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DNA barcoding of commercially relevant marine fish species in Tunisian waters

Published online by Cambridge University Press:  28 July 2022

Sara M. Francisco Open the ORCID record for Sara M. Francisco [Opens in a new window] ,
Cristina S. Lima Open the ORCID record for Cristina S. Lima [Opens in a new window] ,
Inês Moreira Open the ORCID record for Inês Moreira [Opens in a new window] ,
Adel A. Basyouny Shahin Open the ORCID record for Adel A. Basyouny Shahin [Opens in a new window]  and
Abderraouf Ben Faleh Open the ORCID record for Abderraouf Ben Faleh [Opens in a new window]
Sara M. Francisco*
Affiliation:
MARE – Marine and Environmental Sciences Centre, ISPA – Instituto Universitário, Rua Jardim do Tabaco 34, 1149-041 Lisbon, Portugal
Cristina S. Lima
Affiliation:
MARE – Marine and Environmental Sciences Centre, ISPA – Instituto Universitário, Rua Jardim do Tabaco 34, 1149-041 Lisbon, Portugal
Inês Moreira
Affiliation:
MARE – Marine and Environmental Sciences Centre, ISPA – Instituto Universitário, Rua Jardim do Tabaco 34, 1149-041 Lisbon, Portugal
Adel A. Basyouny Shahin
Affiliation:
Department of Zoology, Faculty of Science, Minia University, El Minia, Egypt
Abderraouf Ben Faleh
Affiliation:
Laboratory of Ecology, Biology and Physiology of Aquatic Organisms (LR/18/ES/41), Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis, Tunisia
*
Author for correspondence: Sara M. Francisco, E-mail: sara_francisco@ispa.pt
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Abstract

DNA barcoding based on the cytochrome c oxidase subunit I (COI) gene in mitochondrial DNA has been adopted as a global biological identification system for animals due to its accuracy compared with other classical taxonomic methods. The objective of this study was to establish a reference library with generated barcodes. A total of 84 fish specimens belonging to 37 commercially important marine fish species, representing five orders, 14 families and 30 genera, were sampled along the Tunisian coast and barcoded for the first time, obtaining 637 bp sequences. The average Kimura 2-parameter (K2P) distances within species, genera and families were 0.52, 6.86 and 14.60%, respectively. The Maximum likelihood (ML) tree revealed distinct clusters in concurrence with the taxonomic status of the species. Our results confirmed the authentication of the barcode approach for the identification of the species examined and provide valuable information that would help ichthyologists to achieve better monitoring, conservation and management of fisheries in Tunisia.

Keywords

Cytochrome c oxidase subunit I (COI)DNA barcodingmarine fishmitochondrial DNATunisia
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 102 , Issue 3-4 , June 2022 , pp. 178 - 185
Copyright
Copyright © MARE – Marine and Environmental Sciences Centre, ISPA-Instituto Universitário, Rua Jardim do Tabaco 34, 1149-041 Lisbon, Portugal, 2022. Published by Cambridge University Press on behalf of Marine Biological Association of the United Kingdom.

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