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Large Area SEM Backscatter Imaging of GABA Post-Embedding Immunogold, Web-Based Crowdsourcing Segmentation and Local Image Processing for Rigorous Quantification

Published online by Cambridge University Press:  05 August 2019

Cheryl Clarkson ,
Christopher T. Zugates ,
Christine A. Brantner ,
Matthias Rust  and
Anastas Popratiloff
Cheryl Clarkson*
Affiliation:
George Washington University, Nanofabrication and Imaging Center, Washington, DC, USA.
Christopher T. Zugates
Affiliation:
arivis AG, Washington, DC, USA.
Christine A. Brantner
Affiliation:
George Washington University, Nanofabrication and Imaging Center, Washington, DC, USA.
Matthias Rust
Affiliation:
arivis AG, Rostock, Germany.
Anastas Popratiloff
Affiliation:
George Washington University, Nanofabrication and Imaging Center, Washington, DC, USA.
*
*Corresponding author: cclarkson12@gwu.edu

Abstract

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Type
From Images to Insights: Working with Large Data in Cell Biological Imaging
Information
Microscopy and Microanalysis , Volume 25 , Supplement S2 , August 2019 , pp. 1392 - 1393
Copyright
Copyright © Microscopy Society of America 2019 

References

[1]Clarkson, C, Antunes, FM and Rubio, ME, J. Neurosci. 36 (2016), p.10214.Google Scholar
[2]Otsu, N, IEE Transaction on systems, Man, and cybernetics, SMC-9 (1979), p. 62.Google Scholar