Skip to main content
    • Aa
    • Aa
  • Get access
    Check if you have access via personal or institutional login
  • Cited by 20
  • Cited by
    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.

    Sun, Jun Wang, Suwen Li, Chen Ren, Yijiu and Wang, Jinqiang 2014. Novel expression profiles of microRNAs suggest that specific miRNAs regulate gene expression for the sexual maturation of female Schistosoma japonicum after pairing. Parasites & Vectors, Vol. 7, Issue. 1, p. 177.

    Sun, Jun Wang, Su-Wen Li, Chen Hu, Wei Ren, Yi-Jiu and Wang, Jin-Qiang 2014. Transcriptome profilings of female Schistosoma japonicum reveal significant differential expression of genes after pairing. Parasitology Research, Vol. 113, Issue. 3, p. 881.

    deWalick, Saskia Tielens, Aloysius G.M. and van Hellemond, Jaap J. 2012. Schistosoma mansoni: The egg, biosynthesis of the shell and interaction with the host. Experimental Parasitology, Vol. 132, Issue. 1, p. 7.

    Medeiros, Andrea Chiribao, María Laura Ubillos, Luis Festari, María Florencia Saldaña, Jenny Robello, Carlos Domínguez, Laura Calvete, Juan José and Osinaga, Eduardo 2008. Mucin-type O-glycosylation in Mesocestoides vogae (syn. corti). International Journal for Parasitology, Vol. 38, Issue. 3-4, p. 265.

    Fitzpatrick, Jennifer M. and Hoffmann, Karl F. 2006. Dioecious Schistosoma mansoni express divergent gene repertoires regulated by pairing. International Journal for Parasitology, Vol. 36, Issue. 10-11, p. 1081.

    Cheng, Guo-Feng Lin, Jiao-Jiao Feng, Xin-Gang Fu, Zhi-Qiang Jin, Ya-Mei Yuan, Chun-Xiu Zhou, Yuan-Cong and Cai, You-Min 2005. Proteomic analysis of differentially expressed proteins between the male and female worm ofSchistosoma japonicum after pairing. PROTEOMICS, Vol. 5, Issue. 2, p. 511.

    CHENG, Guo-Feng LIN, Jiao-Jiao SHI, Yi JIN, You-Xin FU, Zhi-Qiang JIN, Ya-Mei ZHOU, Yuan-Cong and CAI, You-Min 2005. Dose-dependent Inhibition of Gynecophoral Canal Protein Gene Expression in Vitro in the Schistosome (Schistosoma japonicum) by RNA Interference. Acta Biochimica et Biophysica Sinica, Vol. 37, Issue. 6, p. 386.

    HOFFMANN, K. F. 2005. An historical and genomic view of schistosome conjugal biology with emphasis on sex-specific gene expression. Parasitology, Vol. 128, Issue. S1, p. S11.

    Kapp, Katja Knobloch, Jürgen Schüßler, Peter Sroka, Stefan Lammers, Reiner Kunz, Werner and Grevelding, Christoph G. 2004. The Schistosoma mansoni Src kinase TK3 is expressed in the gonads and likely involved in cytoskeletal organization. Molecular and Biochemical Parasitology, Vol. 138, Issue. 2, p. 171.

    LoVerde, Philip T Niles, Edward G Osman, Ahmed and Wu, Wenjie 2004. Schistosoma mansonimale–female interactions. Canadian Journal of Zoology, Vol. 82, Issue. 2, p. 357.

    Luo, H. Y. Nie, P. Chang, M. X. Song, Y. and Yao, W. J. 2004. Characterization of development-related genes for the cestode Bothriocephalus acheilognathi. Parasitology Research, Vol. 94, Issue. 4, p. 265.

    Moné, Hélène and Boissier, Jérôme 2004. Advances in Parasitology Volume 57.

    Freire, Teresa Robello, Carlos Soulé, Silvia Ferreira, Fernando and Osinaga, Eduardo 2003. Sialyl-Tn antigen expression and O-linked GalNAc-Thr synthesis by Trypanosoma cruzi. Biochemical and Biophysical Research Communications, Vol. 312, Issue. 4, p. 1309.

