Four species of Pisionidae (Polychaeta) are reported from Coiba National Park (Pacific, Panamá), one is a well known species of the area Pisionidens indica, one is a new species of Pisione and there are two new reports: Pisione galapagoensis and Pisione cf. papuensis. Pisione longispinulata sp. nov. possesses buccal aciculae distally elongated with a conspicuous notch, three compound chaetae per parapodium, with dense and long spines on margins, and a single pair of copulatory organs, each with modified ventral cirrus, distal spherical enlargement and distal papillae. The closest species is Pisione garciavaldecasasi, also from the Coiba National Park, although it lives in freshwater streams in the northern part of Coiba Island.