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Migration of Marrow Stromal Cells in Response to Sustained Release of Stromal-Derived Factor-1α from Poly(lactide ethylene oxide fumarate) Hydrogels

  • Xuezhong He (a1), Junyu Ma (a2) and Esmaiel Jabbari (a3)

Stromal derived factor-1α (SDF-1α) is an important chemokine in stem cell trafficking and plays a critical role in the homing, osteogenesis as well as angiogenesis of bone marrow stromal (BMS) cells. The objective of this work was to investigate the release characteristics of SDF-1α from the degradable poly(lactide ethylene oxide fumarate) (PLEOF) hydrogels and to determine the effect of sustained release of SDF-1α on migration of BMS cells. Three PLEOF macromers with PLA content of 6, 9, and 24 by weight were synthesized by condensation polymerization. The cumulative amount of biologically-active SDF-1α released from the PLEOF hydrogels after 3 weeks was between 20-25% of the initial loading and was independent of PLA/PEG ratio in the hydrogel. The migration of BMS cells in response to the time-release SDF-1α from PLEOF hydrogels closely followed the release kinetics of SDF-1α from the hydrogels. Results demonstrate that migration of BMS cells was significantly increased by the sustained release of SDF-1α from PLEOF hydrogels.

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1Zhong, R.K., Law, P., Wong, D., Merzouk, A., Salari, H., and Ball, E.D., Small peptide analogs to stromal derived factor-1 enhance chemotactic migration of human and mouse hematopoietic cells. Exp. Hematol., 2004. 32(5): p. 470475.
2Zhu, W., Boachie-Adjei, O., Rawlins, B.A., Frenkel, B., Boskey, A.L., Ivashkiv, L.B., and Blobel, C.P., A novel regulatory role for stromal-derived factor-1 signaling in bone morphogenic protein-2 osteogenic differentiation of mesenchymal C2C12 cells. J. Biol. Chem., 2007. 282(26): p. 18676–85.
3Murphy, J.W., Cho, Y., Sachpatzidis, A., Fan, C., Hodsdon, M.E., and Lolis, E., Structural and functional basis of CXCL12 (stromal cell-derived factor-1 alpha) binding to heparin. J. Biol. Chem, 2007. 282(13): p. 10018–27.
4Huynh, D.P., Nguyen, M.K., Pi, B.S., Kim, M.S., Chae, S.Y., Kang, C.L., Bong, S.K., Kim, S.W., and Lee, D.S., Functionalized injectable hydrogels for controlled insulin delivery. Biomaterials, 2008. 29(16): p. 25272534.
5Benoit, D.S.W., Collins, S.D., and Anseth, K.S., Multifunctional hydrogels that promote osteogenic human mesenchymal stem cell differentiation through stimulation and sequestering of bone morphogenic protein 2. Adv. Funct. Mater., 2007. 17(13): p. 20852093.
6Van Tomme, S.R., Storm, G., and Hennink, W.E., In situ gelling hydrogels for pharmaceutical and biomedical applications. Int. J. Pharmaceut., 2008. 355(12): p. 118.
7Pitarresi, G., Palumbo, F.S., Giammona, G., Casadei, M.A., and Moracci, F.M., Biodegradable hydrogels obtained by photocrosslinking of dextran and polyaspartamide derivatives. Biomaterials, 2003. 24(23): p. 43014313.
8Yamamoto, M., Tabata, Y., Hong, L., Miyamoto, S., Hashimoto, N., and Ikada, Y., Bone regeneration by transforming growth factor beta 1 released from a biodegradable hydrogel. J. Contr. Rel., 2000. 64(13): p. 133142.
9He, X. and Jabbari, E., Material properties and cytocompatibility of injectable MMP degradable poly(lactide ethylene oxide fumarate) hydrogel as a carrier for marrow stromal cells. Biomacromolecules, 2007. 8(3): p. 780–92.
10Sarvestani, A.S., He, X., and Jabbari, E., Effect of osteonectin-derived peptide on the viscoelasticity of hydrogel/apatite nanocomposite scaffolds. Biopolymers, 2007. 85(4): p. 370–8.
11Sarvestani, A.S., Xu, W.J., He, X.Z., and Jabbari, E., Gelation and degradation characteristics of in situ photo-crosslinked poly(L-lactid-co-ethylene oxide-co-fumarate) hydrogels. Polymer, 2007. 48(2): p. 71137120.
12Jabbari, E. and He, X., Synthesis and characterization of bioresorbable in situ crosslinkable ultra low molecular weight poly(lactide) macromer. J. Mater. Sci. Mater. Med., 2008. 19(1): p. 311–8.
13Suggs, L.J., Krishnan, R.S., Garcia, C.A., Peter, S.J., Anderson, J.M., and Mikos, A.G., In vitro and in vivo degradation of poly(propylene fumarate-co-ethylene glycol) hydrogels. J. Biomed. Mater. Res., 1998. 42(2): p. 312320.
14He, X., Ma, J., and Jabbari, E., Effect of grafting RGD and BMP-2 protein-derived peptides to a hydrogel substrate on osteogenic differentiation of marrow stromal cells. Langmuir, 2008. 24(21): p. 12508–16.
15Ji, J.F., He, B.P., Dheen, S.T., and Tay, S.S.W., Interactions of chemokines and chemokine receptors mediate the migration of mesenchymal stem cells to the impaired site in the brain after hypoglossal nerve injury. Stem Cells, 2004. 22(3): p. 415427.
16Abbott, J.D., Huang, Y., Liu, D., Hickey, R., Krause, D.S., and Giordano, F.J., Stromal cellderived factor-1 alpha plays a critical role in stem cell recruitment to the heart after myocardial infarction but is not sufficient to induce homing in the absence of injury. Circulation, 2004. 110(21): p. 33003305.
17Ji, J.F., Dheen, S.T., Kumar, S.D., He, B.P., and Tay, S.S.W., Expressions of cytokines and chemokines in the dorsal motor nucleus of the vagus nerve after right vagotomy. Mol. Brain Res., 2005. 142(1): p. 4757.
18Mori, T., Doi, R., Koizumi, M., Toyoda, E., Ito, D., Kami, K., Masui, T., Fujimoto, K., Tamamura, H., Hiramatsu, K., Fujii, N., and Imamura, M., CXCR4 antagonist inhibits stromal cell-derived factor 1-induced migration and invasion of human pancreatic cancer. Mol. Cancer Therapeut., 2004. 3(1): p. 2937.
19Huang, X.Q., Shen, J.H., Cui, M., Shen, L.L., Luo, X.M., Ling, K., Pei, G., Jiang, H.L., and Chen, K.X., Molecular dynamics simulations on SDF-1 alpha: Binding with CXCR4 receptor. Biophys. J., 2003. 84(1): p. 171184.
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