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An Improved Cryogen for Plunge Freezing

  • William F. Tivol (a1), Ariane Briegel (a1) and Grant J. Jensen (a1)

The use of an alkane mixture that remains liquid at 77 K to freeze specimens has advantages over the use of a pure alkane that is solid at 77 K. It was found that a mixture of methane and ethane did not give a cooling rate adequate to produce vitreous ice, but a mixture of propane and ethane did result in vitreous ice. Furthermore, the latter mixture produced less damage to specimens mounted on a very thin, fragile holey carbon substrate.

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A. Briegel , H.J. Ding , Z. Li , J. Werner , Z. Gitai , D.P. Dias , R.B. Jensen & G. Jensen (2008). Location and architecture of the Caulobacter crescentus chemoreceptor array. Mol Microbiol 69, 3041.

P. Brüggeller & E. Mayer (1980). Complete vitrification in pure liquid water and dilute aqueous solutions. Nature 288, 569571.

C.A. Griffith , P. Penteado , P. Rannou , R. Brown , V. Boudon , K.H. Baines , R. Clark , P. Drossart , B. Buratti , P. Nicholson , C.P. McKay , A. Coustenis , A. Negrao & R. Jaumann (2006). Evidence for a polar ethane cloud on Titan. Science 313, 16201622.

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Microscopy and Microanalysis
  • ISSN: 1431-9276
  • EISSN: 1435-8115
  • URL: /core/journals/microscopy-and-microanalysis
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