Skip to main content
    • Aa
    • Aa
  • MRS Proceedings, Volume 1498
  • January 2013, pp. 121-125

Synthesis of functionalized-thermo responsive-water soluble co-polymer for conjugation to protein for biomedical applications

  • Ali Fathi (a1), Hua Wei (a1), Wojciech Chrzanowski (a2), Anthony S. Weiss (a3) and Fariba Dehghani (a1)
  • DOI:
  • Published online: 01 January 2013

The aim of this study was to develop a thermo-responsive and bioactive polymer with suitable mechanical properties for musculoskeletal tissue engineering applications. A copolymer was synthesized that comprised of hydrophilic polyethylene glycol, thermo responsive N-isopropylacrylamide (NIPAAm), 2-hydroxyethyl methacrylate-poly(lactide) (HEMA-PLA) to enhance mechanical strength and an active N-acryloxysuccinimide (NAS) group for conjugation to proteins to enhance biological properties. A model protein such as elastin was used to assess the feasibility of conjugating this polymer to protein. The results of 1HNMR analyses confirmed that random polymerization was viable technique for synthesis of this copolymer. The co-polymers synthesized with PEG content of 3 mol% were water soluble. A hydrogel was created by dissolving the copolymer and elastin below room temperature in aqueous media, followed by rapid gelation at 37°C. The results of Fourier transform infrared analyses confirmed the conjugation of protein to copolymer due to significant reduction of ester group absorption (1735 cm−1). This data confirmed molecular interaction between protein and the temperature responsive co-polymer. Our preliminary results demonstrated that it is viable to tune different properties of this hydrogel by changing the composition of co-polymer.

Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

N. Annabi , S. M. Mithieux , A. S. Weiss , and F. Dehghani , “Cross-linked open-pore elastic hydrogels based on tropoelastin, elastin and high pressure CO2.,” Biomaterials, vol. 31, no. 7, pp. 1655–65, Mar. 2010.

N. Annabi , S. M. Mithieux , A. S. Weiss , and F. Dehghani , “The fabrication of elastin-based hydrogels using high pressure CO(2).,” Biomaterials, vol. 30, no. 1, pp. 17, Jan. 2009.

N. Annabi , S. M. Mithieux , E. a Boughton , A. J. Ruys , A. S. Weiss , and F. Dehghani , “Synthesis of highly porous crosslinked elastin hydrogels and their interaction with fibroblasts in vitro.,” Biomaterials, vol. 30, no. 27, pp. 4550–7, Sep.2009.

K. Y. Lee and D. J. Mooney , “Hydrogels for Tissue Engineering,” Chemical Reviews, vol. 101, no. 7, pp. 18691880, Jul. 2001.

C. R. Nuttelman , M. a Rice , A. E. Rydholm , C. N. Salinas , D. N. Shah , and K. S. Anseth , “Macromolecular Monomers for the Synthesis of Hydrogel Niches and Their Application in Cell Encapsulation and Tissue Engineering.,” Progress in polymer science, vol. 33, no. 2, pp. 167179, Feb. 2008.

S. R. Van Tomme , G. Storm , and W. E. Hennink , “In situ gelling hydrogels for pharmaceutical and biomedical applications.,” International journal of pharmaceutics, vol. 355, no. 1–2, pp. 118, May 2008.

R. Jin , L. S. Moreira Teixeira , P. J. Dijkstra , M. Karperien , C. a van Blitterswijk , Z. Y. Zhong , and J. Feijen , “Injectable chitosan-based hydrogels for cartilage tissue engineering.,” Biomaterials, vol. 30, no. 13, pp. 2544–51, May 2009.

D. L. Nettles , A. Chilkoti , and L. a Setton , “Applications of elastin-like polypeptides in tissue engineering.,” Advanced drug delivery reviews, vol. 62, no. 15, pp. 1479–85, Dec. 2010.

A. J. Simnick , D. W. Lim , D. Chow , and A. Chilkoti , “Biomedical and Biotechnological Applications of Elastin-Like Polypeptides,” Polymer Reviews, vol. 47, no. 1, pp. 121154, Apr. 2007.

D. W. Lim , D. L. Nettles , L. a Setton , and A. Chilkoti , “In situ cross-linking of elastin-like polypeptide block copolymers for tissue repair.,” Biomacromolecules, vol. 9, no. 1, pp. 222–30, Jan. 2008.

M. Abe , M. Takahashi , S. Tokura , H. Tamura , and A. Nagano , “Cartilage-scaffold composites produced by bioresorbable beta-chitin sponge with cultured rabbit chondrocytes.,” Tissue engineering, vol. 10, no. 3–4, pp. 585–94, 2004.

N. Iwasaki , Y. Kasahara , S. Yamane , T. Igarashi , A. Minami , and S. Nisimura , “Chitosan-Based Hyaluronic Acid Hybrid Polymer Fibers as a Scaffold Biomaterial for Cartilage Tissue Engineering,” Polymers, vol. 3, no. 1, pp. 100113, Dec. 2010.

J. Guan , Y. Hong , Z. Ma , and W. R. Wagner , “Protein-reactive, thermoresponsive copolymers with high flexibility and biodegradability.,” Biomacromolecules, vol. 9, no. 4, pp. 1283–92, Apr. 2008.

D. Neradovic , W. L. J. Hinrichs , J. J. Kettenes-van den Bosch , and W. E. Hennink , “Poly(N-isopropylacrylamide) with hydrolyzable lactic acid ester side groups: a new type of thermosensitive polymer,” Macromolecular Rapid Communications, vol. 20, no. 11, pp. 577581, Nov. 1999.

S. D. Fitzpatrick , M. a Jafar Mazumder , B. Muirhead , and H. Sheardown , “Development of injectable, resorbable drug-releasing copolymer scaffolds for minimally invasive sustained ophthalmic therapeutics.,” Acta biomaterialia, vol. 8, no. 7, pp. 2517–28, Jul. 2012.

N. Annabi , A. Fathi , S. M. Mithieux , A. S. Weiss , and F. Dehghani , “Fabrication of porous PCL/elastin composite scaffolds for tissue engineering applications,” The Journal of Supercritical Fluids, vol. 59, pp. 157167, Nov. 2011.

N. Annabi , A. Fathi , S. M. Mithieux , P. Martens , A. S. Weiss , and F. Dehghani , “The effect of elastin on chondrocyte adhesion and proliferation on poly (□-caprolactone)/elastin composites.,” Biomaterials, vol. 32, no. 6, pp. 1517–25, Feb.2011.

W. van Dijk-Wolthuls , “A new class of polymerizable dextrans with hydrolyzable groups: hydroxyethyl methacrylated dextran with and without oligolactate spacer,” Polymer, vol. 38, no. 25, pp. 6235–42, 1997.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

MRS Online Proceedings Library (OPL)
  • ISSN: -
  • EISSN: 1946-4274
  • URL: /core/journals/mrs-online-proceedings-library-archive
Please enter your name
Please enter a valid email address
Who would you like to send this to? *