Skip to main content Accessibility help
×
Home

Morphological and molecular characterization of a new species of Atlantic stalked barnacle (Scalpelliformes: Pollicipedidae) from the Cape Verde Islands

  • Javier Quinteiro (a1), Jorge Rodríguez-Castro (a1), Pedro López (a2), Luis F. López-Jurado (a3), Nieves González-Henríquez (a4) and Manuel Rey-Méndez (a1)...

Abstract

The taxonomy of pedunculate cirripedes belonging to the genus Pollicipes has essentially remained unchanged since Charles Darwin described them in his exhaustive work on the Cirripedia. This genus includes three species of stalked barnacles: Pollicipes pollicipes in the north-eastern Atlantic, P. polymerus in the north-eastern Pacific and P. elegans in the central-eastern Pacific. However, a population genetics analysis of P. pollicipes suggested the presence of a putative cryptic species collected from the Cape Verde Islands in the central-eastern Atlantic. This study examines the morphology of these genetically divergent specimens and compares them with that of representative Atlantic samples of the biogeographically closely related P. pollicipes and with the poorly described P. elegans. Molecular data, including mitochondrial COX1 and nuclear ribosomal interspaces sequences, were obtained for all species of the genus Pollicipes. Morphological distinctiveness, diagnostic characters, congruent divergence level and monophyletic clustering, at both nuclear and mitochondrial loci support the taxonomic status of this new species, Pollicipes darwini.

Copyright

Corresponding author

Correspondence should be addressed to:J. Quinteiro, Laboratorio de Sistemática Molecular (Unidad Asociada CSIC-IIM), Departamento Bioquímica e Bioloxía Molecular, CIBUS Universidade de Santiago de Compostela, 15782 Santiago de Compostela (A Coruña), Spain email: javier.quinteiro@usc.es

References

Hide All
Ballard, J.W.O. and Whitlock, M.C. (2004) The incomplete natural history of mitochondria. Molecular Ecology 13, 729744.
Barnes, M. (1996) Pedunculate cirripedes of the genus Pollicipes. Oceanography and Marine Biology: an Annual Review 34, 303394.
Buckridge, J.S. and Newman, W.A. (2006) A revision of the Iblidae and the stalked barnacles (Crustacea: Cirripedia: Thoracica), including new ordinal, familial and generic taxa, and two new species from New Zealand and Tasmanian waters. Zootaxa 1136, 138.
Chan, B.K.K., Tsang, L.M. and Chu, K.H. (2007a) Cryptic diversity of the Tetraclita squamosa complex (Crustacea: Cirripedia) in Asia: description of a new species from Singapore. Zoological Studies 46, 4656.
Chan, B.K.K., Tsang, L.M. and Chu, K.H. (2007b) Morphological and genetic differentiation of the acorn barnacle Tetraclita squamosa (Crustacea, Cirripedia) in East Asia and description of a new species of Tetraclita. Zoologica Scripta 36, 7991.
Costa, F.O., deWaard, J.R., Boutillier, J., Ratnasingham, S., Dooh, R.T. and Hebert, P.D. (2007) Biological identifications through DNA barcodes: the case of the Crustacea. Canadian Journal of Fisheries and Aquatic Sciences 64, 272295.
Darwin, C.R. (1851) A monograph on the subclass Cirripedia, with figures of all species. The Lepididae; or, pedunculated cirripides. London: The Ray Society.
Felsenstein, J. (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783791.
Fischer, M. and Steel, M. (2009) Sequence length bounds for resolving a deep phylogenetic divergence. Journal of Theoretical Biology 256, 247252.
Folmer, O., Black, M., Hoeh, W., Lutz, R. and Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3, 249299.
Foster, B.A. (1978) The marine fauna of New Zealand: barnacles (Cirripedia: Thoracica). Memoirs of the New Zealand Oceanographic Institute 69, 1160.
Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41, 9598.
Lavane, C., Preparata, G., Saccone, C. and Serio, G. (1984) A new method for calculating evolutionary substitution rates. Journal of Molecular Evolution 20, 8693.
Newman, W.A. (1987) Evolution of cirripedes and their major groups. In Southward, A.J. (ed.) Barnacle biology. Rotterdam: A.A. Balkema, pp. 342.
Posada, D. and Crandall, K.A. (1998) Modeltest: testing the model of DNA substitution. Bioinformatics 14, 817818.
Quinteiro, J., Rodríguez-Castro, J. and Rey-Méndez, M. (2007) Population genetic structure of the stalked barnacle Pollicipes pollicipes (Gmelin, 1789) in the northeastern Atlantic: influence of coastal currents and mesoscale hydrographic structures. Marine Biology 153, 4760.
Saitou, N. and Nei, M. (1987) The neighbor-joining method: A new method for reconstruction of phylogenetic trees. Molecular Biology and Evolution 4, 406425.
Southward, A.J. (2008) Barnacles. Shrewsbury: Field Studies Council (FSC).
Swofford, D.L. (2002) PAUP*. Phylogenetic analysis using parsimony (* and other methods). Sunderland, MA: Sinauer Associates.
Tamura, K. and Nei, M. (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution 10, 512526.
Tsang, L.M., Chan, B.K.K., Wu, T.H., Ng, W.C., Chatterjee, T., Williams, G.A. and Chu, K.H. (2008) Population differentiation in the barnacle Chthamalus malayensis: postglacial colonization and recent connectivity across the Pacific and Indian Oceans. Marine Ecology Progress Series 364, 107118.

Keywords

Morphological and molecular characterization of a new species of Atlantic stalked barnacle (Scalpelliformes: Pollicipedidae) from the Cape Verde Islands

  • Javier Quinteiro (a1), Jorge Rodríguez-Castro (a1), Pedro López (a2), Luis F. López-Jurado (a3), Nieves González-Henríquez (a4) and Manuel Rey-Méndez (a1)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed