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Characterization of a humic acid extracted from marine sediment and its influence on the growth of marine diatoms

  • Marcela V. Martin (a1), Christina Gebühr (a2), Daniel O. Mártire (a1) and Karen H. Wiltshire (a3)

Due to the input of humic substances from freshwater run-off into the marine habitat, the influences of such substances on marine organisms should not be neglected. We here investigate the effect of a humic acid (HA) extract from the North Sea, characterized by spectroscopic techniques and carboxylic and phenolic group content, on the growth of different algae. Two benthic pennate diatoms isolated from the Baltic Sea, Navicula ramosissima (C. Agardh) Cleve, 1895 and Entomoneis paludosa (W. Smith) Reimer, 1975, as well as two tychopelagic centric diatoms isolated from the North Sea, Melosira nummuloides C. Agardh, 1824 and Paralia sulcata (Ehrenberg) Cleve, 1873, were employed. The concentrations of pigments (fucoxanthin, diadinoxanthin, chlorophyll-a and β-carotene) and nutrients were also measured. Adding low concentrations of naturally humic substances (5.5 mg of C l−1) to the algae cultures resulted in enhanced growth rates compared to the control experiments, possibly due to the increase in the bioavailability of trace metals or other nutrients.

Corresponding author
Correspondence should be addressed to: M.V. Martin, Casilla de correo 16, sucursal 4 (1900), La Plata, Argentina email:
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S. Amir , M. Hafidi , J-R. Bailly and J-C. Revel (2003) Characterization of humic acids extracted from sewage sludge during composting and of their Sephadex® gel fractions. Agronomie 23, 269275.

T. Andjelkovic , J. Perovic , M. Purenovic , S. Бlagojevic , R. Nikolic , D. Andjelkovic and A. Bojic (2006) Spectroscopic and potentiometric studies on derivatized natural humic acid. Analytical Sciences 22, 15531558.

H. Bährs and C.E.W. Steinberg (2012) Impact of two different humic substances on selected coccal green algae and Cyanobacteria-changes in growth and photosynthetic performance. Environmental Science and Pollution Research 19, 335346.

M. Beck , Dellwig O. Olaf , S. Fischer , B. Schnetger and H.-J. Brumsack (2012) Trace metal geochemistry of organic carbon-rich watercourses draining the NW German coast. Estuarine, Coastal and Shelf Science 104/105, 6679.

G.N. Bosio , P. David Gara , F.S. García Einschlag , M.C. Gonzalez , M.T. del Panno and D.O. Mártire (2008) Photodegradation of soil organic matter and its effect on gram (-) bacterial growth. Photochemistry & Photobiology 84, 11261132.

K.L. Carder , R.G. Steward , G.R. Harvey and P.B. Ortner (1989) Marine humic and fulvic acids: their effects on remote sensing of ocean chlrophyll. Limnology and Oceanography 34, 6881.

L. Carlos , B.W. Pedersen , P.R. Ogilby and D.O. Mártire (2011) The role of humic acid aggregation on the kinetics of photosensitized singlet oxygen production and decay. Photochemical and Photobiological Science 10, 10801086.

J. Chen , B. Gu , B.J. Leboeuf , H. Pan and S. Dai (2002) Spectroscopic characterization of the structural and functional properties of natural organic matter fractions. Chemosphere 48, 5968.

M. Chen and W-X. Wang (2008) Accelerated uptake by phytoplankton of iron bound to humic acids. Aquatic Biology 3, 155166.

Y.P. Chin , G. Aiken and E. O'Loughlin (1994) Molecular weight, polydispersity and spectroscopic properties of aquatic humic substances. Environmental Science and Technology 28, 18531858.

P.G. Coble (1996) Characterization of marine and terrestrial DOM in seawater using excitation–emission matrix spectroscopy. Marine Chemistry 51, 325346.

