Hostname: page-component-76fb5796d-r6qrq Total loading time: 0 Render date: 2024-04-25T11:03:48.373Z Has data issue: false hasContentIssue false
  • English
  • Français

New Viruses from Lacerta monticola (Serra da Estrela, Portugal): Further Evidence for a New Group of Nucleo-Cytoplasmic Large Deoxyriboviruses

Published online by Cambridge University Press:  08 December 2010

António Pedro Alves de Matos ,
Maria Filomena Alcobia da Silva Trabucho Caeiro ,
Tibor Papp ,
Bruno André da Cunha Almeida Matos ,
Ana Cristina Lacerda Correia  and
Rachel E. Marschang
António Pedro Alves de Matos*
Affiliation:
Anatomic Pathology Department, Curry Cabral Hospital, R. da Beneficência 8, 1069-166 Lisboa, Portugal CESAM—Centre for Environmental and Marine Studies. Aveiro University, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal
Maria Filomena Alcobia da Silva Trabucho Caeiro
Affiliation:
CESAM—Centre for Environmental and Marine Studies. Aveiro University, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal University of Lisbon, Faculty of Sciences, Department of Plant Biology, Campo Grande, 1749-016 Lisbon, Portugal
Tibor Papp
Affiliation:
Institut für Umwelt und Tierhygiene, Hohenheim University, Garbenstr. 30, 70599 Stuttgart, Germany
Bruno André da Cunha Almeida Matos
Affiliation:
Anatomic Pathology Department, Curry Cabral Hospital, R. da Beneficência 8, 1069-166 Lisboa, Portugal
Ana Cristina Lacerda Correia
Affiliation:
Anatomic Pathology Department, Curry Cabral Hospital, R. da Beneficência 8, 1069-166 Lisboa, Portugal
Rachel E. Marschang
Affiliation:
Institut für Umwelt und Tierhygiene, Hohenheim University, Garbenstr. 30, 70599 Stuttgart, Germany
*
Corresponding author. E-mail: apamatos@kanguru.pt
Get access

Abstract

Lizard erythrocytic viruses (LEVs) have previously been described in Lacerta monticola from Serra da Estrela, Portugal. Like other known erythrocytic viruses of heterothermic vertebrates, these viruses have never been adapted to cell cultures and remain uncharacterized at the molecular level. In this study, we made attempts to adapt the virus to cell cultures that resulted instead in the isolation of a previously undetected Ranavirus closely related to FV3. The Ranavirus was subsequently detected by polymerase chain reaction (PCR) in the blood of infected lizards using primers for a conserved portion of the Ranavirus major capsid protein gene. Electron microscopic study of the new Ranavirus disclosed, among other features, the presence of intranuclear viruses that may be related to an unrecognized intranuclear morphogenetic process. Attempts to detect by PCR a portion of the DNA polymerase gene of the LEV in infected lizard blood were successful. The recovered sequence had 65.2/69.4% nt/aa% homology with a previously detected sequence from a snake erythrocytic virus from Florida, which is ultrastructurally different from the studied LEV. These results further support the hypothesis that erythrocytic viruses are related to one another and may represent a new group of nucleo-cytoplasmic large deoxyriboviruses.

