Skip to main content
×
Home
    • Aa
    • Aa

Ribosomal internal transcribed spacer regions are not suitable for intra- or inter-specific phylogeny reconstruction in haplosclerid sponges (Porifera: Demospongiae)

  • Niamh E. Redmond (a1) and Grace P. McCormack (a2)
Abstract

Sequences of the ribosomal internal transcribed spacer regions 1 and 2 (ITS-1 and ITS-2) were employed to investigate relationships between putatively very closely related species of marine haplosclerids and to investigate the species status of Haliclona cinerea. Results indicate that intra-genomic and intra-specific levels of diversity are equivalent, and sequences from multiple clones from a number of individuals of a single species could not be separated on phylogenetic trees. As a result, the ITS regions are not suitable markers for population level studies in marine haplosclerids. Sequences of these regions were highly species specific, and large differences were found between species. ITS sequences from three Callyspongia and three Haliclona species could not be aligned successfully and therefore this locus could not be used to investigate relationships between these putative close relatives. However, ITS sequences retrieved from one H. cinerea were very different from sequences generated from other H. cinerea individuals indicating that this species comprises more than one taxon.

Copyright
Corresponding author
Correspondence should be addressed to: G. McCormack, Molecular Evolution and Systematics Laboratory, MRI, Zoology Department, National University of Ireland, Galway, Ireland email: grace.mccormack@nuigalway.ie
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

J.S. Addis and K.J. Peterson (2005) Phylogenetic relationships of freshwater sponges (Porifera, Spongillina) inferred from analyses of 18S rDNA, COI mtDNA, and ITS-2 rDNA sequences. Zoologica Scripta 34, 549557.

B. Bentlage and G. Wörheide (2007) Low genetic structuring among Pericharax heteroraphis (Porifera: Calcarea) populations from the Great Barrier Reef (Australia), revealed by analysis of nrDNA and nuclear intron sequences. Coral Reefs 26, 807816.

D. Erpenbeck , G.P. McCormack , J.A.J. Breeuwer and R.W.M. van Soest (2004) Order level differences in the structure of partial LSU across demosponges (Porifera): new insights into an old taxon. Molecular Phylogenetics and Evolution 32, 388395.

B.J. Hackett , J. Gimnig , W. Guelbeogo , C. Costantini , L.L. Koekemoer , M. Coetzee , F.H. Collins and N.J. Besansky (2000) Ribosomal DNA internal transcribed spacer (ITS2) sequences differentiate Anopheles funestus and An. Rivulorum, and uncover a cryptic taxon. Insect Molecular Biology 9, 369374.

W.C. Jones (1987) Skeletal variation in embryo-containing specimens of Haliclona rosea (Bowerbank) from Anglesey, North Wales. In J. Vacelet and N. Boury-Esnault (eds) Taxonomy of Porifera. Berlin and Heidelberg: Springer-Verlag, pp. 101124.

N.P. Leo and S.C. Barker (2002) Intragenomic variation in ITS2 rDNA in the louse of humans, Pediculus humanus: ITS2 is not a suitable marker for population studies in this species. Insect Molecular Biology 11, 651657.

S.P. Leys and A.V. Ereskovsky (2006) Embryogenesis and larval differentiation in sponges. Canadian Journal of Zoology 84, 262287.

J.V. Lopez , C.L. Peterson , R. Willoughby , A.E. Wright , E. Enright , S. Zoladz , J.K. Reed and S.A. Pomponi (2002) Characterization of genetic markers for in vitro line identification of the marine sponge Axinella corrugata. Journal of Heredity 93, 2736.

G.P. McCormack , D. Erpenbeck and R.W.M. van Soest (2002) Major discrepancy between phylogenetic hypotheses based on molecular and morphological criteria within the Order Haplosclerida (Phylum Porifera: Class Demospongiae). Journal of Zoological Systematics and Evolutionary Research 40, 237240.

A. Mello , S. Ghignone , A. Vizzini , C. Sechi , P. Ruiu and P. Bonfante (2006) ITS primers for the identification of marketable boletes. Journal of Biotechnology 121, 318329.

S.A. Nichols (2005) An evaluation of support for order-level monophyly and interrelationships within the class Demospongiae using partial data from the large subunit rDNA and cytochrome oxidase subunit I. Molecular Phylogenetics and Evolution 34, 8196.

D.M. Odorico and D.J. Miller (1997) Variation in the ribosomal internal transcribed spacers and 5.8S rDNA among five species of Acropora (Cnidaria: Scleractina): patterns of variation consistent with reticulate evolution. Molecular Biology and Evolution 14, 465473.

N.E. Redmond , R.W.M. van Soest , M. Kelly , J. Raleigh , S.A.A. Travers and G.P. McCormack (2007) Reassessment of the classification of the Order Haplosclerida (Class Demospongiae, Phylum Porifera) using 18S rRNA gene sequence data. Molecular Phylogenetics and Evolution 43, 344352.

N.E. Redmond and G.P. McCormack (2008) Large expansion segments in 18S rDNA support a new sponge clade (Class Demospongiae, Order Haplosclerida). Molecular Phylogenetics and Evolution 47, 10901099.

S. Schmitt , U. Hentschel , S. Zea , T. Dandekar and M. Wolf (2005) ITS-2 and 18S gene phylogeny of Aplysiniidae (Verongida, Demospongiae). Journal of Molecular Evolution 60, 327336.

G. Wörheide , J.N.A. Hooper and M. Degnan (2002) Phylogeographic of western Pacific Leucetta ‘chagosensis’ (Porifera: Calcarea) from ribosomal DNA sequences: implications for population history and conservation of the Great Barrier Reef World Heritage Area (Australia). Molecular Ecology 11, 17531768.

G. Wörheide , S.A. Nichols and J. Goldberg (2004) Intragenomic variation of the rDNA internal transcribed spacers in sponges (Phylum Porifera): implications for phylogenetic studies. Molecular Phylogenetics and Evolution 33, 816830.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Journal of the Marine Biological Association of the United Kingdom
  • ISSN: 0025-3154
  • EISSN: 1469-7769
  • URL: /core/journals/journal-of-the-marine-biological-association-of-the-united-kingdom
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords:

Metrics

Full text views

Total number of HTML views: 2
Total number of PDF views: 4 *
Loading metrics...

Abstract views

Total abstract views: 72 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 23rd May 2017. This data will be updated every 24 hours.