Skip to main content
×
Home

Characterization of a humic acid extracted from marine sediment and its influence on the growth of marine diatoms

  • Marcela V. Martin (a1), Christina Gebühr (a2), Daniel O. Mártire (a1) and Karen H. Wiltshire (a3)
Abstract

Due to the input of humic substances from freshwater run-off into the marine habitat, the influences of such substances on marine organisms should not be neglected. We here investigate the effect of a humic acid (HA) extract from the North Sea, characterized by spectroscopic techniques and carboxylic and phenolic group content, on the growth of different algae. Two benthic pennate diatoms isolated from the Baltic Sea, Navicula ramosissima (C. Agardh) Cleve, 1895 and Entomoneis paludosa (W. Smith) Reimer, 1975, as well as two tychopelagic centric diatoms isolated from the North Sea, Melosira nummuloides C. Agardh, 1824 and Paralia sulcata (Ehrenberg) Cleve, 1873, were employed. The concentrations of pigments (fucoxanthin, diadinoxanthin, chlorophyll-a and β-carotene) and nutrients were also measured. Adding low concentrations of naturally humic substances (5.5 mg of C l−1) to the algae cultures resulted in enhanced growth rates compared to the control experiments, possibly due to the increase in the bioavailability of trace metals or other nutrients.

Copyright
Corresponding author
Correspondence should be addressed to: M.V. Martin, Casilla de correo 16, sucursal 4 (1900), La Plata, Argentina email: mmartin@inifta.unlp.edu.ar.
References
Hide All
Aiken G.R., McKnight D.M., Wershaw R.L. and MacCarthy P. (1985) An introduction to humic substances in soils, sediments and waters. In Aiken G.R., McKnight D.M., Wershaw R.L. and MacCarthy P. (eds) Humic substances in soil, sediment and water. New York: John Wiley & Sons, pp. 19.
Amir S., Hafidi M., Bailly J-R. and Revel J-C. (2003) Characterization of humic acids extracted from sewage sludge during composting and of their Sephadex® gel fractions. Agronomie 23, 269275.
Andjelkovic T., Perovic J., Purenovic M., Бlagojevic S., Nikolic R., Andjelkovic D. and Bojic A. (2006) Spectroscopic and potentiometric studies on derivatized natural humic acid. Analytical Sciences 22, 15531558.
Bährs H. and Steinberg C.E.W. (2012) Impact of two different humic substances on selected coccal green algae and Cyanobacteria-changes in growth and photosynthetic performance. Environmental Science and Pollution Research 19, 335346.
Beck M., Olaf Dellwig O., Fischer S., Schnetger B. and Brumsack H.-J. (2012) Trace metal geochemistry of organic carbon-rich watercourses draining the NW German coast. Estuarine, Coastal and Shelf Science 104/105, 6679.
Beutler M., Wiltshire K.H., Meyer B., Moldaenke C., Luring C., Meyerhofer M., Hansen U. P. and Dau H. (2002) A fluorometric method for the differentiation of algal populations in vivo and in situ. Photosynthesis Research 72, 3953.
Bosio G.N., David Gara P., García Einschlag F.S., Gonzalez M.C., del Panno M.T. and Mártire D.O. (2008) Photodegradation of soil organic matter and its effect on gram (-) bacterial growth. Photochemistry & Photobiology 84, 11261132.
Campbell P.G.C. (1995) Interactions between trace metals and aquatic organisms: A critique of the free-ion activity model. In Tessier A. and Turner D.R. (eds) Metal speciation and bioavailability in aquatic systems. New York: John Wiley & Sons, pp. 45102.
Carder K.L., Steward R.G., Harvey G.R. and Ortner P.B. (1989) Marine humic and fulvic acids: their effects on remote sensing of ocean chlrophyll. Limnology and Oceanography 34, 6881.
