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Parasitology Parasitology

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MALDI-TOF MS protein profiling for the rapid identification of Chagas disease triatomine vectors and application to the triatomine fauna of French Guiana

Published online by Cambridge University Press:  03 August 2017

MAUREEN LAROCHE ,
JEAN-MICHEL BÉRENGER ,
GLADYS GAZELLE ,
DENIS BLANCHET ,
DIDIER RAOULT  and
PHILIPPE PAROLA
MAUREEN LAROCHE
Affiliation:
Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE), IHU Méditerranée Infection, Aix-Marseille University, UM63, CNRS 7278, IRD 198 (Dakar, Sénégal), Inserm 1095, 19-21 bd Jean Moulin, 13385 Marseille cedex 5, France
JEAN-MICHEL BÉRENGER
Affiliation:
Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE), IHU Méditerranée Infection, Aix-Marseille University, UM63, CNRS 7278, IRD 198 (Dakar, Sénégal), Inserm 1095, 19-21 bd Jean Moulin, 13385 Marseille cedex 5, France
GLADYS GAZELLE
Affiliation:
Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE), IHU Méditerranée Infection, Aix-Marseille University, UM63, CNRS 7278, IRD 198 (Dakar, Sénégal), Inserm 1095, 19-21 bd Jean Moulin, 13385 Marseille cedex 5, France
DENIS BLANCHET
Affiliation:
Laboratoire Hospitalier Universitaire de Parasitologie et Mycologie, Centre Hospitalier A. Rosemon, Cayenne, France
DIDIER RAOULT
Affiliation:
Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE), IHU Méditerranée Infection, Aix-Marseille University, UM63, CNRS 7278, IRD 198 (Dakar, Sénégal), Inserm 1095, 19-21 bd Jean Moulin, 13385 Marseille cedex 5, France
PHILIPPE PAROLA*
Affiliation:
Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE), IHU Méditerranée Infection, Aix-Marseille University, UM63, CNRS 7278, IRD 198 (Dakar, Sénégal), Inserm 1095, 19-21 bd Jean Moulin, 13385 Marseille cedex 5, France
*
*Corresponding author: Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE), Faculté de Médecine, 19-21 bd Jean Moulin, 13385 Marseille cedex 5, France. E-mail: philippe.parola@univ-amu.fr
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Summary

Triatomines are haematophagous insects involved in the transmission of Trypanosoma cruzi, the aetiological agent of Chagas disease. The vector competence of these arthropods can be highly variable, depending on the species. A precise identification is therefore crucial for the epidemiological surveillance of T. cruzi and the determination of at-risk human populations. To circumvent the difficulties of morphological identification and the lack of comprehensiveness of the GenBank database, we hereby propose an alternative method for triatomine identification. The femurs of the median legs of triatomines from eight different species from French Guiana were subjected to matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) analysis. Method evaluation was performed on fresh specimens and was applied to dry specimens collected between 1991 and 2003. Femur-derived protein extracts provided reproducible spectra within the same species along with significant interspecies heterogeneity. Validation of the study by blind test analysis provided 100% correct identification of the specimens in terms of the species, sex and developmental stage. MALDI-TOF mass spectrometry appears to be a powerful tool for triatomine identification, which is a major step forward in the fight against Chagas disease.

Keywords

TriatominaeMALDI-TOFFrench Guianaarthropod identification
Type
Special Issue Article
Information
Parasitology , Volume 145 , Special Issue 5: Advances and challenges in barcoding of microbes, parasites, and their vectors and reservoirs , April 2018 , pp. 665 - 675
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Copyright
Copyright © Cambridge University Press 2017 

