Brunneosporella aquatica gen. et sp. nov., Aqualignicola hyalina gen. et sp. nov., Jobellisia viridifusca sp. nov. and Porosphaerellopsis bipolaris sp. nov. are described and illustrated from wood submerged in freshwater collected in Hong Kong. Both Brunneosporella and Aqualignicola have characteristic features of the Annulatascaceae and their placement within this family is discussed. Jobellisia viridifusca sp. nov. differs from all described species in having an ascomatal wall with a bright orange middle wall layer and fusiform, greenish-brown ascospores. Porosphaerellopsis bipolaris sp. nov. is unique in producing multiseptate ascospores with bipolar mucilagilous pads.