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Competitive adsorption of plasma proteins on polysaccharide-modified silicon surfaces

Published online by Cambridge University Press:  01 February 2011

Michela Ombelli ,
Lauren B. Costello ,
Qing Cheng Meng ,
Russell J. Composto  and
David M. Eckmann
Michela Ombelli
Affiliation:
Department of Anesthesia, University of Pennsylvania, Philadelphia, PA, 19104, U.S.A.
Lauren B. Costello
Affiliation:
Department of Materials Science and Engineering, University of Pennsylvania, Philadelphia, PA, 19104, U.S.A.
Qing Cheng Meng
Affiliation:
Department of Anesthesia, University of Pennsylvania, Philadelphia, PA, 19104, U.S.A.
Russell J. Composto
Affiliation:
Department of Materials Science and Engineering, University of Pennsylvania, Philadelphia, PA, 19104, U.S.A. Center for Bioactive Materials and Tissue Engineering, University of Pennsylvania, Philadelphia, PA, 19104, U.S.A.
David M. Eckmann
Affiliation:
Department of Anesthesia, University of Pennsylvania, Philadelphia, PA, 19104, U.S.A. The Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA, 19104, U.S.A.
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Abstract

The initial response of blood exposed to an artificial surface is the adsorption of blood proteins that triggers a number of biological reactions such as inflammation and blood coagulation. Competitive protein adsorption plays a key role in the hemocompatibility of the surface. The synthesis of nonfouling surfaces is therefore one of the major prerequisites for devices for biomedical applications. Polysaccharides are the main components of the endothelial cell glycocalyx and have the ability to reduce nonspecific protein adsorption and cell adhesion and, therefore, are generally coupled with a wide variety of surfaces to improve their biocompatibility. We have developed a procedure for covalently binding dextran and sodium hyaluronate (HA) on silicon wafers and we have been able to achieve a high level of control over the surface properties of the coatings. In the present research effort we focus on a detailed investigation of competitive bovine serum albumin (BSA) and bovine fibrinogen (Fg) adsorption on dextran- and HA-modified silicon surfaces. Polysaccharide based biomimetic layers preferentially adsorb BSA and, in general, strongly suppress protein adsorption with respect to bare silicon and APTES-activated silicon surfaces used as control.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 845: Symposium AA – Nanoscale Materials Science in Biology and Medicine , 2004 , AA8.6
Copyright
Copyright © Materials Research Society 2005

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References

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