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Morphological and Crystal-Chemical Features of Macro- and Microcalcifications of Human Aorta

Part of: Micrographia Collection

Published online by Cambridge University Press:  15 September 2021

Inna-Margaryta Radomychelski ,
Artem Piddubnyi ,
Sergey Danilchenko ,
Olena Maksymova ,
Yuliia Moskalenko  and
Roman Moskalenko Open the ORCID record for Roman Moskalenko [Opens in a new window]
Inna-Margaryta Radomychelski
Affiliation:
Department of Pathology, Sumy State University, Sumy, Ukraine
Artem Piddubnyi
Affiliation:
Department of Pathology, Sumy State University, Sumy, Ukraine Ukrainian-Swedish Research Center SUMEYA, Sumy, Ukraine
Sergey Danilchenko
Affiliation:
Institute of Applied Physics, National Academy of Sciences of Ukraine, Sumy, Ukraine
Olena Maksymova
Affiliation:
Department of Morphology, Sumy State University, Sumy, Ukraine
Yuliia Moskalenko
Affiliation:
Department of Oncology, Sumy State University, Sumy, Ukraine
Roman Moskalenko*
Affiliation:
Department of Pathology, Sumy State University, Sumy, Ukraine Ukrainian-Swedish Research Center SUMEYA, Sumy, Ukraine
*
*Corresponding author: Roman Moskalenko, E-mail: r.moskalenko@med.sumdu.edu.ua
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Abstract

Ectopic calcification or pathological biomineralization correlates with morbidity and mortality from cardiovascular diseases. Aortas with atherosclerotic lesions and biomineralization were selected for the study. Thirty samples of mineralized abdominal aortas (group M) were examined by histology. Depending on the calcifications size, samples were separated into group M1 (macroscopic calcifications) and M2 (microscopic calcifications). Each group consists of 15 samples. Calcification 2 mm or less were considered as microscopic, >2 mm—macroscopic. Thirty samples of aortic tissue without biomineralization (group C) were used as a control group. Aortic tissue was examined by macroscopic description, histology, histochemistry, immunohistochemistry (IHC), scanning electron microscopy (SEM) with microanalysis, and transmission electron microscopy (TEM). The results of IHC showed the involvement of OPN in the formation and development of pathological biomineralization, but the obvious role of OPN in the differentiation of macro- and microcalcifications of atherosclerotic aorta was not revealed. SEM with X-ray microanalysis confirmed that the biomineral part of the aortic samples of the M1 group consisted mainly of apatites, which correspond to previous studies. The Ca/P ratio was less in the M2 group than in the M1 group. It means that microcalcifications can be formed by more defective (immature) hydroxyapatite.

Keywords

atherosclerosisenergy-dispersive X-ray spectroscopyhistopathologyscanning electron microscopytransmission electron microscopy
Type
Micrographia
Information
Microscopy and Microanalysis , Volume 27 , Issue 6 , December 2021 , pp. 1539 - 1546
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

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