    Freire, Teresa Casaravilla, Cecilia Carmona, Carlos and Osinaga, Eduardo 2003. Mucin-type O-glycosylation in Fasciola hepatica: characterisation of carcinoma-associated Tn and sialyl-Tn antigens and evaluation of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase activity. International Journal for Parasitology, Vol. 33, Issue. 1, p. 47.

    Knobloch, Jürgen Winnen, Ralf Quack, Marcus Kunz, Werner and Grevelding, Christoph G. 2002. A novel Syk-family tyrosine kinase from Schistosoma mansoni which is preferentially transcribed in reproductive organs. Gene, Vol. 294, Issue. 1-2, p. 87.

    Theodoropoulos, Georgios Hicks, Sally J Corfield, Anthony P Miller, Bevis G and Carrington, Steve D 2001. The role of mucins in host–parasite interactions: Part II – helminth parasites. Trends in Parasitology, Vol. 17, Issue. 3, p. 130.

    Kampkötter, Andreas Ridgers, Ian Johnston, David A. Rollinson, David Kunz, Werner and Grevelding, Christoph G. 1999. Schistosoma mansoni: Cloning and Characterization of the Ras Homologue. Experimental Parasitology, Vol. 91, Issue. 3, p. 280.

    Fan, Jinjiang Minchella, Dennis J. Day, Sharon R. McManus, Donald P. Tiu, Wilfred U. and Brindley, Paul J. 1998. Generation, Identification, and Evaluation of Expressed Sequence Tags from Different Developmental Stages of the Asian Blood FlukeSchistosoma japonicum. Biochemical and Biophysical Research Communications, Vol. 252, Issue. 2, p. 348.

    Finken-Eigen, Marion and Kunz, Werner 1997. Schistosoma mansoni:Gene Structure and Localization of a Homologue to Cysteine Protease ER 60. Experimental Parasitology, Vol. 86, Issue. 1, p. 1.

    Rollinson, David Kaukas, Anne Johnston, David A. Simpson, Andrew J.G. and Tanaka, Manami 1997. Some molecular insights into Schistosome evolution. International Journal for Parasitology, Vol. 27, Issue. 1, p. 11.


A female-specific cDNA sequence of Schistosoma mansoni encoding a mucin-like protein that is expressed in the epithelial cells of the reproductive duct*

  • M. Menrath (a1), A. Michel (a1) and W. Kunz (a1)
  • DOI:
  • Published online: 01 April 2009

Seven cDN A clones of Schistosoma mansoni containing the C-terminal part of the deduced sequence of a mucin-like protein have been identified. The protein contains 28% threonines, 20% serines, and has a pi of 3middot;4. On Northern blots of RNA of adult worms, the cDNA clones detect 2 transcripts of 1middot;65 and 4middot;2 kb which are expressed only in female worms. The tissue of gene expression, as revealed by in situ hybridization, is the epithelium surrounding the female reproduction duct proximal to its entrance into the ootype. Accumulation of N-glycosylation sites suggests that the protein, like other mucins, might form a protective layer, coating the lining of the duct. Regarding its acidic pi, we hypothesize a role in preventing premature egg-shell formation. This is the first female-specifically transcribed sequence, hitherto known in S. mansonithat is not expressed in the vitellaria.

Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

J. C. Armstrong (1965). Mating behaviour and development of schistosomes in the mouse. Journal of Parasitology 51, 605–16.

J. L. Bennett , J. L. Seed & M. Boff (1978). Fluorescent histochemical localization of phenol oxidase in female Schistosoma mansoni. Journal of Parasitology 64, 941–4.

L. A. Bobek , D. M. Rekosh , H. Van Keulen & P. T. Loverde (1986). Characterization of a female-specific cDNA derived from a developmentally regulated mRNA in the human blood fluke Schistosoma mansoni. Proceedings of the National Academy of Sciences, USA83, 5544–8.

J. M. Chirgwin , A. E. Przybyla , R. J. Macdonald & W. J. Rutter (1979). Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18, 5294–9.

E. R. Clough (1981). Morphology of reproductive organs and oogenesis in bisexual and unisexual transplants of mature Schistosoma mansoni females. Journal of Parasitology 67, 535–9.

J. Dietzel , J. Hirzmann , D. Preis , P. Symmons & W. Kunz (1992). Ferritins of Schistosoma mansoni: Sequence comparison and expression in female and male worms. Molecular and Biochemical Parasitology 50, 245–54.

