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A role for antimicrobial peptides in intestinal microsporidiosis

  • G. J. LEITCH (a1) and C. CEBALLOS (a1)
  • DOI:
  • Published online: 12 December 2008

Clinical isolates from 3 microsporidia species, Encephalitozoon intestinalis and Encephalitozoon hellem, and the insect parasite Anncaliia (Brachiola, Nosema) algerae, were used in spore germination and enterocyte-like (C2Bbe1) cell infection assays to determine the effect of a panel of antimicrobial peptides. Spores were incubated with lactoferrin (Lf), lysozyme (Lz), and human beta defensin 2 (HBD2), human alpha defensin 5 (HD5), and human alpha defensin 1 (HNP1), alone and in combination with Lz, prior to germination. Of the Encephalitozoon species only E. hellem spore germination was inhibited by HNP1, while A. algerae spore germination was inhibited by Lf, HBD2, HD5 and HNP1, although HBD2 and HD5 inhibition required the presence of Lz. The effects of HBD2 and HD5 on microsporidia enterocyte infection paralleled their effects on spore germination. Lysozyme alone only inhibited infection with A. algerae, while Lf inhibited infection by E. intestinalis and A. algerae. HNP1 significantly reduced enterocyte infection by all 3 parasite species and a combination of Lf, Lz and HNP1 caused a further reduced infection with A. algerae. These data suggest that intestinal antimicrobial peptides contribute to the defence of the intestine against infection by luminal microsporidia spores and may partially determine which parasite species infects the intestine.

Corresponding author
*Corresponding author: The Whitney Laboratory for Marine Bioscience, University of Florida, 9505 Ocean Shore Blvd., St Augustine, FL 32080, USA. Tel: +904 461 4000. Fax: +904 461 4052. E-mail:
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E. Bard , S. Laibe , D. Bettinger , D. Riethmuller , S. Buchle , E. Seilles and D. Meillet (2003). New sensitive method for the measurement of lysozyme and lactoferrin for assessment of innate mucosal immunity. Part II: time-resolved immunofluorometric assay in serum and mucosal secretions. Clinical Chemistry and Laboratory Medicine 41, 127133.

K. A. Brogden (2005). Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria? Nature Reviews Microbiology 3, 238250.

A. Cali , L. M. Weiss and P. M. Takvorian (2002). Brachiola algerae spore membrane systems, their activity during extrusion, and a new structural entity, the multilayered interlaced network, associated with the polar tube and the sporoplasm. Journal of Eukaryotic Microbiology 49, 164174.

X. Chen , F. Niyonsaba , H. Ushio , D. Okuda , I. Nagaoka , S. Ikeda , K. Okumura and H. Ogawa (2005). Synergistic effect of antibacterial agents human beta-defensins, cathelicidin LL-37 and lysozyme against Staphylococcus aureus and Escherichia coli. Journal of Dermatological Science 40, 123132.

C. M. Coyle , L. M. Weiss , V. Rhodes , A. Cali , P. M. Takvorian , F. Brown , G. S. Visvesvara , L. Xiao , J. Naktin , E. Young , M. Gareca , G. Colasante and M. Wittner (2004). Fatal myositis due to the microsporidian Brachiola algerae, a mosquito pathogen. New England Journal of Medicine 351, 4247.

E. Frixione , L. Ruiz , J. Cerbon and A. H. Undeen (1997). Germination of Nosema algerae (Microspora) spores: conditional inhibition by D2O, ethanol and Hg2+ suggests dependence of water influx upon membrane hydration and specific transmembrane pathways. Journal of Eukaryotic Microbiology 44, 109116.

S. Joly , C. Maze , P. B. McCray Jr. and J. M. Guthmiller (2004). Human β-defensins 2 and 3 demonstrate strain-selective activity against oral microorganisms. Journal of Clinical Microbiology 42, 10241029.

E. M. Keohane and L. M. Weiss (1999). The structure, function, and composition of the microsporidian polar tube. In The Microsporidia and Microsporidiosis (ed. M. Whittner and L. M. Weiss ), pp. 196224. American Society for Microbiology Press, Washington, D.C., USA.

A. Muller , R. Bialek , A. Kamper , G. Fatkenheuer , B. Salzberger and C. Franzen (2001). Detection of microsporidia in travelers with diarrhea. Journal of Clinical Microbiology 39, 6301632.

I. Nagaoka , S. Hirota , S. Yamogida , A. Ohwada , and M. Hirata (2000). Synergistic actions of antibacterial neutrophil defensins and cathelicidins. Inflammation Research 49, 7379.

G. S. Visvesvara , H. Moura , G. J. Leitch and D. A. Schwartz (1999). Culture and propagation of microsporidia. In The Microsporidia and Microsporidiosis (ed. M. Whittner and L. M. Weiss ), pp. 363392. American Society for Microbiology Press, Washington, D.C., USA.

S. Vylkova , N. Nayyar , W. Li and M. Edgerton (2007). Human beta defensins kill Candida albicans in an energy-dependent and salt–sensitive manner without causing membrane disruption. Antimicrobial Agents and Chemotherapy 51, 154161.

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