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Onset of Appearance and Potential Significance of Telocytes in the Developing Fetal Lung

  • Mahmoud Awad (a1), Wafaa Gaber (a2) and Dalia Ibrahim (a1)


CD34, vimentin, and vascular endothelial growth factor immunohistochemical analysis and electron microscopic tools were employed to record the initial appearance of telocytes (TCs) and stage-by-stage variations in TC localizations in the developing rabbit lung. TCs could not be identified in the primitive embryonic lung until day 18 of gestation. In the pseudoglandular lung, CD34+ TCs had been recorded under the cartilage of the main bronchus, in the wall of large-sized pulmonary vessels and large epithelial tubes. In the canalicular phase, TCs could be demonstrated in the smooth muscle layer of the bronchioles including the terminal ones. The strength of CD34 immunoreactive signals had been amplified by age until the day of parturition. Ultrastructurally, TCs consisted of a tiny body and exceptionally long telopodes (Tps). The Tp consisted of alternating thin segments (podomers) and dilated ones (podoms). The Tp sometimes branched with a dichotomous pattern. TCs interconnected in a network either by homocellular junctions with neighboring TCs or by heterocellular junctions with smooth muscle cells and alveolar cells. Collectively, early detection of TCs in pulmonary vessels suggests a potential role for TCs in their angiogenesis. For the lung tissue, TCs seem to be involved in the regulation of lung histogenesis.


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*Author for correspondence: D. Ibrahim, E-mail:;


