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Creating a Novel Graphene Oxide/Iron/Polylactic Acid Composite that Promotes Dental Pulp Stem Cell Proliferation and Mineralization

Published online by Cambridge University Press:  16 April 2018

Rebecca Isseroff ,
John Chen ,
Zaiff Khan ,
Anoushka Guha ,
Simon Lin ,
Juyi Li ,
Kuan-che Fang ,
Linxi Zhang ,
Marcia Simon  and
Miriam Rafailovich
Rebecca Isseroff*
Affiliation:
Stony Brook University, Stony Brook, NY11794, U.S.A. Lawrence High School, Cedarhurst, NY11516, U.S.A.
John Chen
Affiliation:
Lawrence High School, Cedarhurst, NY11516, U.S.A.
Zaiff Khan
Affiliation:
Lawrence High School, Cedarhurst, NY11516, U.S.A.
Anoushka Guha
Affiliation:
Lawrence High School, Cedarhurst, NY11516, U.S.A.
Simon Lin
Affiliation:
Stony Brook University, Stony Brook, NY11794, U.S.A.
Juyi Li
Affiliation:
Stony Brook University, Stony Brook, NY11794, U.S.A.
Kuan-che Fang
Affiliation:
Stony Brook University, Stony Brook, NY11794, U.S.A.
Linxi Zhang
Affiliation:
Stony Brook University, Stony Brook, NY11794, U.S.A.
Marcia Simon
Affiliation:
Stony Brook School of Dental Medicine, Stony Brook, NY11794, U.S.A.
Miriam Rafailovich
Affiliation:
Stony Brook University, Stony Brook, NY11794, U.S.A.
*
*(Email: rsisseroff@gmail.com)

Abstract

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Dental pulp stem cells (DPSCs) can differentiate into bone cells when provided the correct environment, potentially generating cells to repair non-union fractures. Polylactic Acid (PLA) is a biocompatible polymer for 3-D printing of scaffolds, but DPSCs do not proliferate well on PLA. With the goal of making PLA more conducive for DPSC growth, Graphene Oxide (GO); partially reduced Graphene Oxide (pRGO); GO with iron nanoparticles (FeGO) or Fe-pRGO were incorporated into PLA and spun cast as thin films onto silicon wafers for DPSC plating. DPSCs on Fe-pRGO displayed the fastest doubling time and the highest cell modulus; Fe-pRGO with exterior magnets produced high cell density. SEM demonstrated DPSC mineralization, whereas PLA-only DPSC cultures showed none. Results suggest that PLA/Fe-pRGO and PLA/pRGO enhance DPSC proliferation and possibly differentiation with the potential for use as a 3-D printed scaffold for tissue engineering.

Keywords

bonebiomaterialthin film
Type
Articles
Information
MRS Advances , Volume 3 , Issue 30: Biomaterials and Soft Materials , 2018 , pp. 1725 - 1733
Copyright
Copyright © Materials Research Society 2018 

References

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