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Routine collection of 10,000 direct detector movies a day using Leginon

Published online by Cambridge University Press:  30 July 2021

Huihui Kuang
Affiliation:
New York Structural Biology Center, United States
Kashyap Maruthi
Affiliation:
New York Structural Biology Center, United States
Anchi Cheng
Affiliation:
NanoImaging Services, San Diego, California, United States
Edward Eng
Affiliation:
New York Structural Biology Center, New York, New York, United States
Clint Potter
Affiliation:
New York Structural Biology Center, New York, New York, United States
Bridget Carragher
Affiliation:
New York Structural Biology Center, New York, New York, United States

Abstract

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Type
Full System and Workflow Automation for Enabling Big Data and Machine Learning in Electron Microscopy
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

References

This work was performed at the Simons Electron Microscopy Center and National Resource for Automated Molecular Microscopy located at the New York Structural Biology Center (NYSBC), supported by grants from the Simons Foundation (349247), NYSTAR, and the NIH (GM103310). We thank the Columbia University Cryo-Electron Microscopy Center for access to time on their instrument housed at NYSBC.Google Scholar
Cheng, et al. Leginon: New features and applications. Protein Science, 2021, 30, 136-150.CrossRefGoogle ScholarPubMed
Cheng et al. High resolution single particle cryo-electron microscopy using beam-image shift. Journal of Structural Biology, 2018, 204, 270-275.CrossRefGoogle Scholar
Rice, et al. Routine determination of ice thickness for cryo-EM grids. Journal of Structural Biology, 2018, 204, 38-44.Google ScholarPubMed
Scheres, et al. Estimation of High-Order Aberrations and Anisotropic Magnification from Cryo-EM Datasets in RELION-3.1. IUCrJ, 2020, 7, 253267.Google Scholar