Carlos L., Pedersen B.W., Ogilby P.R. and Mártire D.O. (2011) The role of humic acid aggregation on the kinetics of photosensitized singlet oxygen production and decay. Photochemical and Photobiological Science 10, 10801086.
Chen J., Gu B., Leboeuf B.J., Pan H. and Dai S. (2002) Spectroscopic characterization of the structural and functional properties of natural organic matter fractions. Chemosphere 48, 5968.
Chen M. and Wang W-X. (2008) Accelerated uptake by phytoplankton of iron bound to humic acids. Aquatic Biology 3, 155166.
Chen W., Westerhoff P., Leenheer J.A. and Booksh K. (2003) Fluorescence excitation–emission matrix regional integration to quantify spectra for dissolved organic matter. Environmental Science and Technology 37, 57015710.
Chen Y., Senesi N. and Schnitzer M. (1977) Information provided on humic substances by E4–E6 ratios. Soil Science Society of America Journal 41, 352358.
Chen Z., Porcher C., Campbell P.G.C. and Fortin C. (2013) Influence of humic acid on algal uptake and toxicity of ionic silver. Environmental Science and Technology 47, 88358842.
Chin Y.P., Aiken G. and O'Loughlin E. (1994) Molecular weight, polydispersity and spectroscopic properties of aquatic humic substances. Environmental Science and Technology 28, 18531858.
Coble P.G. (1996) Characterization of marine and terrestrial DOM in seawater using excitation–emission matrix spectroscopy. Marine Chemistry 51, 325346.
Daehnick A.E., Sullivan M.J. and Moncreiff C.A. (1992) Primary production of the sand microflora in seagrass beds of Mississippi Sound. Botanica Marina 35, 131139.
Del Vecchio R. and Blough N.V. (2002) Photobleaching of chromophoric dissolved organic matter in natural waters: kinetics and modeling. Marine Chemistry 78, 231253.
Franke H-D., Bucholz F. and Wiltshire K.H. (2004) Ecological long-term research at Helgoland (German Bight, North Sea): retrospect and prospect—an introduction. Helgoland Marine Research 58, 223229.
Frost B.W. (1972) Effects of size and concentration of food particles on the feeding behavior of the marine planktonic copepod Calanus pacificus. Limnology and Oceanography 14, 805815.
Ghabbour E.A. and Davies G. (2005) Humic substances: molecular details and applications in land and water conservation. New York: Taylor & Francis.
Graham L.E. and Wilcox L.W. (2000). Ochrophytes. Part 2. Diatoms. In Algae. Upper Saddle River, NJ: Prentice-Hall, Chapter 12.
Grasshoff K. (1976) Methods of seawater analysis. Weinheim: Verlag Chemie.
Guillard R.R.L. and Ryther J.H. (1962) Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonula confervacea Cleve. Canadian Journal of Microbiology 8, 229239.
Hellebost J.A. and Guillaro R.R.L. (1967) Uptake specificity for organic substrates by the marine diatom Melosira nummuloides. Journal of Phycology 3, 132136.
Hillebrand H., Dürselen C.D., Kirschtel D., Pollingher U. and Zohary T. (1999) Biovolume calculating for pelagic and benthic microalgae. Journal of Phycology 35, 403424.
Imai A., Fukushima T. and Matsushige K. (1999) Effects of iron limitation and aquatic humic substances on the growth of Microcystis aeruginosa. Canadian Jourmal of Fisheries and Aquatic Science 56, 19291937.
IHSS (Internacional Humic Substances Society) (2008) Available at: http://www.humicsubstances.org/
Knefelkamp B., Carstens K. and Wiltshire K.H. (2007) Comparison of different filter types on chlorophyll-a retention and nutrient measurements. Journal of Experimental Marine Biology and Ecology 345, 6170.
Lguirati A., Ait Baddi G., El Mousadik A., Gilard V., Revel J.C. and Hafidi M. (2005) Analysis of humic acids from aerated and non-aerated urban landfill composts. International Biodeterioration & Biodegradation 56, 816.
