMed
8Rybak, LP, Whitworth, C, Somani, S. Application of antioxidants and other agents to prevent cisplatin ototoxicity. Laryngoscope 1999;109:1740–4CrossRefGoogle ScholarPubMed
9Ekborn, A, Anderssen, A, Ehrsson, H, Eksborg, S, Laurell, G, Wallin, I. Cisplatin-induced hearing loss: influence of the mode of drug administration in the guinea pig. Hear Res 2000;140:38–44CrossRefGoogle ScholarPubMed
10Schweitzer, VG. Cisplatin-induced ototoxicity: effect of pigmentation and inhibitory agents. Laryngoscope 1993;103:1–52Google ScholarPubMed
11Sakamoto, M, Kaga, K, Kamio, T. Extended high-frequency ototoxicity induced by the first administration of cisplatin. Otolaryngol Head Neck Surg 2000;122:828–33Google ScholarPubMed
12Reddel, RR, Kefford, RF, Grant, JM, Coats, AS, Fox, RM, Tattersall, MHN. Ototoxicity in patients receiving cisplatin: importance of dose and method of drug administration. Cancer Treat Rep 1982;66:19–23Google ScholarPubMed
13Rybak, LP, Husain, K, Evenson, L, Morris, C, Whitworth, C, Somani, SM. Protection by 4-methylthiobenzoic acid against cisplatin-induced ototoxicity: antioxidant system. Pharmacol Toxicol 1997;81:173–9CrossRefGoogle ScholarPubMed
14Saito, T, Aran, JM. Comparative ototoxicity of cisplatin during acute and chronic treatment. ORL J Otorhinolaryngol Relat Spec 1994;56:315–20CrossRefGoogle ScholarPubMed
15Giordano, P, Lorito, G, Ciorba, A, Martini, A, Hatzopoulos, S. Protection against cisplatin ototoxicity in a Sprague-Dawley rat animal model. Acta Otolaryngol Ital 2006;26:198–207Google Scholar
16Blumenreich, MS, Woodcock, TM, Richman, SP, Jones, MK, Gentile, PS, Kubota, TT et al. High dose cisplatin in patients with advanced malignancies. Cancer 1985;55:1118–223.0.CO;2-5>CrossRefGoogle ScholarPubMed
17Li, G, Frenz, DA, Brahmblatt, S, Feghali, JG, Ruben, RJ, Berggren, D et al. Round window membrane delivery of L-methionine provides protection from cisplatin ototoxicity without compromising chemotherapeutic efficacy. Neurotoxicology 2001;22:163–76CrossRefGoogle ScholarPubMed
18Ajith, TA, Usha, S, Nivitha, V. Ascorbic acid and alpha-tocopherol protect anticancer drug cisplatin induced nephrotoxicity in mice: a comparative study. Clin Chim Acta 2007;375:82–6CrossRefGoogle ScholarPubMed
19Ajith, TA, Abhishek, G, Roshny, D, Sudheesh, NP. Co-supplementation of single and multi doses of vitamins C and E ameliorates cisplatin-induced acute renal failure in mice. Exp Toxicol Pathol 2009;61:565–71CrossRefGoogle Scholar
20Wimmer, C, Mees, K, Stumpf, P, Welsch, U, Reichel, O, Suckfüll, M. Round window application of D-methionine, sodium thiosulfate, brain-derived neurotrophic factor, and fibroblast growth factor-2 in cisplatin-induced ototoxicity. Otol Neurotol 2004;25:33–40CrossRefGoogle ScholarPubMed
21Campbell, KCM, Larsen, DL, Meech, RP, Rybak, LP, Hughes, LF. Glutathione ester but not glutathione protects against cisplatin-induced ototoxicity in a rat model. J Am Acad Audiol 2003;14:124–33Google Scholar
22Hyppolito, MA, de Oliveira, JA, Rossato, M. Cisplatin ototoxicity and otoprotection with sodium salicylate. Eur Arch Otorhinolaryngol 2006;263:798–803CrossRefGoogle ScholarPubMed
23Kamimura, T, Whitworth, CA, Rybak, LP. Effect of 4-methylthiobenzoic acid on cisplatin-induced ototoxicity in the rat. Hear Res 1999;131:117–27CrossRefGoogle ScholarPubMed
24Dehne, N, Lautermann, J, Petrat, F, Rauen, U, de Groot, H. Cisplatin ototoxicity: involvement of iron and enhanced formation of superoxide anion radicals. Toxicol Appl Pharmacol 2001;174:27–34CrossRefGoogle ScholarPubMed
25Rybak, LP, Husain, K, Morris, C, Whitworth, C, Somani, S. Effect of protective agents against cisplatin ototoxicity. Am J Otol 2000;21:513–20Google ScholarPubMed
26Weijl, NI, Hopman, GD, Wipkink-Bakker, A, Lentjes, EG, Berger, HM, Cleton, FJ et al. Cisplatin combination chemotherapy induces a fall in plasma anti-oxidants of cancer patients. Ann Oncol 1998;9:1331–7CrossRefGoogle ScholarPubMed
27Teranishi, M, Nakashima, T, Wakabayashi, T. Effects of alpha-tocopherol on cisplatin-induced ototoxicity in guinea pigs. Hear Res 2001;151:61–70CrossRefGoogle ScholarPubMed
28Hatano, M, Uramoto, N, Okabe, Y, Furukawa, M, Ito, M. Vitamin E and vitamin C in the treatment of idiopathic sudden sensorineural hearing loss. Acta Otolaryngol 2008;128:116–21CrossRefGoogle ScholarPubMed
29Freitas, MR, Silva, VC, Brito, GA, Carvalho, JV Jr, Gomes, RM Jr , Ribeiro R de, A. Distortion-product otoacoustic emissions and auditory brainstem responses sensitivity assessment in cisplatin-induced ototoxicity in rats. Braz J Otorhinolaryngol 2009;75:476–84Google ScholarPubMed
30Shaw, NA. The auditory evoked potential in the rat – a review. Prog Neurobiol 1988;31:19–45CrossRefGoogle ScholarPubMed
31Kalkanis, JG, Whitworth, C, Rybak, LP. Vitamin E reduces cisplatin ototoxicity. Laryngoscope 2004;114:538–42CrossRefGoogle ScholarPubMed
32Leonetti, C, Biroccio, A, Gabellini, C, Scarsella, M, Maresca, V, Flori, E et al. Alpha-tocopherol protects against cisplatin-induced toxicity without interfering with antitumor efficacy. Int J Cancer 2003;104:243–50CrossRefGoogle ScholarPubMed
33Seidman, MD, Khan, MJ, Bai, U, Shirwany, N, Quirk, WS. Biologic activity of mitochondrial metabolites on aging and age-related hearing loss. Am J Otol 2000;21:161–7CrossRefGoogle ScholarPubMed
34Bielefeld, EC, Coling, D, Chen, GD, Henderson, D. Multiple dosing strategies with acetyl L-carnitine (ALCAR) fail to alter age-related hearing loss in the Fischer 344/NHsd rat. J Negat Results Biomed 2008;7:4CrossRefGoogle ScholarPubMed
35Coleman, JK, Kopke, RD, Liu, J, Ge, X, Harper, EA, Jones, GE et al. Pharmacological rescue of noise induced hearing loss using N-acetylcysteine and acetyl-L-carnitine. Hear Res 2007;226:104–13CrossRefGoogle ScholarPubMed