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Egg volume, energy content and fatty acid profile of Maja brachydactyla (Crustacea: Brachyura: Majidae) during embryogenesis

  • Joana Figueiredo (a1) and Luís Narciso (a1)

Fatty-acid and energy content of Maja brachydactyla eggs at different developmental stages (recently spawned, half-developed and ready to hatch) were analysed in order to understand what is being consumed and produced during the embryonic development. Egg volume increased during development (34%, 0.187 to 0.285 mm3, N = 270) and was negatively correlated with egg energy and fatty-acid content (r = −0.80 and r = −0.46, respectively), which decreased through embryogenesis. The most consumed fatty acids were the PUFA (21.2 μg · mg dw−1), followed by the SFA (18.8 μg · mg dw−1) and MUFA (14.9 μg · mg dw−1). Palmitic (16:0), oleic (18:1n-9) and eicosapentaenoic (EPA, 20:5n-3) acids were preferentially consumed (13.14, 9.21 and 8.67 μg · mg dw−1, respectively). The fatty acid composition found in M. brachydactyla eggs reflected the habitat and omnivorous and detritivorous scanvenger diet of the adults, although the consumption of algae was more important than previously thought, at least in the area where these adults were captured. Pre-hatching eggs have a high PUFA content (64.5 μg · mg dw−1 or 46.3% of the egg fatty-acid content). We conclude that larvae of this species might need a diet rich in PUFA, particularly EPA and DHA, for successful development. From the culture perspective, live preys commonly used in aquaculture will likely require to be enriched with DHA.

Corresponding author
Correspondence should be addressed to: Joana Figueiredo, Laboratório Marítimo da Guia, Centro de Oceanografia, Faculdade de Ciências da Universidade de Lisboa, Avenida Nossa Senhora do Cabo, 939, 2750–374 CascaisPortugal email:
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M.O. Amsler and R.Y. George (1984) Seasonal variation in the biochemical composition of the embryos of Callinectes sapidus Rathbun. Journal of Crustacean Biology 4, 546553.

M. Andrés , A. Estévez and G. Rotllant (2007) Growth, survival and biochemical composition of spider crab Maja brachydactyla (Balss, 1922) (Decapoda: Majidae) larvae reared under different stocking densities, prey:larva ratios and diets. Aquaculture 273, 494502.

K. Anger (1995) The conquest of freshwater and land by marine crabs: adaptations in life-history patterns and larval bioenergetics. Journal of Experimental Marine Biology and Ecology 193, 119145.

K. Anger (1998) Patterns of growth and chemical composition in decapod crustacean larvae. Invertebrate Reproduction and Development 33, 159176.

C. Bernárdez , J. Freire and E. González-Gurriarán (2000) Feeding of the spider crab Maja squinado in rocky subtidal areas of the Ría de Arousa (north-west Spain). Journal of the Marine Biological Association of the United Kingdom 80, 95102.

E.G. Bligh and W.J. Dyer (1959) A rapid method of total lipids extraction and purification. Canadian Journal of Biochemistry and Physiology 37, 911917.

A. Clarke , J.H. Brown and L.J. Holmes (1990) The biochemical composition of eggs from Macrobrachium rosenbergii in relation to embryonic development. Comparative Biochemistry and Physiology—Part B 96, 505511.

J. Dalsgaard , M. St John , G. Kattner , D. Müller-Navarra and W. Hagen (2003) Fatty acid trophic markers in the pelagic marine environment. Advances in Marine Biology 46, 225340.

M.C. De Vries and R.B. Forward Jr (1991) Mechanisms of crustacean egg hatching: evidence for enzyme release by crab embryos. Marine Biology 110, 281291.

J.R. Ellis , S.I. Rogers and S.M. Freeman (2000) Demersal assemblages in the Irish Sea, St George's Channel and Bristol Channel. Estuarine, Coastal and Shelf Science 51, 299315.

E. González-Gurriarán , L. Fernández , J. Freire and R. Muiño (1998) Mating and role of seminal receptacles in the reproductive biology of the spider crab Maja squinado (Decapoda: Majidae). Journal of Experimental Marine Biology and Ecology 220, 269285.

M.H.A. Leme (2006) Seasonal changes in reproductive traits of the crab Sesarma rectum (Grapsoidea: Sesarmidae) on the northern coast of São Paulo State, Brazil. Journal of Crustacean Biology 26, 141147.

L.D. Metcalfe and A.A. Schmitz (1961) The rapid preparation of fatty acids esters for gass chromatography analysis. Analytical Chemistry 33, 363364.

L. Narciso and S. Morais (2001) Fatty acid profile of Palaemon serratum (Palaemonidae) eggs and larvae during embryonic and larval development using different live diets. Journal of Crustacean Biology 21, 566574.

V. Neumann (1998) A review of the Maja squinado (Crustacea: Decapoda: Brachyura) species-complex with a key to the eastern Atlantic and Mediterranean species of the genus. Journal of Natural History 32, 16671684.

C. Oh and R.G. Hartnoll (1999) Brood loss during incubation in Philocheras trispinosus (Decapoda) in Port Erin Bay, Isle of Man. Journal of Crustacean Biology 19, 467476.

S. Petersen and K. Anger (1997) Chemical and physiological changes during embryonic development of the spider crab, Hyas araneus L. (Decapoda: Majidae). Comparative Biochemistry and Physiology—Part B 117, 299306.

J.R. Rainuzzo , K.I. Reitan and Y. Olsen (1997) The significance of lipids at early stages of marine fish: a review. Aquaculture 155, 103116.

R. Rosa , R. Calado , L. Narciso and M.L. Nunes (2007) Embryogenesis of decapod crustaceans with different life history traits, feeding ecologies and habitats: a fatty acid approach. Marine Biology 151, 935947.

C. Scott , S. Kwasniewski , S. Falk-Petersen and J.R. Sargent (2002) Species differences, origins and functions of fatty acids in the wax esters and phospholipids of Calanus hyperboreus, C. glacialis and C. finmarchicus from Artic waters. Marine Ecology Progress Series 235, 127134.

T.F. Sørensen , G. Drillet , K. Engell-Sørensen , B.W. Hansen and H. Ramløv (2007) Production and biochemical composition of eggs from neritic calanoid copepods reared in large outdoor tanks (Limfjord, Denmark). Aquaculture 263, 8496.

P. Sorgeloos , P. Dhert and P. Candreva (2001) Use of the brine shrimp, Artemia spp., in marine fish larviculture. Aquaculture 200, 147159.

J.L. Staton and S.D. Sulkin (1991) Nutritional requirements and starvation resistance in larvae of the brachyuran crabs Sesarma cinereum (Bosc) and S. reticulatum (Say). Journal of Experimental Marine Biology and Ecology 152, 271284.

J.K. Volkman , S.M. Barrett , S.I. Blackburn , M.P. Mansour , E.L. Sikes and F. Gelin (1998) Microalgal biomarkers: a review of recent research developments. Organic Geochemistry 29, 11631179.

I.S. Wehrtmann and M. Graeve (1998) Lipid composition and utilization in developing eggs of two tropical marine caridean shrimps (Decapoda: Caridea: Alpheidae, Palaemonidae). Comparative Biochemistry and Physiology—Part B 121, 457463.

I.S. Wehrtmann and G. Kattner (1998) Changes in volume, biomass, and fatty acids of developing eggs in Nauticaris magellanica (Decapoda: Caridea): a latitudinal comparison. Journal of Crustacean Biology 18, 413422.

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Journal of the Marine Biological Association of the United Kingdom
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  • EISSN: 1469-7769
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