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Comparison of bacterial communities associated with Xestospongia testudinaria, sediment and seawater in a Singaporean coral reef ecosystem

Published online by Cambridge University Press:  02 April 2018

A.C.C. Pires ,
D.F.R. Cleary ,
A.R.M. Polónia ,
S.C. Lim ,
N.J. De Voogd ,
V. Oliveira  and
N.C.M. Gomes
A.C.C. Pires
Affiliation:
Department of Biology and CESAM, University of Aveiro, Aveiro, Portugal
D.F.R. Cleary
Affiliation:
Department of Biology and CESAM, University of Aveiro, Aveiro, Portugal
A.R.M. Polónia
Affiliation:
Department of Biology and CESAM, University of Aveiro, Aveiro, Portugal
S.C. Lim
Affiliation:
Tropical Marine Science Institute, National University of Singapore, Singapore
N.J. De Voogd
Affiliation:
Naturalis Biodiversity Center, Leiden, the Netherlands
V. Oliveira
Affiliation:
Department of Biology and CESAM, University of Aveiro, Aveiro, Portugal
N.C.M. Gomes*
Affiliation:
Department of Biology and CESAM, University of Aveiro, Aveiro, Portugal
*
Correspondence should be addressed to: N. C. M. Gomes, CESAM – Centre for Environmental and Marine Studies, University of Aveiro, 3810-193 Aveiro, Portugal email: gomesncm@ua.pt
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Abstract

Despite alterations caused by anthropogenic activities in Singaporean coral reefs, the sponge communities are quite diverse and Xestospongia testudinaria is one of the most common sponge species. In the present study, we used 16S rRNA gene barcoded pyrosequencing to characterize and compare bacterial communities from different biotopes (sponge, seawater and sediment) and to identify dominant bacterial symbionts of X. testudinaria in a Singaporean coral reef ecosystem. Our results showed that biotope appears to affect the richness, composition and abundance of bacterial communities. Proteobacteria was the most abundant phylum in sediment and seawater whilst Chloroflexi was more abundant in X. testudinaria. Members of the order Caldilineales (fermentation of organic substrates), Chromatiales (purple sulphur bacteria), Rhodospirillales (purple non-sulphur bacteria) and Syntrophobacterales (sulphate-reducing bacteria) were relatively more abundant in X. testudinaria samples.

Keywords

Community compositioncoral reefspyrosequencingSingaporeXestospongia testudinaria
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 99 , Issue 2 , March 2019 , pp. 331 - 342
Copyright
Copyright © Marine Biological Association of the United Kingdom 2018 

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