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Fluorescence loss of commercial aqueous quantum dots during preparation for bioimaging

  • Kil Ho Lee (a1), Thomas Porter (a1) and Jessica O. Winter (a1) (a2)


Quantum dots (QDs) are increasingly employed in biologic imaging applications; however, anecdotal reports suggest difficulties in QD bioconjugation. Further, the stability of commercial QDs during bioconjugation has not been systematically evaluated. Thus, we examined fluorescence losses resulting from aggregation and declining photoluminescence quantum yield (QY) for commercial CdSe/ZnS QD products from four different vendors. QDs were most stable in the aqueous media in which they were supplied. The largest QY declines were observed during centrifugal filtration, whereas the largest declines in colloidal stability occurred in 2-(N-morpholino)ethanesulfonic acid (MES) buffer. These results enable optimization of bioconjugation protocols.


Corresponding author

Address all correspondence to Jessica O. Winter at


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These authors have contributed equally to this work.



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MRS Communications
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