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In vivo observations of the arbuscular mycorrhizal fungus Glomus mosseae in roots by confocal laser scanning microscopy

  • HORST VIERHEILIG (a1) (a2), ANNETTE BÖCKENHOFF (a3), MICHAEL KNOBLAUCH (a3), CHRISTINE JUGE (a2), AART J. E. VAN BEL (a3), FLORIAN GRUNDLER (a1), YVES PICHÉ (a2) and URS WYSS (a1)...
    • Published online: 01 March 1999
Abstract

Confocal laser scanning microscopy was used for in vivo observations of autofluorescent fungal structures of Glomus mosseae in intact, living rye-grass roots. Clear images of hyphae and collapsed arbuscules could be obtained without staining or sectioning roots. As rye-grass roots are highly transparent, with only a few cell layers above the vascular cylinder, they provide an excellent in vivo system for microscopical confocal laser scanning studies of arbuscular mycorrhizal structures.

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Mycological Research
  • ISSN: 0953-7562
  • EISSN: 1469-8102
  • URL: /core/journals/mycological-research
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