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Toxicity of BSA-stabilized Silver Nanoparticles on Immune Circulating Cells

Published online by Cambridge University Press:  01 February 2011

Imani Hayman ,
Patrick Mehl ,
Veena Kapoor  and
Otto Wilson
Imani Hayman
Affiliation:
ihayman@hotmail.com, Howard University, Pharmaceutical Science, 4th & College Sts., NW, Washington, DC, 20059, United States
Patrick Mehl
Affiliation:
patrickm@vsl.cua.edu, Catholic University, Biomagnetics Group, Vitreous State Laboratory, Washington, DC, 20064, United States
Veena Kapoor
Affiliation:
veenak@helix.nih.gov, National Institutes of Health, Flow Core Facility, Experimental Transplantation and Immunology Branch, Bethesda, MD, 20892, United States
Otto Wilson
Affiliation:
wilsono@cua.edu, Catholic University, Biomedical Engineering, Washington, DC, 20064, United States
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Abstract

Silver nanoparticles have shown immense potential in many biomedical applications, specifically wound healing. These nanoparticles reduce the degree of inflammation in wounds and increase the rate of wound healing overall in a dose-dependent manner. Moreover, silver nanoparticles exhibit antibacterial and antimicrobial properties. While the mechanism of action for silver nanoparticles is not clear, current studies focus on the effect of silver nanoparticles on recipient cells and tissues. It is shown that silver nanoparticles are more toxic to these recipient cells in comparison to other metal nanoparticles. This suggests that the bactericidal properties of the silver nanoparticles are size dependent. Our present work investigates the toxicity level of silver nanoparticles on specific immune circulating cells. The approach is to report the LD50 level as a function of the ratio of the nanoparticles concentration (ppm) to the cell concentration (cell number/ml) used in the assays. This method allows a normalization of the LD50 capable to compare the toxicity of the nanoparticle on different types of cells. Next, the localization of the silver nanoparticles within the cells will be determined, and the toxic mode of action of the nanoparticles will be modulated by the modification of the synthesis method.

Keywords

Agnanoscaletissue
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1061: Symposium MM – Biomolecular and Biologically Inspired Interfaces and Assemblies , 2007 , 1061-MM09-18
Copyright
Copyright © Materials Research Society 2008

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References

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