Hostname: page-component-8448b6f56d-c4f8m Total loading time: 0 Render date: 2024-04-25T04:54:24.648Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of Single-walled Carbon Nanotubes Entry into Mammalian Cells

Published online by Cambridge University Press:  25 October 2012

Howard H. Chen ,
Bradly Baer ,
Christopher S. Evans ,
Hannah M. Ponek  and
Michelle Chen
Howard H. Chen
Affiliation:
Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, U.S.A.
Bradly Baer
Affiliation:
Department of Chemistry, Point Loma Nazarene University, San Diego, CA 92106, U.S.A.
Christopher S. Evans
Affiliation:
Department of Chemistry, Point Loma Nazarene University, San Diego, CA 92106, U.S.A.
Hannah M. Ponek
Affiliation:
Department of Physics and Engineering, Point Loma Nazarene University, San Diego, CA 92106, U.S.A.
Michelle Chen
Affiliation:
Department of Physics and Engineering, Point Loma Nazarene University, San Diego, CA 92106, U.S.A.
Get access

Abstract

We reported a mammalian cell-imaging paradigm to study the cellular response to single-walled carbon nanotubes (SWCNTs). Chinese Hamster Ovarian (CHO) cells were exposed to SWCNTs resuspended in physiologically compatible buffer (phosphate buffered saline, PBS), at concentrations ranging from 0 to 50 μg/mL. Upon exposure, we optically imaged the cells in order to (1) visualize the accumulation SWCNTs in cells in real-time; (2) qualitatively and quantitatively assess the morphological changes associated with cellular stress in the presence of SWCNTs; and (3) serially quantify cell survival with highly sensitive bioluminescence-based imaging. Our results showed that the cell survival obtained from optical imaging agreed with that from CellTiter-Glo (CTG) luminescence viability assay. Acute compromise in the CHO cell’s survival rate was observed under high concentrations of SWCNT exposure. The cellular response as a function of SWCNT concentrations, and exposure time was further investigated.

Keywords

biologicalcellular (material form)nanostructure
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1468: Symposium VV – Nanomedicine for Molecular Imaging and Therapy , 2012 , mrss12-1468-vv05-26
Copyright
Copyright © Materials Research Society 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Liu, Z., Cai, W., He, L., Nakayama, N., Chen, K., Sun, X., Chen, X., and Dai, H., Nat. Nanotech. 2, 47–52 (2007).CrossRefGoogle Scholar
Liu, A., Chen, K., Davis, C., Sherlock, S., Cao, Q., Chen, X., and Dai, H., Cancer Res. 68, 6652 (2008).10.1158/0008-5472.CAN-08-1468CrossRefGoogle Scholar
Kam, N.W.S., O’Connell, M., Wisdom, J.A., and Dai, H., PNAS. 102, 11600–11605 (2005).CrossRefGoogle Scholar
Burke, A., ding, X., Singh, R., Kraft, R.A., Levi-Polyachenko, N., Rylander, M.N., Szot, C., Buchana, C., Whitney, J., Fisher, J., Hatcher, H.C., D’Agostino, R., Kock, N.D., Ajayan, P.M., Carroll, D.L., Akman, S., Torti, F.M., and Torti, S.V., PNAS. 106, 12897–12920 (2009).10.1073/pnas.0905195106CrossRefGoogle Scholar
de la Zerda, A., Liu, Z., Bodapati, S., Teed, R., Vaithilingam, S., Khuri-Ykub, B.T. Chen, X., Dai, H., and Gambhir, S.S., Nano Lett. 10, 2168–2172 (2010).10.1021/nl100890dCrossRefGoogle Scholar
de la Zerda, A., Zavaleta, C., Keren, S., Vaithilingam, S., Bodapati, S., Liu, Z., Levi, J., Smith, B., Ma, T.-J., Oralkan, O., Cheng, A., Chen, X., Dai, H., Khuri-Yakub, B.T., and Gambhir, S.S., Nat. Nanotech. 3, 557–562 (2008).10.1038/nnano.2008.231CrossRefGoogle Scholar
Singh, R., Pantarotto, D., Lacerda, L., Pastorin, G., Klumpp, C., Prato, M., Bianco, A., and Kostarelos, K., PNAS. 103, 3357-3362 (2006).CrossRefGoogle Scholar
Lam, C.-W., James, J.T., McCluskey, R., and Hunter, R.L., Toxicol. Sci. 77, 126-134 (2004).10.1093/toxsci/kfg243CrossRefGoogle Scholar
Schipper, M.L., Nakayama-Ratchford, N., Davis, C.R., Whi Kam, N.W., Chu, P., Liu, Z., Sun, X., Dai, H., and Gambhir, S.S., Nat. Nanotech. 3, 216–221 (2008).10.1038/nnano.2008.68CrossRefGoogle Scholar
Liu, Z., Davis, C., Cai, W., He, L., Chen, X., and Dai, H., PNAS. 105, 1410–1415 (2008).10.1073/pnas.0707654105CrossRefGoogle Scholar
Porter, A.E., Gass, M., Muller, K., Skepper, J.N., Midgley, P.A., and Welland, M., Nat. Nanotech. 2, 713–717 (2007).CrossRefGoogle Scholar
Murray, A.R., Kisin, E.R., Tkach, A.V., Yanamala, N., Mercer, R., Young, S.-H., Fadeel, B., Kagan, V.E. and Shvedova, A.A., Particle and Fibre Toxic. 9, 10 (2012).10.1186/1743-8977-9-10CrossRefGoogle Scholar