Skip to main content
    • Aa
    • Aa
  • Get access
    Check if you have access via personal or institutional login
  • Cited by 31
  • Cited by
    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.

    Biazar, Esmaeil 2016. Application of polymeric nanofibers in medical designs, part II: Neural and cardiovascular tissues. International Journal of Polymeric Materials and Polymeric Biomaterials, Vol. 65, Issue. 18, p. 957.

    Schaub, Nicholas J. Johnson, Christopher D. Cooper, Blair and Gilbert, Ryan J. 2016. Electrospun Fibers for Spinal Cord Injury Research and Regeneration. Journal of Neurotrauma, Vol. 33, Issue. 15, p. 1405.

    Brown, Toby D. Vaquette, Cedryck Hutmacher, Dietmar W. and Dalton, Paul D. 2015. Encyclopedia of Biomedical Polymers and Polymeric Biomaterials.

    Demirbilek, Murat Sakar, Mustafa Karahaliloğlu, Zeynep Erdal, Ebru Yalçın, Eda Bozkurt, Gökhan Korkusuz, Petek Bilgiç, Elif Temuçin, Çağrı Mesut and Denkbaş, Emir Baki 2015. Aligned bacterial PHBV nanofibrous conduit for peripheral nerve regeneration. Artificial Cells, Nanomedicine, and Biotechnology, Vol. 43, Issue. 4, p. 243.

    Farrell, Kurt and Kothapalli, Chandrasekhar R. 2015. Encyclopedia of Biomedical Polymers and Polymeric Biomaterials.

    Hopkins, Amy M. DeSimone, Elise Chwalek, Karolina and Kaplan, David L. 2015. 3D in vitro modeling of the central nervous system. Progress in Neurobiology, Vol. 125, p. 1.

    Pires, Liliana R. and Pêgo, Ana P. 2015. Bridging the lesion—engineering a permissive substrate for nerve regeneration. Regenerative Biomaterials, Vol. 2, Issue. 3, p. 203.

    Pranke, Patricia Weibel, Daniel E. and Braghirolli, Daikelly I. 2015. Biodegradable Polyesters.

    Simitzi, C. Efstathopoulos, P. Kourgiantaki, A. Ranella, A. Charalampopoulos, I. Fotakis, C. Athanassakis, I. Stratakis, E. and Gravanis, A. 2015. Laser fabricated discontinuous anisotropic microconical substrates as a new model scaffold to control the directionality of neuronal network outgrowth. Biomaterials, Vol. 67, p. 115.

    Tian, L. Prabhakaran, M. P. and Ramakrishna, S. 2015. Strategies for regeneration of components of nervous system: scaffolds, cells and biomolecules. Regenerative Biomaterials, Vol. 2, Issue. 1, p. 31.

    Tian, Lingling Prabhakaran, Molamma P. Hu, Jue Chen, Menglin Besenbacher, Flemming and Ramakrishna, Seeram 2015. Coaxial electrospun poly(lactic acid)/silk fibroin nanofibers incorporated with nerve growth factor support the differentiation of neuronal stem cells. RSC Adv., Vol. 5, Issue. 62, p. 49838.

    Zuidema, Jonathan M. Provenza, Christina Caliendo, Tyler Dutz, Silvio and Gilbert, Ryan J. 2015. Magnetic NGF-Releasing PLLA/Iron Oxide Nanoparticles Direct Extending Neurites and Preferentially Guide Neurites along Aligned Electrospun Microfibers. ACS Chemical Neuroscience, Vol. 6, Issue. 11, p. 1781.

    Zuidema, Jonathan M. Desmond, Gregory P. Rivet, Christopher J. Kearns, Kathryn R. Thompson, Deanna M. and Gilbert, Ryan J. 2015. Nebulized solvent ablation of aligned PLLA fibers for the study of neurite response to anisotropic-to-isotropic fiber/film transition (AFFT) boundaries in astrocyte–neuron co-cultures. Biomaterials, Vol. 46, p. 82.

    Pires, L. R. Rocha, D. N. Ambrosio, L. and Pego, A. P. 2014. The role of the surface on microglia function: implications for central nervous system tissue engineering. Journal of The Royal Society Interface, Vol. 12, Issue. 103, p. 20141224.

    Sivolella, Stefano Brunello, Giulia Ferrarese, Nadia Della Puppa, Alessandro D'Avella, Domenico Bressan, Eriberto and Zavan, Barbara 2014. Nanostructured Guidance for Peripheral Nerve Injuries: A Review with a Perspective in the Oral and Maxillofacial Area. International Journal of Molecular Sciences, Vol. 15, Issue. 2, p. 3088.

    Brown, Toby Vaquette, Cedryck Hutmacher, Dietmar and Dalton, Paul 2013.

    Colello, Raymond J. Chow, Woon N. Bigbee, John W. Lin, Charles Dalton, Dustin Brown, Damien Jha, Balendu Shekhar Mathern, Bruce E. Lee, Kangmin D. and Simpson, David G. 2013. The incorporation of growth factor and chondroitinase ABC into an electrospun scaffold to promote axon regrowth following spinal cord injury. Journal of Tissue Engineering and Regenerative Medicine, p. n/a.

    Kim, N.J. Lee, S.J. and Atala, A. 2013. Nanomaterials in Tissue Engineering.

    Madurantakam, Parthasarathy A. Rodriguez, Isaac A. Garg, Koyal McCool, Jennifer M. Moon, Peter C. and Bowlin, Gary L. 2013. Compression of Multilayered Composite Electrospun Scaffolds: A Novel Strategy to Rapidly Enhance Mechanical Properties and Three Dimensionality of Bone Scaffolds. Advances in Materials Science and Engineering, Vol. 2013, p. 1.

    Qu, Jing Wang, Dan Wang, Huihui Dong, Yunhai Zhang, Feng Zuo, Baoqi and Zhang, Huanxiang 2013. Electrospun silk fibroin nanofibers in different diameters support neurite outgrowth and promote astrocyte migration. Journal of Biomedical Materials Research Part A, Vol. 101A, Issue. 9, p. 2667.


Evaluating neuronal and glial growth on electrospun polarized matrices: bridging the gap in percussive spinal cord injuries

  • Woon N. Chow (a1), David G. Simpson (a1), John W. Bigbee (a1) and Raymond J. Colello (a1)
  • DOI:
  • Published online: 01 August 2007

One of the many obstacles to spinal cord repair following trauma is the formation of a cyst that impedes axonal regeneration. Accordingly, we examined the potential use of electrospinning to engineer an implantable polarized matrix for axonal guidance. Polydioxanone, a resorbable material, was electrospun to fabricate matrices possessing either aligned or randomly oriented fibers. To assess the extent to which fiber alignment influences directional neuritic outgrowth, rat dorsal root ganglia (DRGs) were cultured on these matrices for 10 days. Using confocal microscopy, neurites displayed a directional growth that mimicked the fiber alignment of the underlying matrix. Because these matrices are generated from a material that degrades with time, we next determined whether a glial substrate might provide a more stable interface between the resorbable matrix and the outgrowing axons. Astrocytes seeded onto either aligned or random matrices displayed a directional growth pattern similar to that of the underlying matrix. Moreover, these glia-seeded matrices, once co-cultured with DRGs, conferred the matrix alignment to and enhanced outgrowth exuberance of the extending neurites. These experiments demonstrate the potential for electrospinning to generate an aligned matrix that influences both the directionality and growth dynamics of DRG neurites.

Corresponding author
Correspondence should be addressed to Raymond J. Colello, Department of Anatomy and Neurobiology, Virginia Commonwealth University, P.O. Box 980709, Richmond, Virginia 23298-0709, USA phone: +1 804 828 2262 fax: +1 804 827 0698 email:
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Neuron Glia Biology
  • ISSN: 1740-925X
  • EISSN: 1741-0533
  • URL: /core/journals/neuron-glia-biology
Please enter your name
Please enter a valid email address
Who would you like to send this to? *