Skip to main content

Optimized Silver Film over Nanosphere Surfaces for the Biowarfare Agent Detection Based on Surface-Enhanced Raman Spectroscopy

  • Xiaoyu Zhang (a1) and Richard P. Van Duyne (a1)

This work presents the rapid detection of Bacillus subtilis spores, harmless simulants for Bacillus anthracis, using surface-enhanced Raman spectroscopy (SERS) on silver film over nanosphere (AgFON) substrates. Calcium dipicolinate (CaDPA), a biomarker for bacillus spores, can be extracted effectively from spores with nitric acid and successfully detected by SERS. The highly tunable nature of AgFON optical properties was exploited to establish general optimization conditions. AgFON surfaces optimized for 750-nm laser excitation have been characterized by UV-vis diffuse reflectance spectroscopy. The SERS signal from extracted CaDPA was evaluated over the spore concentration range 10-15-10-12 M to determine the adsorption capacity of the AgFON surface and the limit of detection (LOD). These sensing capabilities have been successfully transitioned to an inexpensive, portable Raman spectrometer. Using the extraction method and this field-portable instrument, the anthrax infectious dose of 104 spores were detected with only a 5-second collection period on a one-month-old prefabricated AgFON substrate.

Hide All
1. McCreery R. L., Raman Spectroscopy for Chemical Analysis, (Wiley, 2000)
2. Schatz G. C. and Van Duyne R. P., Electromagnetic Mechanism of Surface-Enhanced Spectroscopy, (Wiley, 2002)
3. Haynes C. L. and Van Duyne R. P., J. Phys. Chem. B, 107, 7426, (2003).
4. Nie S. and Emory S. R., Science, 275, 1102, (1997).
5. Kneipp K., Wang Y., Kneipp H., Perelman L. T., Itzkan I., Dasari R. R. and Feld M. S., Phys. Rev. Lett., 78, 1667, (1997).
6. Sylvia J. M., Janni J. A., Klein J. D. and Spencer K. M., Anal. Chem., 72, 5834, (2000).
7. Stacy A. M. and Van Duyne R. P., Chem Phys Lett, 102, 365, (1983).
8. Kovacs G. J., Loutfy R. O., Vincett P. S., Jennings C. and Aroca R., Langmuir Langmuir, 2, 689, (1986).
9. Dick L. A., McFarland A. D., Haynes C. L. and Van Duyne R. P., J. Phys. Chem. B, 106, 853, (2002).
10. Zhang X., Yonzon C. R. and Van Duyne R. P., (Proc. SPIE-Int. Soc. Opt. Eng. 5221, San Diego, CA. 2003), pp82
11. Jensen T. R., Van Duyne R. P., Johnson S. A. and Maroni V. A., Appl. Spectrosc., 54, 371, (2000).
12. Zhang X., Young M. A., Lyandres O. and Van Duyne R. P., J. Am. Chem. Soc., 127, 4484, (2005).
13. Litorja M., Haynes C. L., Haes A. J., Jensen T. R. and Van Duyne R. P., J. Phys. Chem. B, 105, 6907, (2001).
14. Goodacre R., Shann B., Gilbert R. J., Timmins E. M., McGovern A. C., Alsberg B. K., Kell D. B. and Logan N. A., Anal. Chem., 72, 119, (2000).
15. Jarvis M. R. and Goodacre R., Anal. Chem., 76, 40, (2004).
16. Bailey G. F., Karp S. and Sacks L. E., J. Bacteriol. 89, 984, (1965).
17. Walt D. R.; Franz D. R. Anal. Chem. 72, 738A, (2000).
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

MRS Online Proceedings Library (OPL)
  • ISSN: -
  • EISSN: 1946-4274
  • URL: /core/journals/mrs-online-proceedings-library-archive
Please enter your name
Please enter a valid email address
Who would you like to send this to? *


Full text views

Total number of HTML views: 0
Total number of PDF views: 1 *
Loading metrics...

Abstract views

Total abstract views: 38 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 24th November 2017. This data will be updated every 24 hours.