Lund W. (1990) The complexation of metal ions by humic substances in natural waters. In Broekaert JAC, Güçer S. and Adams F. (eds) Metal speciation in the environment. Berlin: Springer-Verlag, pp. 4356.
MacIntyre H.L., Geider R.J. and Miller D.C. (1996) Microphytobenthos: the ecological role of the ‘secret garden’ of unvegetated, shallow-water marine habitats. 1. Distribution, abundance and primary production. Estuaries 19, 186201.
McLean R.O., Corrigan J. and Webster J. (1981) Heterotrophic nutrition in Melosira nummuloides, a possible role in affecting distribution in the Clyde Estuary. European Journal of Phycology 16, 95106.
McQuoid M.R. and Hobson L.A. (1998) Assessment of palaeoenvironmental conditions on Southern Vancouver Island, British Columbia, Canada, using the marine tychopankter Paralia sulcata. Diatom Research 13, 311321.
McQuoid M.R. and Nordberg K. (2003) The diatom Paralia sulcata as an environmental indicator species in coastal sediments. Estuarine, Coastal and Shelf Science 56, 339354.
Mignone R.A., Martin M.V., Morán Vieyra F.E., Palazzi V.I., López de Mishima B., Mártire D.O. and Borsarelli C.D. (2012) Modulation of optical properties of dissolved humic substances by their origin and molecular complexity. Photochemistry and Photobiology 88, 792800. [Special Issue on the 21st Conference of the IAPS.]
Moreda-Piñeiro A., Bermejo-Barrera A. and Bermejo-Barrera P. (2004) New trends involving the use of ultrasound energy for the extraction of humic substances from marine sediments. Analytica Chimica Acta 524, 97107.
Moreda-Piñeiro A., Seco-Gesto E.M., Bermejo-Barrera A. and Bermejo-Barrera P. (2006) Characterization of surface marine sediments from Ría de Arousa estuary according to extractable humic matter content. Chemosphere 64, 866873.
Nardi S., Pizzeghello D., Muscolo A. and Vianello A. (2002) Physiological effects of humic substances on higher plants. Soil Biology & Biochemistry 34, 15271536.
Parlanti E., Morin B. and Vache L. (2002) Combined 3D-spectrofluorometry, high performance liquid chromatography and capillary electrophoresis for the characterization of dissolved organic matter in natural waters. Organic Geochemistry 33, 221223.
Pinckney J.L. and Zingmark R.G. (1993) Modelling the annual production of intertidal benthic microalgae in estuarine ecosystems. Journal of Phycology 29, 396407.
Prakash A. and Rashid M.A. (1968) Influence of humic substances on the growth of marine phytoplankton: dinoflagellates. Limnology and Oceanography 13, 598606.
Prakash A., Rashid M.A., Jensen A. and Subbarao D.V. (1973) Influence of humic substances on growth of marine phytoplankton–diatoms. Limnology and Oceanography 18, 516524.
Prokhotskaya V.Y. and Steinberg C.E.W. (2007) Differential sensitivity of a coccal green algae and a cyanobacterial species to dissolved natural organic matter (NOM). Environmental Science and Pollution Research 14, 1118.
Rashid M.A. (1971) Role of humic acids of marine origin and their different molecular weight fractions in complexing di- and trivalent metals. Soil Science 111, 298306.
Roelofs A.K. (1984) Distributional patterns and variation of valve diameter of Paralia sulcata in surface sediments of Southern British Columbia Inlets. Estuarine, Coastal and Shelf Science 18, 165176.
Sánchez-Marín P. and Beiras R. (2011) Adsorption of different types of dissolved organic matter to marine phytoplankton and implications for phytoplankton growth and Pb bioavailability. Journal of Plankton Research 33, 13961409.
