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  • Journal of the Marine Biological Association of the United Kingdom, Volume 91, Issue 3
  • May 2011, pp. 727-733

The relationship between temperature, oxygen condition and embryo encapsulation in the marine gastropod Chorus giganteus

  • Juan M. Cancino (a1), José A. Gallardo (a2) and Antonio Brante (a1)
  • DOI:
  • Published online: 27 September 2010

Intracapsular oxygen availability is one of the main factors affecting embryo development of marine gastropod species with encapsulation. This is because the low solubility and diffusion rate of O2 in water, plus the low oxygen diffusion rate that the capsule wall presents, reduces oxygen inside capsules. In addition, temperature affects embryo development inside capsules through its effect on embryo metabolic rate and oxygen availability. In spite of both factors being highly correlated and that a synergic effect on embryo development may be expected, there are few studies evaluating temperature and intracapsular oxygen availability simultaneously. In this work we evaluated the role of the capsule wall of the marine gastropod Chorus giganteus as a barrier for oxygen diffusion and its interaction with temperature affecting intracapsular oxygen availability and embryonic development. For that, we cultivated capsules in seawater at three different temperatures, 9, 12 and 15°C, for a time to complete embryo development. Oxygen level was measured inside capsules with and without embryos, and outside capsules at all temperatures. The number of capsules successfully hatched at the end of the experiment, and early and late embryo mortality were recorded. Finally, we measured embryo metabolic rate at the three different temperatures assayed. We found that embryo mortality and abnormal morphological development were more frequent at higher temperatures. Intracapsular oxygen availability decreases at higher temperatures in capsules with and without embryos. These results may be explained by an increase in the total intracapsular embryo metabolic rate (per capsule) with temperature and an inadequate oxygen diffusion rate from seawater through the capsule wall and intracapsular fluid to the embryonic cells. Our findings suggest that encapsulation is constrained at high temperatures in C. giganetus affecting significantly its reproductive success. This may have important consequences in a scenario of global warming.

Corresponding author
Correspondence should be addressed to: J.M. Cancino, Departamento de Ecología Costera, Facultad de Ciencias, Universidad Católica de la Santísima Concepción, Casilla 297, Concepción, Chile email:
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S. Baker and R. Mann (1994) Description of metamorphic phases in the oyster Crassostrea virginica and effects of hypoxia on metamorphosis. Marine Ecology Progress Series 104, 9199.

A. Brante (2005) An alternative mechanism to reduce intracapsular hypoxia in ovicapsules of Fusititron oregonensis (Gastropoda). Marine Biology 149, 269274.

A. Brante , F. Viard and M. Fernández (2008) Does the intracapsular oxygen availability explain the developmental mode of encapsulated marine gastropods?: answers from the calyptraeid group. Marine Ecology Progress Series 368, 197207.

A. Brante , F. Viard and M. Fernández (2009) Limiting factors to encapsulation: the combined effects of dissolved protein and oxygen availability on embryonic growth and survival of species with contrasting feeding strategies. Journal of Experimental Biology 212, 22872295.

J.M. Cancino , J.A. Gallardo , F.A. Torres , G. Leiva and J.M. Navarro (2000) Effects of sessile Protozoa on intracapsular oxygen tension and embryonic shell calcification in the muricid Chorus giganteus. Marine Ecology Progress Series 200, 141148.

C. Chaffee and R. Strathmann (1984) Constraints on egg masses. I. Retarded development within thick egg masses. Journal of Experimental Marine Biology and Ecology 84, 7383.

E.R. Cronin and R.S. Seymour (2000) Respiration of the eggs of the giant cuttlefish Sepia apama. Marine Biology 136, 863870.

C. Cohen and R. Strathmann (1996) Embryos at the edge of tolerance: effects of environment and structure of egg masses on supply of oxygen to embryos. Biological Bulletin. Marine Biological Laboratory, Woods Hole 190, 815.

M.L. Crump (1996) Parental care among the Amphibia. In J.S. Rosenblatt and C.T. Snowdon (eds) Advances in the study of behavior. Amsterdam: Elsevier, pp. 109144.

J.T. Dick , S.E. Faloon and R.W. Elwood (1998) Active brood care in an amphipod: influences of embryonic development, temperature and oxygen. Animal Behaviour 56, 663672.

M. Fernández , C. Bock and H.O. Pörtner (2000) The cost of being a caring mother: the ignored factor in the reproduction of marine invertebrates. Ecology Letters 3, 487494.

M. Fernández , R. Calderón , J.M. Cancino and K. Jeno (2007) Effect of temperature on the development of encapsulated embryos of Concholepas concholepas along a latitudinal cline. Marine Ecology Progress Series 348, 229237.

C. Gallardo (1981) Posturas y estadios de eclosión del gastrópodo muricidae Chorus giganteus (Lesson, 1829). Studies on Neotropical Fauna and Environment 16, 3544.

K. Gonzalez and C. Gallardo (1999) Embryonic and larval development of the muricid snail Chorus giganteus (Lesson, 1829) with an assessment of the development nutrition sourse. Ophelia 51, 7792.

O. Hoegh-Guldberg and J.S. Pearse (1995) Temperature, food availability, and the development of marine invertebrate larvae. American Zoologist 35, 415425.

M.A. Lardies and M. Fernández (2002) Effect of oxygen availability in determining clutch size in Acanthina monodon. Marine Ecology Progress Series 239, 139146.

C.E. Lee and R.R. Strathmann (1998) Scaling of gelatinous clutches: effects of siblings' competition for oxygen on clutch size and parental investment per offspring. American Naturalist 151, 293310.

G. Lima and J. Pechenik (1985) The influence of temperature on growth rate and length of larval life of the gastropod, Crepidula plana. Journal of Experimental Marine Biology and Ecology 90, 5571.

A.L. Moran and A.H. Woods (2007) Oxygen in egg masses: interactive effects of temperature, age, and egg-mass morphology on oxygen supply to embryos. Journal of Experimental Biology 210, 722731.

J. Pechenik and G. Lima (1984) Relationship between growth, differentiation, and length of larval life for individually reared larvae of the marine gastropod, Crepidula fornicata. Biological Bulletin. Marine Biological Laboratory, Woods Hole 166, 537549.

H.O. Pörtner (2002) Climate variations and the physiological basis of temperature dependent biogeography: systemic to molecular hierarchy of thermal tolerance in animals. Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology 132, 739761.

R. Przeslawski (2004) A review of the effects of environmental stress on embryonic development within intertidal gastropod egg masses. Molluscan Research 24, 4363.

R. Roller and W. Stickle (1989) Temperature and salinity effects on the intracapsular development, metabolic rates, and survival to hatching of Thais haemastoma caniliculata (Gray) (Prosobranchia: Muricidae) under laboratory conditions. Journal of Experimental Marine Biology and Ecology 125, 235251.

R.R. Strathmann and C. Chaffee (1984) Constraints on egg masses. II Effect of spacing, size and number of eggs on ventilation of masses of embryos in jelly, adherent groups, or thinwalled capsules. Journal of Experimental Marine Biology and Ecology 84, 8593.

R.R. Strathmann and M.F. Strathmann (1982) The relationship between adult size and brooding in marine invertebrates. American Naturalist 119, 91101.

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Journal of the Marine Biological Association of the United Kingdom
  • ISSN: 0025-3154
  • EISSN: 1469-7769
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