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Electropermeabilization of Mammalian Cells Visualized with Fluorescent Semiconductor Nanocrystals (Quantum Dots)

Published online by Cambridge University Press:  01 February 2011

Yinghua Sun ,
P. Thomas Vernier ,
Jingjing Wang ,
Andras Kuthi ,
Laura Marcu  and
Martin A. Gundersen
Yinghua Sun
Affiliation:
Department of Materials Science
P. Thomas Vernier
Affiliation:
Department of Electrical Engineering-Electrophysics MOSIS, Information Sciences Institute, University of Southern California, Marina del Rey, CA 90292, U.S.A
Jingjing Wang
Affiliation:
Department of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089, U.S.A
Andras Kuthi
Affiliation:
Department of Electrical Engineering-Electrophysics
Laura Marcu
Affiliation:
Department of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089, U.S.A Biophotonics Research and Technology Development, Cedars-Sinai Medical Center Los Angeles, CA 90048, U.S.A
Martin A. Gundersen
Affiliation:
Department of Materials Science Department of Electrical Engineering-Electrophysics
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Abstract

Electroporation/electropermeabilization is a non-viral technique for gene transfection and drug delivery. Here, the transfer mechanisms were studied with fluorescent nanocrystals (quantum dots, QDs) in mammalian cells. Interactions of the cell membrane and nanoscale particles were visualized after electric pulse treatment. Responses of human multiple myeloma cells to nanocrystals were tracked for periods up to 7 days. Large particles do not cross the membrane directly after pulsing, even if the membrane is permeabilized to small molecules. Large QDs were trapped on the cell membrane for hours after electroporation and were gradually either excluded or internalized by cells. QD uptake efficiency depended on both particle size and membrane transport activity. These results, consistent with an electropermeabilization model, suggest that enhancing the interactions between the cell membrane and macromolecules may improve the transfer efficiency.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 873: Symposium K – Biological and Bio-Inspired Materials and Devices , 2005 , K6.9
Copyright
Copyright © Materials Research Society 2005

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