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Analysis of Cyclin E1 Functions in Porcine Preimplantation Embryonic Development by Fluorescence Microscopy

Published online by Cambridge University Press:  06 February 2017

Jing Guo ,
Kyung-Tae Shin  and
Xiang-Shun Cui
Jing Guo
Affiliation:
Department of Animal Sciences, Chungbuk National University, Chungbuk, Cheongju 361-763, Republic of Korea
Kyung-Tae Shin
Affiliation:
Department of Animal Sciences, Chungbuk National University, Chungbuk, Cheongju 361-763, Republic of Korea
Xiang-Shun Cui*
Affiliation:
Department of Animal Sciences, Chungbuk National University, Chungbuk, Cheongju 361-763, Republic of Korea
*
*Corresponding author. xscui@cbnu.ac.kr
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Abstract

Cyclin E1 (CCNE1) is a core component of cell cycle regulation that drives the transition into the S phase. CCNE1 plays critical roles in cell cycle, cell proliferation, and cellular functions. However, the function of CCNE1 in early embryonic development is limited. In the present study, the function and expression of Ccne1 in porcine early parthenotes were examined. Immunostaining experiments showed that CCNE1 localized in the nucleus, starting at the four-cell stage. Knockdown of Ccne1 by double-stranded RNA resulted in the failure of blastocyst formation and induced blastocyst apoptosis. Ccne1 depletion increased expression of the pro-apoptotic gene Bax, and decreased the expression of Oct4 and the rate of inner cell mass (ICM)/trophectoderm formation. The results indicated that CCNE1 affects blastocyst formation by inducing cell apoptosis and ICM formation during porcine embryonic development.

Keywords

Cyclin E1embryonic developmentapoptosispluripotencypigmicroRNA
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 23 , Issue 1 , February 2017 , pp. 69 - 76
Copyright
© Microscopy Society of America 2017 

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