Skip to main content Accessibility help

Development of a Novel Combined Scanning Electrochemical Microscope (SECM) and Scanning Ion-Conductance Microscope (SICM) Probe for Soft Sample Imaging

  • Andrew J. Pollard (a1), Nilofar Faruqui (a1), Michael Shaw (a1), Charles A. Clifford (a1), Yasufumi Takahashi (a2), Yuri E. Korchev (a2), Neil Ebejer (a3), Julie V. Macpherson (a3), Patrick R. Unwin (a3) and Debdulal Roy (a1)...

By incorporating a localized heating system within a scanning ion-conductance microscopy (SICM) system, we have performed stable ‘hopping-mode’ (HPICM) imaging for live cells maintained at temperatures ranging up to human body temperature. This allows the accurate study of cell volume and morphology variation versus temperature over extended periods of time. The integration of SICM with scanning electrochemical microscopy (SECM) provides the simultaneous mapping of electrochemical and topographic information for soft samples, such as live cells. This combined technique overcomes the limitations of resolution and topographical artifacts typically associated with SECM. However, previously reported SECM-SICM probe production required expensive and time-consuming focused ion beam (FIB) methods and produced pipettes that are typically hundreds of nanometers in diameter. We report a simple and rapid production method for SECM-SICM double-barrel probes with apertures down to 20 nm in diameter. The characterization of these SECM-SICM probes using scanning electron microscopy (SEM) imaging, cyclic voltammetry (CV) and Raman spectroscopy is also detailed. These SECM-SICM probes were subsequently used to study the morphology and electrochemical activity of several samples, ranging from hard metallic/insulating samples to live cells.

Hide All
1.Korchev, Y. E., Bashford, C. L., Milovanovic, M., Vodyanoy, I., and Lab, M.J., Biophys. J. 73, 653658 (1997)
2.Shevchuk, A. I., Frolenkov, G. I., Sánchez, D., James, P.S., Freedman, N., Lab, M. J., Jones, R., Klenerman, D., and Korchev, Y. E., Angew. Chem., 118, 22702274 (2006)
3.Gorelik, J., Ali, N. N., Kadir, S. H. S. A., Lab, M., Stojkovic, P., Armstrong, L., Sviderskaya, E. V., Negulyaev, Y. A., Klenerman, D., Bennett, D. C., Lako, M., Harding, S. E., Stojkovic, M., and Korchev, Y. E., Tissue Eng. C, 14, 311318 (2008)
4.Anselme, K., Biomaterials, 21, 667681 (2000)
5.Amemiya, S., Bard, A. J., Fan, F. R. F., Mirkin, M. V., Unwin, P. R., Annu. Rev. Analyt. Chem., 1, 95131 (2008)
6.Takahashi, Y., Shevchuk, A. I., Novak, P., Murakami, Y., Shiku, H., Korchev, Y. E., and Matsue, T., J. Am. Chem. Soc., 132, 1011810126 (2010)
7.Comstock, D. J., Elam, J. W., Pellin, M. J., Hersam, M. C., Anal. Chem. 82, 12701276 (2010)
8.Takahashi, Y., Shevchuk, A. I., Novak, P., Zhang, Y., Ebejer, N., Macpherson, J. V., Unwin, P. R., Pollard, A. J., Roy, D., Clifford, C. A., Shiku, H., Matsue, T., Klenerman, D., and Korchev, Y. E., Angew. Chem. Int. Ed., 50, 96389642 (2011)
9.Novak, P., Li, C., Shevchuk, A. I., Stepanyan, R., Caldwell, M., Hughes, S., Smart, T. G., Gorelik, J., Ostanin, V., Lab, M. J., Moss, G. W. J., Frolenkov, G. I., Klenerman, D., and Korchev, Y. E., Nat. Methods, 6, 279281 (2009)
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

MRS Online Proceedings Library (OPL)
  • ISSN: -
  • EISSN: 1946-4274
  • URL: /core/journals/mrs-online-proceedings-library-archive
Please enter your name
Please enter a valid email address
Who would you like to send this to? *



Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed