Hostname: page-component-76fb5796d-9pm4c Total loading time: 0 Render date: 2024-04-25T07:10:54.564Z Has data issue: false hasContentIssue false

High-resolution Imaging of Reconstituted Protein-DNA Complexes Using Phase Plate Electron Cryo Microscopy

Published online by Cambridge University Press:  25 July 2016

Sara Sandin
Affiliation:
School of Biological Sciences, Nanyang Technological University, Singapore NTU Institute of Structural Biology, Nanyang Technological University, Singapore
Radostin Danev
Affiliation:
Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
Juergen M. Plitzko
Affiliation:
Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
Eugene Chua Yue Dao
Affiliation:
School of Biological Sciences, Nanyang Technological University, Singapore NTU Institute of Structural Biology, Nanyang Technological University, Singapore
Vinod Kumar Vogirala
Affiliation:
School of Biological Sciences, Nanyang Technological University, Singapore NTU Institute of Structural Biology, Nanyang Technological University, Singapore
Andrew See Weng Wong
Affiliation:
School of Biological Sciences, Nanyang Technological University, Singapore NTU Institute of Structural Biology, Nanyang Technological University, Singapore

Abstract

Image of the first page of this content. For PDF version, please use the ‘Save PDF’ preceeding this image.'
Type
Abstract
Copyright
© Microscopy Society of America 2016 

References

References:

[1] Luger, K, Dechassa, ML & Tremethick, DJ Nat Rev Mol Cell Biol. 13(7 (2012). p. 436447.Google Scholar
[2] Danev, R, et al, Proc Natl Acad Sci U S A. 111(44 (2014). p. 1563515640.Google Scholar
[3] Khoshouei, M, et al, Nat Commun. 7 (2016). p. 10534.Google Scholar
[4] S Sandin, ECY Dao, VK Vogirala and ASW Wong acknowledge funding from Nanyang Technological University and Singapore Ministry of Education Academic Research Fund Tier 3 [MOE2012-T3-1-001], lead-PI D Rhodes. We thank L Nordenskiold’s group for their kind contribution of histones used in this study. We thank M Khoshouei, W Baumeister, C Davey and SHW Scheres for help and advice.Google Scholar