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References

Berenger, J.-M., Pluot-Sigwalt, D., Pagés, F., Blanchet, D. and Aznar, C. (2009). The Triatominae species of French Guiana (Heteroptera: Reduviidae). Memórias do Instituto Oswaldo Cruz 104, 1111–1116.CrossRefGoogle Scholar
Dieme, C., Yssouf, A., Vega-Rúa, A. and Berenger, J. M. (2014 a). Accurate identification of Culicidae at aquatic developmental stages by MALDI-TOF MS profiling. Parasites & Vectors 7, 544.CrossRefGoogle ScholarPubMed
Dieme, C., Yssouf, A., Vega-Rúa, A., Berenger, J.-M., Failloux, A.-B., Raoult, D., Parola, P. and Almeras, L. (2014 b). Accurate identification of Culicidae at aquatic developmental stages by MALDI-TOF MS profiling. Parasites & Vectors 7, 544.CrossRefGoogle ScholarPubMed
Dujardin, J.-P., Lam, T. X., Khoa, P. T. and Schofield, C. J. (2015). The rising importance of Triatoma rubrofasciata. Memórias do Instituto Oswaldo Cruz 110, 319–323.CrossRefGoogle ScholarPubMed
Elliot, S. L., Adler, F. R. and Sabelis, M. W. (2003). How virulent should a parasite be to its vector? Ecology 84, 2568–2574.CrossRefGoogle Scholar
Elliot, S. L., de Rodrigues, J. O., Lorenzo, M. G., Martins-Filho, O. A. and Guarneri, A. A. (2015). Trypanosoma cruzi, etiological agent of Chagas disease, is virulent to its triatomine vector Rhodnius prolixus in a temperature-dependent manner. PLoS Neglected Tropical Diseases 9, e0003646.CrossRefGoogle Scholar
Fellet, M. R., Lorenzo, M. G., Elliot, S. L., Carrasco, D. and Guarneri, A. A. (2014). Effects of infection by Trypanosoma cruzi and Trypanosoma rangeli on the reproductive performance of the vector Rhodnius prolixus. PLoS ONE 9, e105255.CrossRefGoogle ScholarPubMed
Folmer, O., Black, M., Hoeh, W., Lutz, R. and Vrijenhoek, R. (1994). DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3, 294–299.Google ScholarPubMed
Fotso Fotso, A., Mediannikov, O., Diatta, G., Almeras, L., Flaudrops, C., Parola, P. and Drancourt, M. (2014). MALDI-TOF mass spectrometry detection of pathogens in vectors: the Borrelia crocidurae/Ornithodoros sonrai paradigm. PLoS Neglected Tropical Diseases 8, e2984.CrossRefGoogle ScholarPubMed
Galvão, C. and Justi, S. A. (2015). An overview on the ecology of Triatominae (Hemiptera: Reduviidae). Acta Tropica 151, 116–125.CrossRefGoogle Scholar
Hoppenheit, A., Murugaiyan, J., Bauer, B., Clausen, P.-H. and Roesler, U. (2014). Analysis of Glossina palpalis gambiensis and Glossina tachinoides from two distant locations in Burkina Faso using MALDI TOF MS. Parasitology Research 113, 723–726.CrossRefGoogle ScholarPubMed
Kaufmann, C., Ziegler, D., Schaffner, F., Carpenter, S., Pflüger, V. and Mathis, A. (2011). Evaluation of matrix-assisted laser desorption/ionization time of flight mass spectrometry for characterization of Culicoides nubeculosus biting midges. Medical and Veterinary Entomology 25, 32–38.CrossRefGoogle ScholarPubMed
Kaufmann, C., Schaffner, F., Ziegler, D., Pflüger, V. and Mathis, A. (2012). Identification of field-caught Culicoides biting midges using matrix-assisted laser desorption/ionization time of flight mass spectrometry. Parasitology 139, 248–258.CrossRefGoogle ScholarPubMed
Kumsa, B., Laroche, M., Almeras, L., Mediannikov, O., Raoult, D. and Parola, P. (2016). Morphological, molecular and MALDI-TOF mass spectrometry identification of ixodid tick species collected in Oromia, Ethiopia. Parasitology Research 115, 4199–4210.CrossRefGoogle ScholarPubMed
Lafri, I., Almeras, L., Bitam, I., Caputo, A., Yssouf, A., Forestier, C.-L., Izri, A., Raoult, D. and Parola, P. (2016). Identification of Algerian field-caught Phlebotomine sand fly vectors by MALDI-TOF MS. PLoS Neglected Tropical Diseases 10, e0004351.CrossRefGoogle ScholarPubMed
Laroche, M., Almeras, L., Pecchi, E., Bechah, Y., Raoult, D., Viola, A. and Parola, P. (2017). MALDI-TOF MS as an innovative tool for detection of Plasmodium parasites in Anopheles mosquitoes. Malaria Journal 16, 5.CrossRefGoogle ScholarPubMed
Lazzari, C. R., Pereira, M. H., Lorenzo, M. G., Lazzari, C. R., Pereira, M. H. and Lorenzo, M. G. (2013). Behavioural biology of Chagas disease vectors. Memórias do Instituto Oswaldo Cruz 108, 34–47.CrossRefGoogle ScholarPubMed
