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Dual Native MS and Cryo-EM Approach to Resolve Heteromeric Protein Assemblies and Subunit Stoichiometry

Published online by Cambridge University Press:  05 August 2019

Irina Novikova
Affiliation:
Environmental Molecular Sciences Laboratory, Richland, WA, USA.
Mowei Zhou
Affiliation:
Environmental Molecular Sciences Laboratory, Richland, WA, USA.
Jared Shaw
Affiliation:
Environmental Molecular Sciences Laboratory, Richland, WA, USA.
Hanjo Hellmann
Affiliation:
School of Biological Sciences, Washington State University, Pullman, WA, USA.
James E. Evans*
Affiliation:
Environmental Molecular Sciences Laboratory, Richland, WA, USA. School of Biological Sciences, Washington State University, Pullman, WA, USA.
*
*Corresponding author: james.evans@pnnl.gov
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Abstract

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Type
Cutting-Edge Microscopy in the Pacific Northwest
Copyright
Copyright © Microscopy Society of America 2019 

References

[1]Parra, M, Stahl, S and Hellmann, H, Cells 7(7) (2018) doi: 10.3390/cells7070084CrossRefGoogle Scholar
[2]Hsu, CC et al. , Food Nutr Res. 59 (2015), p. 25702. doi: 10.3402/fnr.v59.25702CrossRefGoogle Scholar
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[4]Brown, MJ and Beier, K in “Vitamin B6 Deficiency (Pyridoxine)”, (StatPearls, Treasure Island).Google Scholar
[5]Leuendorf, JE et al. , Mol Plant 3(5) (2010), p. 890. doi: 10.1093/mp/ssq041.CrossRefGoogle Scholar
[6]Rodrigues, MJ et al. , Nat Chem Biol 13(3) (2017), p. 290. doi: 10.1038/nchembio.2273.CrossRefGoogle Scholar
[7]Novikova, IV et al. , Advanced Structural and Chemical Imaging 4 (2018), p. 2198. doi: 10.1186/s40679-018-0062-9CrossRefGoogle Scholar
[8]A portion of this research was supported by NIH grant U24GM129547 and performed at the PNCC at OHSU and accessed through EMSL (grid.436923.9), a DOE Office of Science User Facility sponsored by the Office of Biological and Environmental Research. Additional support was provided by DOE-BER project FWP 66382 and EMSL Strategic Science Area project 50432.Google Scholar
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Dual Native MS and Cryo-EM Approach to Resolve Heteromeric Protein Assemblies and Subunit Stoichiometry
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