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Atomic Force Microscopy Investigation of Protein Adsorption on Hydrogenated Amorphous Carbon

Published online by Cambridge University Press:  20 March 2013

Muhammad Zeeshan Mughal ,
Patrick Lemoine  and
Gennady Lubarsky
Muhammad Zeeshan Mughal
Affiliation:
Nanotechnology and Integrated BioEngineering Centre (NIBEC), University of Ulster at Jordanstown, Shore Road, Newtownabbey, BT37 0QB, United Kingdom
Patrick Lemoine
Affiliation:
Nanotechnology and Integrated BioEngineering Centre (NIBEC), University of Ulster at Jordanstown, Shore Road, Newtownabbey, BT37 0QB, United Kingdom
Gennady Lubarsky
Affiliation:
Nanotechnology and Integrated BioEngineering Centre (NIBEC), University of Ulster at Jordanstown, Shore Road, Newtownabbey, BT37 0QB, United Kingdom
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Abstract

Protein adsorption is the first phenomenon which occurs at nanoscale level when a given surface came into contact with a living fluid cell such as blood. Investigation of this adsorption at nanoscale provides useful information about kinetics and mechanism of conformation of proteins on a given surface. The present study investigates the adsorption of proteins using tapping/intermittent mode atomic force microscopy (T-AFM). The approach taken here is that hydrogenated amorphous carbon coating (a-C:H) is used as a model surface because it is amorphous, smooth, inert and hydrophobic. Two proteins namely albumin and fibrinogen in phosphate buffer (PBS) and de-ionized water are drop casted to study the adsorption kinetics. First and second resonance AFM data was used to investigate the adsorbed layer of proteins. AFM force curve and scratch experiment were used to verify the adhesion and thickness of the adsorbed layer. Combination of height, phase images along with the AFM force curve and scratch experiment shows inhomogeneous distribution of albumin protein in phosphate buffer compared to other protein solutions.

Keywords

adhesionbiomaterialscanning probe microscopy (SPM)
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1527: Symposium UU – Scanning Probe Microscopy—Frontiers in Nanotechnology , 2013 , mrsf12-1527-uu08-05
Copyright
Copyright © Materials Research Society 2013

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References

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