Steinberg C.E.W., Kamara S., Prokhotskaya V.Y., Manusadzianas L., Karasyova T.A., Timofeyev M.A., Jie Z., Paul A., Meinelt T., Farjalla V.F., Matsuo A.Y.O., Burnison B.K. and Menzel R. (2006) Dissolved humic substances: Ecological driving forces from the individual to the ecosystem level? Freshwater Biology 51, 11891210.
Schnitzer M. and Khan S.U. (1972) Humic subtances in the enviroment. New York: Marcel Dekker.
Schnitzer M. and Gupta U.C. (1965) Determination of acidity in soil organic matter. Soil Science Society of America Proceedings 29, 274277.
Sierra M.M.D., Donard O.F.X. and Lamotte M. (1997) Behaviour of dissolved fluorescent organic matter during estuarine mixing. Marine Chemistry 58, 5158.
Sierra M.M.D., Donard O.F.X., Lamotte M., Belin C. and Ewald M. (1994) Fluorescence spectroscopy of coastal and marine waters. Marine Chemistry 47, 127144.
Sierra M.M.D., Giovanela M., Parlanti E. and Soriano-Sierra E.J. (2005) Fluorescence fingerprint of fulvic and humic acids from varied origins as viewed by single-scan and excitation/emission matrix techniques. Chemopshere 58, 715733.
Silva M.R. (1996) Estudos potenciométricos e fluorimétricos dos equilíbrios ácido-básicos e da complexação de metáis com o Obisdien e as substãncias húmicas. Contaminação pelos metais em sedimentos da Ilha de Santa Catarina (Brasil). PhD thesis. Universidad e Federal de Santa Catarina, Brazil.
Song Z., Chouparova E., Jones K.W., Feng H. and Marinkovic N.S. (2001) FTIR investigation of sediments from NY/NJ Harbor, San Diego Bay and the Venetian Lagoon. NSLS Activity Report, Z-112-Z-116.
Steinberg C.E.W., Kamara S., Prokhotskaya V.Y., Manusadzianas L., Karasyova T.A., Timofeyev M.A., Jie Z., Paul A., Meinelt T., Farjalla V.F., Matsuo A.Y.O, Burnison B.K. and Menzel R. (2006) Dissolved humic substances. Ecological driving forces from the individual to the ecosystem level? Freshwater Biology 51, 11891210.
Wilkinson M., Henderson A.R. and Wilkinson C. (1976) Distribution of attached algae in estuaries. Marine Pollution Bulletin 7, 183.
Wiltshire K.H. (2000) Algae and associated pigments of intertidal sediments; new observations and methods. Limnologica 30, 205214.
Wiltshire K.H., Harsdorf S., Smidt B., Blocker G., Reuter R. and Schroeder F. (1998) The determination of algal biomass (as chlorophyll) in suspended matter from the Elbe estuary and the German Bight: a comparison of high-performance liquid chromatography, delayed fluorescence and prompt fluorescence methods. Journal of Experimental Marine Biology and Ecology 222, 113131.
Wiltshire K.H. and Manly B.F.J. (2004) The warming trend at Helgoland Roads, North Sea: phytoplankton response. Helgoland Marine Research 58, 269273.
Wolff W.J. (1979) Flora and vegetation of the Wadden Sea. Report 3. In Final Report of the Section Marine Biology of the Wadden Sea. Rotterdam: A.A. Balkema.
Zhang Y., Du J., Zhang F., Yu Y. and Zhang J. (2011) Chemical characterization of humic substances isolated from mangrove swamp sediments: the Qinglan area of Hainan Island, China. Estuarine, Coastal and Shelf Science 92, 180187.
Zong Y. (1997) Implications of Paralia sulcata abundance in Scottish isolation basins. Diatom Research 12, 125150.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Journal of the Marine Biological Association of the United Kingdom
  • ISSN: 0025-3154
  • EISSN: 1469-7769
  • URL: /core/journals/journal-of-the-marine-biological-association-of-the-united-kingdom
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords:

Metrics

Full text views

Total number of HTML views: 6
Total number of PDF views: 18 *
Loading metrics...

Abstract views

Total abstract views: 132 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 23rd November 2017. This data will be updated every 24 hours.