M. Finken , A. Sobek , P. Symmons & W. Kunz (1994). Characterization of the complete protein disulfide isomerase gene of Schistosoma mansoni and identification of the tissues of its expression. Molecular and Biochemical Parasitology 64, 135–44.

K. S. Johnson , D. W. Taylor & J. S. Cordingley (1987). Possible eggshell protein gene from Schistosoma mansoni. Molecular and Biochemical Parasitology 22, 89100.

B. Köster , H. Dargatz , J. Schröder , J. Hirzmann , C. Haarmann , P. Symmons & W. Kunz (1988). Identification and localisation of the products of a putative eggshell precursor gene in the vitellarium of Schistosoma mansoni. Molecular and Biochemical Parasitology 31, 183–98.

W. Kunz , L. Gohr , C. Grevelding , P. Schüssler , G. Sommer , M. Menrath & A. Michel (1995). Schistosoma mansoni: Control of female fertility by the male. Memorias do Institute Oswaldo Cruz 90, 185–9.

W. Kunz , K. Opatz , M. Finken & P. Symmons (1987). Sequences of two genomic fragments containing an identical coding region for a putative eggshell precursor protein of Schistosoma mansoni. Nucleic Acids Research 15, 5894.

L. A. Lasky , M. S. Singer , D. Dowbenko , Y. Imai , W. J. Henzel , C. Grimley , C. Fennie , N. Gillett , S. R. Watson & S. D. Rosen (1992). An endothelial ligand for L-selectin is a novel mucin-like molecule. Cell 69, 927–38.

P. T. Loverde & L. Chen (1991). Schistosome female reproductive development. Parasitology Today 7, 303–8.

C. H. Martin , C. A. Mayeda & E. M. Meyerowitz (1988). Evolution and expression of the Sgs-3 glue gene of Drosophita. Journal of Molecular Biology 201, 273–87.

S. D. J. Pena , A. M. Macedo , V. M. M. Braga , F. D. Rumjanek & A. J. G. Simpson (1990). F10, the gene for the glycine-rich major eggshell protein of Schistosoma mansoni recognizes a family of hypervariable minisatellites in the human genome. Nucleic Acids Research 18, 7466.

M. G. Reis , J. Kuhns , R. Blanton & A. H. Davis (1989). Localization and pattern of expression of a female specific mRNA in Schistosoma mansoni. Molecular and Biochemical Parasitology 32, 113–20.

C. W. Schweinfest , K. W. Henderson , J.-R. Gu , S. D. Kottaridis , S. Besbeas , E. Panotopoulou & T. S. Papas (1990). Subtraction hybridization cDNA libraries from colon carcinoma and hepatic cancer. Genetic Analysis: Techniques and Application 7, 6470.

J. L. Seed , M. Boff & J. L. Bennett (1978). Phenol oxidase activity: induction in female schistosomes by in vitro incubation. Journal of Parasitology 64, 283–9.

G. J. Strous & J. Dekker (1992). Mucin-type glycoproteins. Critical Reviews of Biochemistry and Molecular Biology 27, 5792.

A. Swaarop , J. Xu , N. Agarwal & S. M. Weissman (1991). A simple and efficient cDNA library subtraction procedure: isolation of human retina-specific cDNA clones. Nucleic Acids Research 19, 1954.

N. W. Toribara , J. R. Gum Jr., P. J. Culhane , R. E. Lagace , J. W. Hicks , C. M. Petersen & Y. S. Kim (1991). MUC-2 human small intestinal mucin gene structure. Repeated arrays and polymorphism. Journal of Clinical Investigation 88, 1005–13.

K. E. Wells & J. S. Cordingley (1991). Schistosoma mansoni: Eggshell formation is regulated by pH and calcium. Experimental Parasitology 73, 295310.

S. West , J. Schröder & W. Kunz (1990). A multiple-staining procedure for the detection of different DNA fragments on a single blot. Analytical Biochemistry 190, 254–8.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

  • ISSN: 0031-1820
  • EISSN: 1469-8161
  • URL: /core/journals/parasitology
Please enter your name
Please enter a valid email address
Who would you like to send this to? *