Keywords

lizard erythrocytic virusRanavirusLacerta monticolavirus isolationPCR
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 17 , Issue 1 , February 2011 , pp. 101 - 108
Copyright
Copyright © Microscopy Society of America 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Alves de Matos, A.P. & Paperna, I. (1993). Ultrastructural study of Pirhemocyton virus in lizard erythrocytes. Ann Parasitol Hum Comp 68, 24–33.CrossRefGoogle Scholar
Alves de Matos, A.P., Paperna, I. & Crespo, E. (2002). Experimental Infection of lacertids with lizard erythrocytic viruses. Intervirology 45, 150–159.Google Scholar
Bonfield, J.K., Smith, K.F. & Staden, R. (1995). A new DNA sequence assembly program. Nucleic Acids Res 24, 4992–4999.Google Scholar
Chinchar, V.G., Hyatt, A., Miyazaki, T. & Williams, T. (2009). Family Iridoviridae: Poor viral relations no longer. Curr Top Microbiol Immunol 328, 123–170.Google ScholarPubMed
Devauchelle, G., Stoltz, D.B. & Darcy-Tripier, F. (1985). Comparative ultrastructure of Iridoviridae. Curr Top Microbiol Immunol 116, 1–21.Google ScholarPubMed
Felsenstein, J. (1989). PHYLIP—Phylogeny Inference Package. Cladistics 5, 164–166.Google Scholar
Gray, M.J., Miller, D.L. & Hoverman, J.T. (2009). Ecology and pathology of amphibian ranaviruses. Dis Aquat Organ 87, 243–66.CrossRefGoogle ScholarPubMed
Gruia-Gray, J., Petric, M. & Desser, S.S. (1989). Ultrastructural, biochemical, and biological properties of an erythrocytic virus of frogs from Ontario, Canada. In Viruses of Lower Vertebrates, Ahne, W. & Kurstak, E. (Eds.), pp. 69–78. Berlin: Springer-Verlag.Google Scholar
Gruia-Gray, J., Ringuette, M. & Desser, S.S. (1992). Cytoplasmic localization of the DNA virus frog erythrocytic virus. Intervirology 33, 159–164.Google Scholar
Hall, T.A. (1999). BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 41, 95–98.Google Scholar
Hanson, L.A., Rudis, M.R., Vasquez-Lee, M. & Montgomery, R.D. (2006). A broadly applicable method to characterize large DNA viruses and adenoviruses based on the DNA polymerase gene. Virology J 3, 28–37.CrossRefGoogle ScholarPubMed
Hyatt, A.D., Williamson, M., Coupar, B.E., Middleton, D., Hengstberger, S.G., Gould, A.R., Selleck, P., Wise, T.G., Kattenbelt, J., Cunningham, A.A. & Lee, J. (2002). First identification of a ranavirus from green pythons (Chondropython viridis). J Wildl Dis 38, 239–252.Google Scholar
Jancovich, J.K., Bremont, M., Touchman, J.W. & Jacobs, B.L. (2010). Evidence for multiple recent host species shifts among the Ranaviruses (family Iridoviridae). J Virol 84, 2636–2647.Google Scholar
Johnsrude, J.D., Raskin, R.E., Hoge, A.Y.A. & Erdos, G.W. (1997). Intraerythrocytic inclusions associated with iridoviral infection in a fer de lance (Bothrops moojeni) snake. Vet Pathol 34, 235–238.Google Scholar
Johnston, M.R.L. (1975). Distribution of Pirhemocyton Chatton and Blanc and other, possibly related, infections of poikilotherms. J Protozool 22, 529–535.Google Scholar
Just, F., Essbauer, S., Ahne, W. & Blahak, S. (2001). Ocurrence of an invertebrate iridescent-like virus (Iridoviridae) in reptiles. J Vet Med B Infect Dis Vet Public Health 48, 685–694.Google Scholar
Kelly, D.C. & Atkinson, M.A. (1975). FV3 replication: Electron microscopic observations on the terminal stages of infection in chronically infected cell cultures. J Gen Virol 28, 391–407.Google Scholar
Mao, J., Hedrick, R.P. & Chinchar, G. (1997). Molecular characterization, sequence analysis and taxonomic position of newly isolated fish iridoviruses. Virology 229, 212–220.CrossRefGoogle ScholarPubMed
Marschang, R.E., Braun, S. & Becher, P. (2005). Isolation of a ranavirus from a gecko (Uroplatus fimbriatus). J Zoo Wildl Med 36, 295–300.CrossRefGoogle ScholarPubMed
Paperna, I. & Alves de Matos, A.P. (1993). Erythrocytic viral infections of lizards and frogs: New hosts, geographical locations and description of the infection process. Ann Parasitol Hum Comp 68, 11–23.Google Scholar
Schetter, C., Grünemann, B., Hölker, I. & Doerfler, W. (1993). Patterns of frog virus 3 DNA methylation and DNA methyltransferase activity in nuclei of infected cells. J Virol 67, 6973–6978.Google Scholar
Telford, S.R. & Jacobson, E.R. (1993). Lizard erythrocytic virus in east African chameleons. J Wildl Dis 29, 57–63.Google Scholar
Weinmann, N., Papp, T., Alves de Matos, A.P., Teifke, J.P. & Marschang, R.E. (2007). Experimental infection of crickets (Gryllus bimaculatus) with an invertebrate iridovirus isolated from a high-casqued chameleon (Chamaeleo hoehnelii). J Vet Diagn Invest 19, 674–679.Google Scholar
Wellehan, J.F. Jr., Strik, N.I., Stacy, B.A., Childress, A.L., Jacobson, E.R. & Telford, S.R. Jr. (2008). Characterization of an erythrocytic virus in the family Iridoviridae from a peninsula ribbon snake (Thamnophis sauritus sackenii). Vet Microbiol 131, 115–122.Google Scholar
Whittington, R.J., Becker, J.A. & Dennis, M.M. (2010). Iridovirus infections in finfish—Critical review with emphasis on ranaviruses. J Fish Dis 33, 95–122.Google Scholar
Williams, T., Barbosa-Solomieu, V. & Chinchar, V.G. (2005). A decade of advances in iridovirus research. Adv Virus Res 65, 173–248.Google Scholar
Yutin, N., Wolf, Y.I., Raoult, D. & Koonin, E.V. (2009). Eukaryotic large nucleo-cytoplasmic DNA viruses: Clusters of orthologous genes and reconstruction of viral genome evolution. Virology J 6, 223–235.Google Scholar