Skip to main content
×
Home
    • Aa
    • Aa
  • Get access
    Check if you have access via personal or institutional login
  • Cited by 9
  • Cited by
    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.

    Spradling, Kyle Uribe, Brittany Okhunov, Zhamshid Hofmann, Martin del Junco, Michael Hwang, Christina Gruber, Caden Youssef, Ramy F. and Landman, Jaime 2015. Evaluation of Ignition and Burn Risk Associated with Contemporary Fiberoptic and Distal Sensor Endoscopic Technology. Journal of Endourology, Vol. 29, Issue. 9, p. 1076.


    Cole, Richard 2014. Live-cell imaging. Cell Adhesion & Migration, Vol. 8, Issue. 5, p. 452.


    Sarau, George Heilmann, Martin Latzel, Michael and Christiansen, Silke 2014. Disentangling the effects of nanoscale structural variations on the light emission wavelength of single nano-emitters: InGaN/GaN multiquantum well nano-LEDs for a case study. Nanoscale, Vol. 6, Issue. 20, p. 11953.


    McDonald, Alison Harris, John MacMillan, Debbi Dempster, John and McConnell, Gail 2012. Light-induced Ca^2+ transients observed in widefield epi-fluorescence microscopy of excitable cells. Biomedical Optics Express, Vol. 3, Issue. 6, p. 1266.


    Brydegaard, Mikkel Merdasa, Aboma Jayaweera, Hiran Ålebring, Jens and Svanberg, Sune 2011. Versatile multispectral microscope based on light emitting diodes. Review of Scientific Instruments, Vol. 82, Issue. 12, p. 123106.


    Cole, Richard W Jinadasa, Tushare and Brown, Claire M 2011. Measuring and interpreting point spread functions to determine confocal microscope resolution and ensure quality control. Nature Protocols, Vol. 6, Issue. 12, p. 1929.


    Li, Lihong Zhang, Shanqing and Zhao, Huijun 2011. A low cost universal photoelectrochemical detector for organic compounds based on photoelectrocatalytic oxidation at a nanostructured TiO2 photoanode. Journal of Electroanalytical Chemistry, Vol. 656, Issue. 1-2, p. 211.


    Piasecki, Tomasz Breadmore, Michael C. and Macka, Mirek 2010. White LEDs as broad spectrum light sources for spectrophotometry: Demonstration in the visible spectrum range in a diode-array spectrophotometric detector. ELECTROPHORESIS, Vol. 31, Issue. 22, p. 3737.


    De, Arijit Kumar and Goswami, Debabrata 2009. A Systematic Study on Fluorescence Enhancement under Single-photon Pulsed Illumination. Journal of Fluorescence, Vol. 19, Issue. 5, p. 931.


    ×

Light-Emitting Diodes Are Better Illumination Sources for Biological Microscopy than Conventional Sources

  • Richard W. Cole (a1) and James N. Turner (a1)
  • DOI: http://dx.doi.org/10.1017/S1431927608080288
  • Published online: 01 March 2008
Abstract
Abstract

Light-emitting diodes (LEDs) can be easily and inexpensively integrated into modern light microscopes. There are numerous advantages of LEDs as illumination sources; most notably, they provide brightness and spectral control. We demonstrate that for transmitted light imaging, an LED can replace the traditional tungsten filament bulb while offering longer life; no color temperature change with intensity change; reduced emission in the infrared region, which is important for live cell imaging; and reduced cost of ownership. We show a direct substitution of the typical tungsten bulb with a commercially available LED and demonstrated the color stability by imaging a histology section over a wide range of light intensities. For fluorescent imaging, where the typical illumination sources are mercury or xenon lamps, we demonstrate that LEDs offer advantages of providing a longer lifespan, having a more constant intensity output over time, more homogeneous illumination, and significantly lower photon dose. Our LED equipped system was used to image and deconvolve dual fluorescently labeled cells, as well as image cells undergoing mitosis expressing green fluorescent protein–histone 2B complex. The timing of the stages of mitosis is well established as an indicator of cell viability.

Copyright
Corresponding author
Corresponding author. E-mail: rcole@wadsworth.org
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

J.M. Benavides & R.M. Webb (2005). Optical characterization of ultrabright LEDs. Appl Optics 44, 40004003.

V. Bormuth , J. Howard & E. Schaffer (2007). LED illumination for video-enhanced DIC imaging of single microtubules. J Microsc 226, 15.

I.A. Eltoum & J. Roberson (2007). Impact of HPV testing, HPV vaccine development, and changing screening frequency on national Pap test volume: Projections from the National Health Interview Survey. Cancer 111, 3440.

C. Moser , T. Mayr , L. Klimant , C.G. Morgan , A.C. Mitchell , T. Heinlein , A. Biebricher , P. Schluter , C.M. Roth , D.P. Herten , J. Wolfrum , M. Heilemann , C. Muller , P. Tinnefeld & M. Sauer (2006). Filter cubes with built-in ultrabright light-emitting diodes as exchangeable excitation light sources in fluorescence microscopy. J Microsc 222, 135140.

T. Nishigaki , D.C. Wood , K. Shiba , S.A. Baba & A. Darszon (2006). Stroboscopic illumination using light-emitting diodes reduces phototoxicity in fluorescence cell imaging. Biotech 41, 191197.

C.L. Rieder & R.W. Cole (1998). Entry into mitosis in vertebrate somatic cells is guarded by a chromosome damage checkpoint that reverses the cell cycle when triggered during early but not late prophase. J Cell Bio 142, 10131022.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Microscopy and Microanalysis
  • ISSN: 1431-9276
  • EISSN: 1435-8115
  • URL: /core/journals/microscopy-and-microanalysis
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords: