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A question of balance: achieving appropriate nutrient levels in biofortified staple crops

  • Georgina Sanahuja (a1), Gemma Farré (a1), Judit Berman (a1), Uxue Zorrilla-López (a1), Richard M. Twyman (a2), Teresa Capell (a1), Paul Christou (a1) (a3) and Changfu Zhu (a1)...

Abstract

The biofortification of staple crops with vitamins is an attractive strategy to increase the nutritional quality of human food, particularly in areas where the population subsists on a cereal-based diet. Unlike other approaches, biofortification is sustainable and does not require anything more than a standard food-distribution infrastructure. The health-promoting effects of vitamins depend on overall intake and bioavailability, the latter influenced by food processing, absorption efficiency and the utilisation or retention of the vitamin in the body. The bioavailability of vitamins in nutritionally enriched foods should ideally be adjusted to achieve the dietary reference intake in a reasonable portion. Current vitamin biofortification programmes focus on the fat-soluble vitamins A and E, and the water-soluble vitamins C and B9 (folate), but the control of dosage and bioavailability has been largely overlooked. In the present review, we discuss the vitamin content of nutritionally enhanced foods developed by conventional breeding and genetic engineering, focusing on dosage and bioavailability. Although the biofortification of staple crops could potentially address micronutrient deficiency on a global scale, further research is required to develop effective strategies that match the bioavailability of vitamins to the requirements of the human diet.

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Corresponding author

*Corresponding author: Dr Changfu Zhu, email zhu@pvcf.udl.cat

References

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1Farré, G, Twyman, RM, Zhu, C, et al. (2011) Nutritionally enhanced crops and food security: scientific achievements versus political expediency. Curr Opin Biotechnol 22, 245251.
2Yuan, D, Bassie, L, Sabalza, M, et al. (2011) The potential impact of plant biotechnology on the Millennium Development Goals. Plant Cell Rep 30, 249265.
3Zhu, Z, Naqvi, S, Gómez-Galera, S, et al. (2007) Transgenic strategies for the nutritional enhancement of plants. Trends Plant Sci 12, 548555.
4Gómez-Galera, S, Rojas, E, Sudhakar, D, et al. (2010) Critical evaluation of strategies for mineral fortification of staple crops. Transgenic Res 19, 165180.
5Institute of Medicine (2000) Dietary Reference Intakes. Applications in Dietary Assessment. Washington, DC: National Academies Press.
6Institute of Medicine (2002) Standing Committee on the Scientific Evaluation of Dietary Reference Intakes. Dietary Reference Intakes for Energy, Carbohydrate, Fiber, Fat, Fatty Acids, Cholesterol, Protein, and Amino Acids. Washington, DC: National Academies Press.
7Institute of Medicine (2000) Food and Nutrition Board. Dietary Reference Intakes for Vitamin C, Vitamin E, Selenium, and Carotenoids. Washington, DC: National Academies Press.
8Institute of Medicine (2011) Dietary Reference Intakes (DRIs): Tolerable Upper Intake Levels, Vitamins and Elements. Food and Nutrition Board. Washington, DC: National Academies Press.
9Bai, C, Twyman, RM, Farré, G, et al. (2011) A golden era – pro-vitamin A enhancement in diverse crops. In Vitro Cell Dev Biol Plant 47, 205221.
10Shaw, GM, Schaffer, D, Velie, EM, et al. (1995) Periconceptional vitamin use, dietary folate, and the occurrence of neural tube defects. Epidemiology 6, 219226.
11Berry, RJ & Li, Z (2002) Folic acid alone prevents neural tube defects: evidence from the China study. Epidemiology 13, 114116.
12Bailey, RL, Dodd, KW, Gahche, JJ, et al. (2010) Total folate and folic acid intake from foods and dietary supplements in the United States: 2000–2006. Am J Clin Nutr 91, 231237.
13Pérez-Massot, E, Banakar, R, Gómez-Galera, S, et al. (2013) The contribution of transgenic plants to better health through improved nutrition: opportunities and constraints. Genes Nutr 8, 2941.
14Christou, P & Twyman, RM (2004) The potential of genetically enhanced plants to address food insecurity. Nutr Res Rev 17, 2342.
15Farré, G, Ramessar, K, Twyman, RM, et al. (2010) The humanitarian impact of plant biotechnology: recent breakthroughs vs bottlenecks for adoption. Curr Opin Plant Biol 13, 219225.
16Zhu, C, Sanahuja, G, Yuan, D, et al. (2013) Biofortification of plants with altered antioxidant content and composition: genetic engineering strategies. Plant Biotechnol J 11, 129141.
17Paine, JA, Shipton, CA, Chaggar, S, et al. (2005) Improving the nutritional value of Golden Rice through increased pro-vitamin A content. Nat Biotechnol 23, 482487.
18Naqvi, S, Zhu, C, Farré, G, et al. (2009) Transgenic multivitamin corn through biofortification of endosperm with three vitamins representing three distinct metabolic pathways. Proc Natl Acad Sci U S A 106, 77627767.
19Dapcich, V, Salvador Castell, G, Ribas Barba, L, et al (2004) Guía de la alimentación saludable (Guide to Healthy Eating). Madrid: Sociedad Española de Nutrición Comunitaria (Spanish Society of Community Nutrition).
20Rodriguez-Amaya, DB (1997) Carotenoids and food preparation: the retention of provitamin A carotenoids in prepared, processed, and stored foods. http://pdf.usaid.gov/pdf_docs/PNACB907.pdf (accessed accessed September 2013).
21Hart, DJ & Scott, KJ (1995) Development and evaluation of an HPLC method for the analysis of carotenoids in foods, and the measurement of the carotenoid content of vegetables and fruits commonly consumed in the UK. Food Chem 54, 101111.
22Howard, LA, Wong, A, Perry, AK, et al. (1999) β-Carotene and ascorbic acid retention in fresh and processed vegetables. J Food Sci 64, 929936.
23Lin, CH & Chen, BH (2005) Stability of carotenoids in tomato juice during processing. Eur Food Res Technol 221, 274280.
24Gayathri, GN, Platel, K, Prakash, J, et al. (2004) Influence of antioxidant spices on the retention of β-carotene in vegetables during domestic cooking processes. Food Chem 84, 3543.
25Carvalho, LMJ, Oliveira, ARG, Godoy, RLO, et al. (2012) Retention of total carotenoid and β-carotene in yellow sweet cassava (Manihot esculenta Crantz) after domestic cooking. Food Nutr Res 56, 1578815795.
26Bernhardt, S & Schlich, E (2006) Impact of different cooking methods on food quality: retention of lipophilic vitamins in fresh and frozen vegetables. J Food Eng 77, 327333.
27Mazzeo, T, N'Dri, D, Chiavaro, E, et al. (2011) Effect of two cooking procedures on phytochemical compounds, total antioxidant capacity and colour of selected frozen vegetables. Food Chem 128, 627633.
28Pomeranz, Y (1992) Biochemical, functional, and nutritive changes during storage. In Storage of Cereal Grains and Their Products, pp. 65141 [Sauer, DB, editor]. St Paul, MN: American Association of Cereal Chemists.
29Borrelli, GM, De Leonardis, AM, Platani, C, et al. (2008) Distribution along durum wheat kernel of the components involved in semolina colour. J Cereal Sci 48, 494502.
30Zielinski, H, Kozlowska, H & Lewczuk, B (2001) Bioactive compounds in the cereal grains before and after hydrothermal processing. Innov Food Sci Emerg 2, 159169.
31Finocchiaro, F, Ferrari, B, Gianinetti, A, et al. (2007) Characterization of antioxidant compounds of red and white rice and changes in total antioxidant capacity during processing. Mol Nutr Food Res 51, 10061019.
32Kanematsu, H, Ushigusa, T, Maruyama, T, et al. (1983) Comparison of tocopherol contents in crude and refined edible vegetable oils and fats by high performance liquid chromatography. J Jap Oil Chem Sot 32, 122126.
33Ortega-García, J, Gámez-Meza, N, Noriega-Rodriguez, JA, et al. (2006) Refining of high oleic safflower oil: effect on the sterols and tocopherols content. Eur Food Res Technol 223, 775779.
34Lešková, E, Kubíková, J, Kováčiková, E, et al. (2006) Vitamin losses: retention during heat treatment and continual changes expressed by mathematical models. J Food Compos Anal 19, 252276.
35Han, JS, Kozukue, N, Young, KS, et al. (2004) Distribution of ascorbic acid in potato tubers and in home-processed and commercial potato foods. J Agric Food Chem 52, 65166521.
36Azzini, E, Vitaglione, P, Intorre, F, et al. (2010) Bioavailability of strawberry antioxidants in human subjects. Br J Nutr 104, 11651173.
37Bassett, MN & Sammán, NC (2010) Folate content and retention in selected raw and processed foods. Arch Latinoam Nutr 60, 298305.
38Stea, TH, Johansson, M, Jägerstad, M, et al. (2006) Retention of folates in cooked, stored and reheated peas, broccoli and potatoes for use in modern large-scale service systems. Food Chem 101, 10951107.
39Dang, J, Arcot, J & Shrestha, A (2000) Folate retention in selected processed legumes. Food Chem 68, 295298.
40Scott, J, Rébeille, F & Fletcher, J (2000) Folic acid and folates: the feasibility for nutritional enhancement in plant foods. J Sci Food Agric 80, 795824.
41D'Ambrosio, C, Giorio, G, Marino, I, et al. (2004) Virtually complete conversion of lycopene into β-carotene in fruits of tomato plants transformed with the tomato lycopene β-cyclase (tlcy-b) cDNA. Plant Sci 166, 207214.
42Haroldsen, VM, Chi-Ham, CL, Kulkarni, S, et al. (2011) Constitutively expressed DHAR and MDHAR influence fruit, but not foliar ascorbate levels in tomato. Plant Physiol Biochem 49, 12441249.
43Díaz de la Garza, RI, Gregory, JF III & Handson, AD (2007) Folate biofortification of tomato fruit. Proc Natl Acad Sci U S A 104, 42184222.
44Seo, YS, Kim, SJ, Harn, CH, et al. (2011) Ectopic expression of apple fruit homogentisate phytyltransferase gene (MdHPT1) increases tocopherol in transgenic tomato (Solanum lycopersicum cv. Micro-Tom) leaves and fruits. Phytochemistry 72, 321329.
45Lenucci, MS, Cadinu, D, Taurino, M, et al. (2006) Antioxidant composition in cherry and high-pigment tomato cultivars. J Agric Food Chem 54, 26062613.
46Proteggente, AR, Pannala, AS, Paganga, G, et al. (2002) The antioxidant activity of regularly consumed fruit and vegetables reflects their phenolic and vitamin C composition. Free Radic Res 36, 217233.
47Bekaert, S, Storozhenko, S, Mehrshahi, P, et al. (2008) Folate biofortification in food plants. Trends Plant Sci 13, 2835.
48Berman, J, Zhu, C, Pérez-Massot, E, et al. (2013) Can the world afford to ignore biotechnology solutions that address food insecurity? Plant Mol Biol 83, 519.
49Zhu, C, Naqvi, S, Breitenbach, J, et al. (2008) Combinatorial genetic transformation generates a library of metabolic phenotypes for the carotenoid pathway in maize. Proc Natl Acad Sci U S A 105, 1823218237.
50Cho, CM, Ko, JH & Cheong, WJ (2000) Simultaneous determination of water-soluble vitamins excreted in human urine after eating an overdose of vitamin pills by a HPLC method coupled with a solid phase extraction. Talanta 51, 799806.
51Levine, M, Conry-Cantilena, C, Wang, Y, et al. (1996) Vitamin C pharmacokinetics in healthy volunteers: evidence for a recommended dietary allowance. Proc Natl Acad Sci U S A 93, 37043709.
52Smith, D, Kim, YI & Refsum, H (2008) Is folic acid good for everyone? Am J Clin Nutr 87, 517533.
53Hathcock, JN, Hattan, DG, Jenkins, MY, et al. (1990) Evaluation of vitamin A toxicity. Am J Clin Nutr 52, 183202.
54Penniston, KL & Tanumihardjo, SA (2006) The acute and chronic toxic effects of vitamin A. Am J Clin Nutr 83, 191201.
55Bramley, P, Elmadfa, I, Kafatos, A, et al. (2000) Vitamin E. J Sci Food Agric 80, 913938.
56Kappus, H & Diplock, AT (1992) Tolerance and safety of vitamin E: a toxicological position report. Free Radic Biol Med 13, 5574.
57Sizer, F, Sienkiewicz, F & Whitney, E (2008) In Nutrition: Concepts and Controversies, 11th ed., pp. 221235 [Sizer, F and Whitney, E, editors]. Belmont: Thomson Wadworth.
58EFSA Panel on Genetically Modified Organisms (GMO) (2010) Guidance on the environmental risk assessment of genetically modified plants. EFSA J 8, 1879.
59EFSA Panel on Genetically Modified Organisms (GMO) (2011) Scientific opinion on guidance for risk assessment of food and feed from genetically modified plants. EFSA J 9, 37.
60Tang, G, Qin, J, Dolnikowski, G, et al. (2009) Golden Rice is an effective source of vitamin A. Am J Clin Nutr 89, 17761783.
61Arjó, G, Capell, T, Matias-Guiu, X, et al. (2012) Mice fed on a diet enriched with genetically engineered multivitamin corn show no sub-acute toxic effects and no sub-chronic toxicity. Plant Biotechnol J 10, 10261034.
62Naqvi, S, Farré, G, Sanahuja, G, et al. (2010) When more is better: multigene engineering in plants. Trends Plant Sci 15, 4956.
63Naqvi, S, Zhu, C, Farré, G, et al. (2011) Synergistic metabolism in hybrid corn reveals bottlenecks in the carotenoid pathway and leads to the accumulation of extraordinary levels of the nutritionally important carotenoid zeaxanthin. Plant Biotechnol J 9, 384393.
64Tang, G, Hu, Y, Yin, S, et al. (2012) β-Carotene in Golden Rice is as good as β-carotene in oil at providing vitamin A to children. Am J Clin Nutr 96, 658664.
65López, AB, Van Eck, J, Conlin, BJ, et al. (2008) Effect of the cauliflower Or transgene on carotenoid accumulation and chromoplast formation in transgenic potato tubers. J Exp Bot 59, 213223.
66Li, L, Yang, Y, Xu, Q, et al. (2012) The Or gene enhances carotenoid accumulation and stability during post-harvest storage of potato tubers. Mol Plant 5, 339352.
67Hwang, FS, Stacewicz-Sapuntzakis, M & Bowen, PE (2012) Effects of heat treatment on the carotenoid and tocopherol composition of tomato. J Food Sci 77, C1109C1114.
68Dietz, JM & Gould, WA (1986) Effect of process stage and storage on retention of β-carotene in tomato juice. J Food Sci 51, 847848.
69Nagra, AS & Khan, S (1988) Vitamin A (β-carotene) losses in Pakistani cooking. J Sci Food Agric 46, 249251.
70Olunlesi, AT & Lee, CY (1979) Effect of thermal processing on the stereoizomerization of major carotenoids and vitamin A value of carrots. Food Chem 4, 311318.
71Kim, HY & Gerber, LE (1988) Influence of processing on quality of carrot juice. Korean J Food Sci Technol 20, 683690.
72Jayaraj, J, Devlin, R & Punja, Z (2008) Metabolic engineering of novel ketocarotenoid production in carrot plants. Transgenic Res 17, 489501.
73Li, Y, Wang, G, Hou, R, et al. (2011) Engineering tocopherol biosynthetic pathway in lettuce. Biol Plantarum 55, 453460.
74Yoshida, H, Hirooka, N & Kajimoto, G (1990) Microwave energy effects on quality of some seed oils. J Food Sci 55, 14121416.
75Masková, E, Rysová, J, Fiedlerová, V, et al. (1996) Stability of selected vitamins and minerals during culinary treatment of legumes. Potravinárské Védy UZPI 14, 321328.
76Jain, AK & Nessler, CL (2000) Metabolic engineering of an alternative pathway for ascorbic acid biosynthesis in plants. Mol Breeding 6, 7378.
77Bulley, S, Wright, M, Rommens, C, et al. (2012) Enhancing ascorbate in fruits and tubers through over-expression of the l-galactose pathway gene GDP-l-galactose phosphorylase. Plant Biotechnol J 10, 390397.
78Alvi, S, Khan, KM, Sheikh, MA, et al. (2003) Effect of peeling and cooking on nutrients in vegetables. Pak J Nutr 2, 189191.
79Qin, A, Shi, Q & Yu, X (2011) Ascorbic acid contents in transgenic potato plants overexpressing two dehydroascorbate reductase genes. Mol Biol Rep 38, 15571566.
80Golaszewska, B & Zalewski, S (2001) Optimisation of potato quality in culinary process. Pol J Food Nutr Sci 10, 5963.
81Nunes, ACS, Kalkmann, DC & Aragão, FJL (2009) Folate biofortification of lettuce by expression of a codon optimized chicken GTP cyclohydrolase I gene. Transgenic Res 18, 661667.
82De la Parra, C, Serna Saldivar, SO & Hai Liu, R (2007) Effect of processing on the phytochemical profiles and antioxidant activity of corn for production of masa, tortillas, and tortilla chips. J Agric Food Chem 55, 41774183.
83Li, E & Tso, P (2003) Vitamin A uptake from foods. Curr Opin Lipidol 14, 241247.
84Jeanes, YM, Hall, WL, Ellard, S, et al. (2004) The absorption of vitamin E is influenced by the amount of fat in a meal and the food matrix. Br J Nutr 92, 575579.
85Hofmann, AF (1999) Bile acids: the good, the bad, and the ugly. News Physiol Sci 14, 2429.
86Lodge, JK (2005) Vitamin E bioavailability in humans. J Plant Physiol 162, 790796.
87Yonekura, L & Nagao, A (2009) Soluble fibers inhibit carotenoid micellization in vitro and uptake by Caco-2 cells. Biosci Biotechnol Biochem 73, 196199.
88De Pee, S, West, CE, Permaesih, D, et al. (1998) Orange fruit is more effective than are dark-green, leafy vegetables in increasing serum concentrations of retinol and β-carotene in schoolchildren in Indonesia. Am J Clin Nutr 68, 10581067.
89Van het Hof, KH, Brouwer, IA, West, CE, et al. (1999) Bioavailability of lutein from vegetables is five times higher than that of β-carotene. Am J Clin Nutr 70, 261268.
90Traber, MG & Sies, H (1996) Vitamin E in humans: demand and delivery. Annu Rev Nutr 16, 321347.
91Traber, MG & Arai, H (1999) Molecular mechanisms of vitamin E transport. Annu Rev Nutr 19, 343355.
92Harrison, EH (2012) Mechanisms involved in the intestinal absorption of dietary vitamin A and provitamin A carotenoids. Biochim Biophys Acta 1821, 7077.
93Lobo, GP, Hessel, S, Eichinger, A, et al. (2010) ISX is a retinoic acid-sensitive gatekeeper that controls intestinal β,β-carotene absorption and vitamin A production. FASEB J 24, 16561666.
94Van Bennekum, A, Werder, M, Thuahnai, ST, et al. (2005) Class B scavenger receptor mediated intestinal absorption of dietary β-carotene and cholesterol. Biochemistry 44, 45174525.
95Reboul, E, Klein, A, Bietrix, F, et al. (2006) Scavenger receptor class B type I (SR-BI) is involved in vitamin E transport across the enterocyte. J Biol Chem 281, 47394745.
96Bietrix, F, Yan, D, Nauze, M, et al. (2006) Accelerated lipid absorption in mice overexpressing intestinal SR-BI. J Biol Chem 281, 72147219.
97During, A & Harrison, EH (2007) Mechanisms of provitamin A (carotenoid) and vitamin A (retinol) transport into and out of intestinal Caco-2 cells. J Lipid Res 48, 22832294.
98Borel, P, Preveraud, D & Desmarchelier, C (2013) Bioavailability of vitamin E in humans: an update. Nutr Rev 71, 319331.
99Voolstra, O, Kiefer, C, Hoehne, M, et al. (2006) The Drosophila class B scavenger receptor ninaD-I is a cell surface receptor mediating carotenoid transport for visual chromophore synthesis. Biochemistry 45, 1342913437.
100Takada, T & Suzuki, H (2010) Molecular mechanisms of membrane transport of vitamin E. Mol Nutr Food Res 54, 616622.
101Reboul, E & Borel, P (2011) Proteins involved in uptake, intracellular transport and basolateral secretion of fat-soluble vitamins and carotenoids by mammalian enterocytes. Prog Lipid Res 50, 388402.
102Oram, JF, Vaughan, AM & Stocker, R (2001) ATP-binding cassette transporter A1 mediates cellular secretion of α-tocopherol. J Biol Chem 276, 3989839902.
103Yonekura, L & Nagao, A (2007) Intestinal absorption of dietary carotenoids. Mol Nutr Food Res 51, 107115.
104Lee, SK & Kader, AA (2000) Preharvest and postharvest factors influencing vitamin C content of horticultural crops. Postharvest Biol Technol 20, 207220.
105Corti, A, Casini, AF & Pompella, A (2010) Cellular pathways for transport and efflux of ascorbate and dehydroascorbate. Arch Biochem Biophys 500, 107115.
106Malo, C & Wilson, JX (2000) Glucose modulates vitamin C transport in adult human small intestinal brush border membrane vesicles. J Nutr 130, 6369.
107Carlsson, S, Wiklund, NP, Engstrand, L, et al. (2001) Effects of pH, nitrite, and ascorbic acid on nonenzymatic nitric oxide generation and bacterial growth in urine. Nitric Oxide 5, 580586.
108Gregory, JF (2001) Case study: folate bioavailability. J Nutr 131, 1376S1382S.
109McKillop, DJ, McNulty, H, Scott, JM, et al. (2006) The rate of intestinal absorption of natural food folates is not related to the extent of folate conjugations. Am J Clin Nutr 84, 167173.
110Storozhenko, S, Navarrete, O, Ravanel, S, et al. (2007) Cytosolic hydroxymethyldihydropterine pyrophosphokinase/dihydropteroate synthase from Arabidopsis thaliana: a specific role in early development and stress response. J Biol Chem 282, 1074910761.
111Pteiffer, CM, Rogers, LM, Bailey, LB, et al. (1997) Absorption of folate from fortified cereal-grain products and of supplemental folate consumed with or without food determinate using a dual-label stable-isotope protocol. Am J Clin Nutr 66, 13881397.
112Aufreiter, S, Gregory, JF III & Pfeiffer, CM (2009) Folate is absorbed across the colon of adults: evidence from cecal infusion of 13C-labeled [6S]-5-formyltetrahydrofolic acid. Am J Clin Nutr 90, 116123.
113Gregory, JF, Williamson, J, Liao, JF, et al. (1998) Kinetic model of folate metabolism in nonpregnant women consuming [2H2] folic acid: isotopic labeling of urinary folate and the catabolite para-acetamidobenzoylglutamate indicates slow, intake-dependent, turnover of folate pools. J Nutr 128, 18961906.
114Gregory, JF (1995) The bioavailability of folate. In Folate in Health and Disease, pp. 195235 [Bailey, LB, editor]. New York: Marcel Dekker.
115Herbert, V (1987) Recommended dietary intakes (RDI) of folate in humans. Am J Clin Nutr 45, 661670.
116Farré, G, Sanahuja, G, Naqvi, S, et al. (2010) Travel advice on the road to carotenoids in plants. Plant Sci 179, 2848.
117Qaim, M (2010) Benefits of genetically modified crops for the poor: household income, nutrition, and health. Nat Biotechnol 27, 552557.
118Stein, AJ, Sachdev, HPS & Qaim, M (2006) Potential impact and cost-effectiveness of Golden Rice. Nat Biotechnol 24, 12001201.
119Institute of Medicine (1998) Dietary Reference Intakes for Thiamin, Riboflavin, Niacin, Vitamin B6, Folate, Vitamin B12, Pantothenic Acid, Biotin, and Choline. Washington, DC: National Academies Press.

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A question of balance: achieving appropriate nutrient levels in biofortified staple crops

  • Georgina Sanahuja (a1), Gemma Farré (a1), Judit Berman (a1), Uxue Zorrilla-López (a1), Richard M. Twyman (a2), Teresa Capell (a1), Paul Christou (a1) (a3) and Changfu Zhu (a1)...

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