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Abd-Elhafeez, HH & Soliman, SA (2017). New description of telocyte sheaths in the bovine uterine tube: An immunohistochemical and scanning microscopic study. Cells Tissues Organs 203(5), 295315.
Bani, D, Formigli, L, Gherghiceanu, M & Faussone-Pellegrini, MS (2010). Telocytes as supporting cells for myocardial tissue organization in developing and adult heart. J Cell Mol Med 14(10), 25312538.
Cantarero, CL, Luesma Bartolome, MJ & Junquera Escribano, C (2011). Identification of telocytes in the lamina propria of rat duodenum: Transmission electron microscopy. J Cell Mol Med 15(1), 2630.
Chang, Y, Li, C, Lu, Z, Li, H & Guo, Z (2015). Multiple immunophenotypes of cardiac telocytes. Exp Cell Res 338(2), 239244.
Chen, X, Zheng, Y, Manole, CG, Wang, X & Wang, Q (2013). Telocytes in human oesophagus. J Cell Mol Med 17(11), 15061512.
Chinoy, MR (2003). Lung growth and development. Front Biosci 8, d392d415.
Deng, W (2010). Mesenchymal stem cells express c-kit. Circ Res 107(10), e17, Author reply e18.
Eidenschink, L, DiZerega, G, Rodgers, K, Barlet, M, Wells, D & Loken, M (2012). Basal levels of CD34 positive cells in peripheral blood differ between individuals and are stable for 18 months. Cytometry B Clin Cytom 82, 1825.
Faussone-Pellegrini, MS & Gherghiceanu, M (2016). Telocyte's contacts. Semin Cell Dev Biol 55, 38.
Faussone Pellegrini, MS & Popescu, LM (2011). Telocytes. Biomol Concepts 2(6), 481489.
Gherghiceanu, M, Hinescu, ME, Andrei, F, Mandache, E, Macarie, CE, Faussone-Pellegrini, MS & Popescu, LM (2008). Interstitial Cajal-like cells (ICLC) in myocardial sleeves of human pulmonary veins. J Cell Mol Med 12(5A), 17771781.
Gherghiceanu, M & Popescu, LM (2011). Heterocellular communication in the heart: Electron tomography of telocyte-myocyte junctions. J Cell Mol Med 15(4), 10051011.
Hinescu, ME, Gherghiceanu, M, Suciu, L & Popescu, LM (2011). Telocytes in pleura: Two- and three-dimensional imaging by transmission electron microscopy. Cell Tissue Res 343(2), 389397.
Hinescu, ME, Popescu, LM, Gherghiceanu, M & Faussone-Pellegrini, MS (2008). Interstitial Cajal-like cells in rat mesentery: An ultrastructural and immunohistochemical approach. J Cell Mol Med 12(1), 260270.
Hussein, MW & Mokhtar, DM (2018). The roles of telocytes in lung development and angiogenesis: An immunohistochemical, ultrastructural, scanning electron microscopy and morphometrical study. Developmental Biology 15, 443(2), 137152.
Ingber, DE (2006). Cellular mechanotransduction: Putting all the pieces together again. FASEB J 20(7), 811827.
Karnak, I, Muftuoglu, S, Cakar, N & Tanyel, FC (1999). Organ growth and lung maturation in rabbit fetuses. Res Exp Med (Berl) 198(5), 277287.
Kostin, S & Popescu, LM (2009). A distinct type of cell in myocardium: Interstitial Cajal-like cells (ICLCs). J Cell Mol Med 13(2), 295308.
Mandache, E, Popescu, LM & Gherghiceanu, M (2007). Myocardial interstitial Cajal-like cells (ICLC) and their nanostructural relationships with intercalated discs: Shed vesicles as intermediates. J Cell Mol Med 11(5), 11751184.
Mihalcea, CE, Morosanu, AM, Murarasu, D, Puiu, L, Cinca, S, Voinea, SC & Mirancea, N (2015). Particular molecular and ultrastructural aspects in invasive mammary carcinoma. Rom J Morphol Embryol 56(4), 13711381.
Nicolescu, MI & Popescu, LM (2012). Telocytes in the interstitium of human exocrine pancreas: Ultrastructural evidence. Pancreas 41(6), 949956.
Popescu, LM, Ciontea, SM & Cretoiu, D (2007). Interstitial Cajal-like cells in human uterus and fallopian tube. Ann N Y Acad Sci 1101, 139165.
Popescu, LM & Faussone-Pellegrini, MS (2010). TELOCYTES - A case of serendipity: The winding way from interstitial cells of Cajal (ICC), via interstitial Cajal-like cells (ICLC) to TELOCYTES. J Cell Mol Med 14(4), 729740.
Popescu, LM, Gherghiceanu, M, Mandache, E & Cretoiu, D (2006). Caveolae in smooth muscles: Nanocontacts. J Cell Mol Med 10(4), 960990.
Popescu, LM, Gherghiceanu, M, Manole, CG & Faussone-Pellegrini, MS (2009). Cardiac renewing: Interstitial Cajal-like cells nurse cardiomyocyte progenitors in epicardial stem cell niches. J Cell Mol Med 13(5), 866886.
Popescu, LM, Gherghiceanu, M, Suciu, LC, Manole, CG & Hinescu, ME (2011). Telocytes and putative stem cells in the lungs: Electron microscopy, electron tomography and laser scanning microscopy. Cell Tissue Res 345(3), 391403.
Popescu, LM, Manole, CG, Gherghiceanu, M, Ardelean, A, Nicolescu, MI, Hinescu, ME & Kostin, S (2010). Telocytes in human epicardium. J Cell Mol Med 14(8), 20852093.
Radu, E, Regalia, T, Ceafalan, L, Andrei, F, Cretoiu, D & Popescu, LM (2005). Cajal-type cells from human mammary gland stroma: Phenotype characteristics in cell culture. J Cell Mol Med 9(3), 748752.
Reynolds, ES (1963). The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. Cell Biol 17, 208212.
Satelli, A & Li, S (2011). Vimentin in cancer and its potential as a molecular target for cancer therapy. Cell Mol Life Sci 68(18), 30333046.
Shi, L, Dong, N, Chen, C & Wang, X (2016). Potential roles of telocytes in lung diseases. Semin Cell Dev Biol 55, 3139.
Sidney, LE, Branch, MJ, Dunphy, SE, Dua, HS & Hopkinson, A (2014). Concise review: Evidence for CD34 as a common marker for diverse progenitors. Stem Cells 32(6), 13801389.
Song, D, Cretoiu, D, Cretoiu, SM & Wang, X (2016). Telocytes and lung disease. Histol Histopathol 31(12), 13031314.
Suciu, L, Popescu, LM, Gherghiceanu, M, Regalia, T, Nicolescu, MI, Hinescu, ME & Faussone-Pellegrini, MS (2010). Telocytes in human term placenta: Morphology and phenotype. Cells Tissues Organs 192(5), 325339.
Urban, L, Miko, M, Kajanova, M, Bozikova, S, Mrazova, H & Varga, I (2016). Telocytes (interstitial Cajal-like cells) in human fallopian tubes. Bratisl Lek Listy 117(5), 263267.
Wang, J, Jin, M, Ma, WH, Zhu, Z & Wang, X (2016). The history of telocyte discovery and understanding. Adv Exp Med Biol 913, 121.
Warburton, D, El-Hashash, A, Carraro, G, Tiozzo, C, Sala, F, Rogers, O, De Langhe, S, Kemp, PJ, Riccardi, D, Torday, Jet al. (2010). Lung organogenesis. Curr Top Dev Biol 90, 73158.
Weaver, M, Batts, L & Hogan, BL (2003). Tissue interactions pattern the mesenchyme of the embryonic mouse lung. Dev Biol 258(1), 169184.
Zheng, Y, Bai, C & Wang, X (2012 a). Potential significance of telocytes in the pathogenesis of lung diseases. Expert Rev Respir Med 6(1), 4549.
Zheng, Y, Bai, C & Wang, X (2012 b). Telocyte morphologies and potential roles in diseases. J Cell Physiol 227(6), 23112317.
Zheng, Y, Chen, X, Qian, M, Zhang, M, Zhang, D, Bai, C, Wang, Q & Wang, X (2014). Human lung telocytes could promote the proliferation and angiogenesis of human pulmonary microvascular endothelial cells in vitro. Mol Cell Ther 2, 3.
Zheng, Y, Li, H, Manole, CG, Sun, A, Ge, J & Wang, X (2011). Telocytes in trachea and lungs. J Cell Mol Med 15(10), 22622268.
Zheng, Y, Zhang, M, Qian, M, Wang, L, Cismasiu, VB, Bai, C, Popescu, LM & Wang, X (2013). Genetic comparison of mouse lung telocytes with mesenchymal stem cells and fibroblasts. J Cell Mol Med 17(4), 567577.


Onset of Appearance and Potential Significance of Telocytes in the Developing Fetal Lung

  • Mahmoud Awad (a1), Wafaa Gaber (a2) and Dalia Ibrahim (a1)


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