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Combined Scanning Transmission Electron Microscopy Tilt- and Focal Series

  • Tim Dahmen (a1), Jean-Pierre Baudoin (a2), Andrew R. Lupini (a3), Christian Kübel (a3), Philipp Slusallek (a1) and Niels de Jonge (a2) (a4)...

In this study, a combined tilt- and focal series is proposed as a new recording scheme for high-angle annular dark-field scanning transmission electron microscopy (STEM) tomography. Three-dimensional (3D) data were acquired by mechanically tilting the specimen, and recording a through-focal series at each tilt direction. The sample was a whole-mount macrophage cell with embedded gold nanoparticles. The tilt–focal algebraic reconstruction technique (TF-ART) is introduced as a new algorithm to reconstruct tomograms from such combined tilt- and focal series. The feasibility of TF-ART was demonstrated by 3D reconstruction of the experimental 3D data. The results were compared with a conventional STEM tilt series of a similar sample. The combined tilt- and focal series led to smaller “missing wedge” artifacts, and a higher axial resolution than obtained for the STEM tilt series, thus improving on one of the main issues of tilt series-based electron tomography.

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Current address: La Timone Hospital and Medicine School, Marseille, France

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Microscopy and Microanalysis
  • ISSN: 1431-9276
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