A.E. Daehnick , M.J. Sullivan and C.A. Moncreiff (1992) Primary production of the sand microflora in seagrass beds of Mississippi Sound. Botanica Marina 35, 131139.

R. Del Vecchio and N.V. Blough (2002) Photobleaching of chromophoric dissolved organic matter in natural waters: kinetics and modeling. Marine Chemistry 78, 231253.

H-D. Franke , F. Bucholz and K.H. Wiltshire (2004) Ecological long-term research at Helgoland (German Bight, North Sea): retrospect and prospect—an introduction. Helgoland Marine Research 58, 223229.

R.R.L. Guillard and J.H. Ryther (1962) Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonula confervacea Cleve. Canadian Journal of Microbiology 8, 229239.

J.A. Hellebost and R.R.L. Guillaro (1967) Uptake specificity for organic substrates by the marine diatom Melosira nummuloides. Journal of Phycology 3, 132136.

A. Imai , T. Fukushima and K. Matsushige (1999) Effects of iron limitation and aquatic humic substances on the growth of Microcystis aeruginosa. Canadian Jourmal of Fisheries and Aquatic Science 56, 19291937.

B. Knefelkamp , K. Carstens and K.H. Wiltshire (2007) Comparison of different filter types on chlorophyll-a retention and nutrient measurements. Journal of Experimental Marine Biology and Ecology 345, 6170.

A. Lguirati , G. Ait Baddi , A. El Mousadik , V. Gilard , J.C. Revel and M. Hafidi (2005) Analysis of humic acids from aerated and non-aerated urban landfill composts. International Biodeterioration & Biodegradation 56, 816.

W. Lund (1990) The complexation of metal ions by humic substances in natural waters. In JAC Broekaert , S. Güçer and F. Adams (eds) Metal speciation in the environment. Berlin: Springer-Verlag, pp. 4356.

H.L. MacIntyre , R.J. Geider and D.C. Miller (1996) Microphytobenthos: the ecological role of the ‘secret garden’ of unvegetated, shallow-water marine habitats. 1. Distribution, abundance and primary production. Estuaries 19, 186201.

M.R. McQuoid and L.A. Hobson (1998) Assessment of palaeoenvironmental conditions on Southern Vancouver Island, British Columbia, Canada, using the marine tychopankter Paralia sulcata. Diatom Research 13, 311321.

M.R. McQuoid and K. Nordberg (2003) The diatom Paralia sulcata as an environmental indicator species in coastal sediments. Estuarine, Coastal and Shelf Science 56, 339354.

A. Moreda-Piñeiro , A. Bermejo-Barrera and P. Bermejo-Barrera (2004) New trends involving the use of ultrasound energy for the extraction of humic substances from marine sediments. Analytica Chimica Acta 524, 97107.

R.A. Mignone , M.V. Martin , F.E. Morán Vieyra , V.I. Palazzi , B. López de Mishima , D.O. Mártire and C.D. Borsarelli (2012) Modulation of optical properties of dissolved humic substances by their origin and molecular complexity. Photochemistry and Photobiology 88, 792800. [Special Issue on the 21st Conference of the IAPS.]

A. Moreda-Piñeiro , E.M. Seco-Gesto , A. Bermejo-Barrera and P. Bermejo-Barrera (2006) Characterization of surface marine sediments from Ría de Arousa estuary according to extractable humic matter content. Chemosphere 64, 866873.

S. Nardi , D. Pizzeghello , A. Muscolo and A. Vianello (2002) Physiological effects of humic substances on higher plants. Soil Biology & Biochemistry 34, 15271536.

E. Parlanti , B. Morin and L. Vache (2002) Combined 3D-spectrofluorometry, high performance liquid chromatography and capillary electrophoresis for the characterization of dissolved organic matter in natural waters. Organic Geochemistry 33, 221223.

J.L. Pinckney and R.G. Zingmark (1993) Modelling the annual production of intertidal benthic microalgae in estuarine ecosystems. Journal of Phycology 29, 396407.

