Hostname: page-component-8448b6f56d-mp689 Total loading time: 0 Render date: 2024-04-18T05:13:03.949Z Has data issue: false hasContentIssue false
  • English
  • Français

Organic Hydrogel Templates for Tunable Mesoporous Silica Hybrid Materials

Published online by Cambridge University Press:  02 February 2015

Jennifer L. Kahn ,
Necla Mine Eren ,
Osvaldo Campanella ,
Sherry L. Voytik-Harbin  and
Jenna L. Rickus
Jennifer L. Kahn
Affiliation:
Department of Agricultural & Biological Engineering, Purdue University, West Lafayette, IN 47907 Physiological Sensing Facility at the Bindley Bioscience Center and Birck Nanotechnology Center, Purdue University, West Lafayette, IN 47907
Necla Mine Eren
Affiliation:
Department of Agricultural & Biological Engineering, Purdue University, West Lafayette, IN 47907
Osvaldo Campanella
Affiliation:
Department of Agricultural & Biological Engineering, Purdue University, West Lafayette, IN 47907
Sherry L. Voytik-Harbin
Affiliation:
Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907 Department of Basic Medical Sciences, Purdue University, West Lafayette, IN 47907
Jenna L. Rickus
Affiliation:
Department of Agricultural & Biological Engineering, Purdue University, West Lafayette, IN 47907 Physiological Sensing Facility at the Bindley Bioscience Center and Birck Nanotechnology Center, Purdue University, West Lafayette, IN 47907 Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907
Get access

Abstract

Porous coatings at the surface of living cells have application in human cell transplantation by controlling the transport of biomolecules to and from the cells. Sol-gel-derived mesoporous silica materials are good candidates for such coatings, owing to their biocompatibility, facile solution-based synthesis conditions, and thin film formation. Diffusion and transport across the coating correlates to long-range microstructural properties, including pore size distribution, porosity, and pore morphology. Here, we investigated collagen-fibril matrices with known biocompatibility to serve as templating systems for directed silica deposition. Type 1 collagen oligomers derived from porcine skin are extensively characterized such that we can predict and customize the final collagen-fibril matrix with respect to fibril density, interfibril branching and viscoelasticity. We show that these matrices template and direct the deposition of mesoporous silica at the level of individual collagen fibrils. We varied the fibril density, silicic acid concentration, and time of exposure to silicifying solution and characterized the resulting hybrid materials by scanning electron microscopy, energy-dispersive x-ray spectroscopy, and rheology. Microstructural properties of the collagen-fibril template are preserved in the silica surface of hybrid materials. Results for three different collagen fibril densities, corresponding to shear storage moduli of 200 Pa, 1000 Pa, and 1600 Pa, indicate that increased fibril density increases the absolute amount of templated silica when all other silica synthesis conditions are kept constant. Additionally, mechanical properties of the hybrid material are dominated by the presence of the silica coating rather than the starting collagen matrix stiffness.

Keywords

biomaterialmicrostructuresol-gel
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1721: Symposium E – Hard-Soft Interfaces in Biological and Bioinspired Materials—Bridging the Gap between Theory and Experiment , 2015 , mrsf14-1721-e05-20
Copyright
Copyright © Materials Research Society 2015 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Jaroch, D.B., Biomimetic silica encapsulation of living cells, Purdue University, 2012.Google Scholar
Kresge, C.T., Leonowicz, M.E., Roth, W.J., Vartuli, J.C., and Beck, J.S., Nature, 359, 710–712 (1992).CrossRefGoogle Scholar
Zhao, D., Jianglin, F., Huo, Q., Melosh, N., Fredrickson, G.H., Chmelka, B.F., and Stucky, G.D., Science, 279, 548–552 (1998).CrossRefGoogle Scholar
Jaroch, D.B., Lu, J., Madangopal, R., Stull, N.D., Stensberg, M., Shi, J., Kahn, J.L., Herrera-Perez, R., Zeitchek, M., Sturgis, J., Robinson, J.P., Yoder, M.C., Porterfield, D.M., Mirmira, R.G., and Rickus, J.L., AJP: Endocrinology and Metabolism, 305, E1230–E1240 (2013).Google Scholar
Niu, L.-N., Jiao, K., Ryou, H., Diogenes, A., Yiu, C.K.Y., Mazzoni, A., Chen, J.-H., Arola, D.D., Hargreaves, K.M., Pashley, D.H., and Tay, F.R., Biomacromolecules, 14, 1661–1668 (2013).CrossRefGoogle Scholar
Niu, L.-N., Jiao, K., Ryou, H., Yiu, C.K.Y., Chen, J.-H., Breschi, L., Arola, D.D., Pashley, D.H., and Tay, F.R., Angew. Chem. Int. Ed, 52, 5762–5766 (2013).CrossRefGoogle Scholar
Weiher, F., Schatz, M., Steinem, C., and Geyer, A., Biomacromolecules, 14, 683–687 (2013).CrossRefGoogle Scholar
Birchall, J.D., Chem. Soc. Rev, 24, 351 (1995).CrossRefGoogle Scholar
Kinrade, S.D., Hamilton, R.J., Schach, A.S., and Knight, C.T.G., J. Chem. Soc., Dalton Trans, 961–963 (2001).CrossRefGoogle Scholar
Kinrade, S.D., Gillson, A.-M.E., and Knight, C.T.G., J. Chem. Soc., Dalton Trans, 307–309 (2002).CrossRefGoogle Scholar
Kreger, S.T., Bell, B.J., Bailey, J., Stites, E., Kuske, J., Waisner, B., and Voytik-Harbin, S.L., Biopolymers, 93, 690–707 (2010).Google Scholar
Bailey, J.L., Critser, P.J., Whittington, C., Kuske, J.L., Yoder, M.C., and Voytik-Harbin, S.L., Biopolymers, 95, 77–93 (2011).CrossRefGoogle Scholar
Whittington, C.F., Brandner, E., Teo, K.Y., Han, B., Nauman, E., and Voytik-Harbin, S.L., Microscopy and Microanalysis, 19, 1323–1333 (2013).CrossRefGoogle Scholar