A. Prakash and M.A. Rashid (1968) Influence of humic substances on the growth of marine phytoplankton: dinoflagellates. Limnology and Oceanography 13, 598606.

A. Prakash , M.A. Rashid , A. Jensen and D.V. Subbarao (1973) Influence of humic substances on growth of marine phytoplankton–diatoms. Limnology and Oceanography 18, 516524.

V.Y. Prokhotskaya and C.E.W. Steinberg (2007) Differential sensitivity of a coccal green algae and a cyanobacterial species to dissolved natural organic matter (NOM). Environmental Science and Pollution Research 14, 1118.

M.A. Rashid (1971) Role of humic acids of marine origin and their different molecular weight fractions in complexing di- and trivalent metals. Soil Science 111, 298306.

A.K. Roelofs (1984) Distributional patterns and variation of valve diameter of Paralia sulcata in surface sediments of Southern British Columbia Inlets. Estuarine, Coastal and Shelf Science 18, 165176.

P. Sánchez-Marín and R. Beiras (2011) Adsorption of different types of dissolved organic matter to marine phytoplankton and implications for phytoplankton growth and Pb bioavailability. Journal of Plankton Research 33, 13961409.

M.M.D. Sierra , O.F.X. Donard and M. Lamotte (1997) Behaviour of dissolved fluorescent organic matter during estuarine mixing. Marine Chemistry 58, 5158.

M.M.D. Sierra , O.F.X. Donard , M. Lamotte , C. Belin and M. Ewald (1994) Fluorescence spectroscopy of coastal and marine waters. Marine Chemistry 47, 127144.

M.M.D. Sierra , M. Giovanela , E. Parlanti and E.J. Soriano-Sierra (2005) Fluorescence fingerprint of fulvic and humic acids from varied origins as viewed by single-scan and excitation/emission matrix techniques. Chemopshere 58, 715733.

C.E.W. Steinberg , S. Kamara , V.Y. Prokhotskaya , L. Manusadzianas , T.A. Karasyova , M.A. Timofeyev , Z. Jie , A. Paul , T. Meinelt , V.F. Farjalla , A.Y.O Matsuo , B.K. Burnison and R. Menzel (2006) Dissolved humic substances. Ecological driving forces from the individual to the ecosystem level? Freshwater Biology 51, 11891210.

M. Wilkinson , A.R. Henderson and C. Wilkinson (1976) Distribution of attached algae in estuaries. Marine Pollution Bulletin 7, 183.

K.H. Wiltshire (2000) Algae and associated pigments of intertidal sediments; new observations and methods. Limnologica 30, 205214.

K.H. Wiltshire , S. Harsdorf , B. Smidt , G. Blocker , R. Reuter and F. Schroeder (1998) The determination of algal biomass (as chlorophyll) in suspended matter from the Elbe estuary and the German Bight: a comparison of high-performance liquid chromatography, delayed fluorescence and prompt fluorescence methods. Journal of Experimental Marine Biology and Ecology 222, 113131.

K.H. Wiltshire and B.F.J. Manly (2004) The warming trend at Helgoland Roads, North Sea: phytoplankton response. Helgoland Marine Research 58, 269273.

Y. Zhang , J. Du , F. Zhang , Y. Yu and J. Zhang (2011) Chemical characterization of humic substances isolated from mangrove swamp sediments: the Qinglan area of Hainan Island, China. Estuarine, Coastal and Shelf Science 92, 180187.

Y. Zong (1997) Implications of Paralia sulcata abundance in Scottish isolation basins. Diatom Research 12, 125150.

M. Beutler , K.H. Wiltshire , B. Meyer , C. Moldaenke , C. Luring , M. Meyerhofer , U. P. Hansen and H. Dau (2002) A fluorometric method for the differentiation of algal populations in vivo and in situ. Photosynthesis Research 72, 3953.

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