Lo, C. I., Fall, B., Sambe-Ba, B., Diawara, S., Gueye, M. W., Mediannikov, O., Sokhna, C., Faye, N., Diemé, Y., Wade, B., Raoult, D. and Fenollar, F. (2015). MALDI-TOF mass spectrometry: a powerful tool for clinical microbiology at Hôpital Principal de Dakar, Senegal (West Africa). PLoS ONE 10, e0145889.CrossRefGoogle Scholar
Longo, D. L. and Bern, C. (2015). Chagas’ disease. New England Journal of Medicine 373, 456–466.Google Scholar
Lv, J., Wu, S., Zhang, Y., Chen, Y., Feng, C., Yuan, X., Jia, G., Deng, J., Wang, C., Wang, Q., Mei, L. and Lin, X. (2014). Assessment of four DNA fragments (COI, 16S rDNA, ITS2, 12S rDNA) for species identification of the Ixodida (Acari: Ixodida). Parasites & Vectors 7, 93.CrossRefGoogle Scholar
Marliére, N. P., Latorre-Estivalis, J. M., Lorenzo, M. G., Carrasco, D., Alves-Silva, J., de Rodrigues, J. O. de Ferreira, L. L., de Lara, L. M., Lowenberger, C. and Guarneri, A. A. (2015). Trypanosomes modify the behavior of their insect hosts: effects on locomotion and on the expression of a related gene. PLoS Neglected Tropical Diseases 9, e0003973.CrossRefGoogle ScholarPubMed
Monteiro, F. A., Escalante, A. A. and Beard, C. B. (2001). Molecular tools and triatomine systematics: a public health perspective. Trends in Parasitology 17, 344–347.CrossRefGoogle ScholarPubMed
Pavan, M. G. and Monteiro, F. A. (2007). A multiplex PCR assay that separates Rhodnius prolixus from members of the Rhodnius robustus cryptic species complex (Hemiptera: Reduviidae). Tropical Medicine & International Health 12, 751–758.CrossRefGoogle Scholar
Pita, S., Lorite, P., Nattero, J., Galvão, C., Alevi, K. C. C., Teves, S. C., Azeredo-Oliveira, M. T. V. and Panzera, F. (2016). New arrangements on several species subcomplexes of Triatoma genus based on the chromosomal position of ribosomal genes (Hemiptera – Triatominae). Infection, Genetics and Evolution 43, 225–231.CrossRefGoogle Scholar
Sambou, M., Aubadie-Ladrix, M., Fenollar, F., Fall, B., Bassene, H., Almeras, L., Sambe-Ba, B., Perrot, N., Chatellier, S., Faye, N., Parola, P., Wade, B., Raoult, D. and Mediannikov, O. (2015). Comparison of matrix-assisted laser desorption ionization-time of flight mass spectrometry and molecular biology techniques for identification of Culicoides (Diptera: Ceratopogonidae) biting midges in Senegal. Journal of Clinical Microbiology 53, 410–418.CrossRefGoogle ScholarPubMed
Schuh, R. T. and Slater, J. A. (1995). True Bugs of the World (Hemiptera:Heteroptera). Cornell University Press, Ithaca, NY, USA.Google Scholar
Singhal, N., Kumar, M., Kanaujia, P. K. and Virdi, J. S. (2015). MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis. Frontiers in Microbiology 6, 791.CrossRefGoogle ScholarPubMed
Weirauch, C. and Munro, J. B. (2009). Molecular phylogeny of the assassin bugs (Hemiptera: Reduviidae), based on mitochondrial and nuclear ribosomal genes. Molecular Phylogenetics and Evolution 53, 287–299.CrossRefGoogle Scholar
Yssouf, A., Flaudrops, C., Drali, R., Kernif, T., Socolovschi, C., Berenger, J.-M., Raoult, D. and Parola, P. (2013 a). Matrix-assisted laser desorption ionization-time of flight mass spectrometry for rapid identification of tick vectors. Journal of Clinical Microbiology 51, 522–528.CrossRefGoogle ScholarPubMed
Yssouf, A., Socolovschi, C., Flaudrops, C., Ndiath, M. O., Sougoufara, S., Dehecq, J.-S., Lacour, G., Berenger, J.-M., Sokhna, C. S., Raoult, D. and Parola, P. (2013 b). Matrix-assisted laser desorption ionization – time of flight mass spectrometry: an emerging tool for the rapid identification of mosquito vectors. PLoS ONE 8, e72380.CrossRefGoogle ScholarPubMed
Yssouf, A., Parola, P., Lindström, A., Lilja, T., L'Ambert, G., Bondesson, U., Berenger, J.-M., Raoult, D. and Almeras, L. (2014 a). Identification of European mosquito species by MALDI-TOF MS. Parasitology Research 113, 2375–2378.CrossRefGoogle ScholarPubMed
Yssouf, A., Socolovschi, C., Leulmi, H., Kernif, T., Bitam, I., Audoly, G., Almeras, L., Raoult, D. and Parola, P. (2014 b). Identification of flea species using MALDI-TOF/MS. Comparative Immunology, Microbiology and Infectious Diseases 37, 153–157.CrossRefGoogle ScholarPubMed
Yssouf, A., Almeras, L., Berenger, J.-M., Laroche, M., Raoult, D. and Parola, P. (2015 a). Identification of tick species and disseminate pathogen using hemolymph by MALDI-TOF MS. Ticks and Tick-Borne Diseases 6, 579–586.CrossRefGoogle ScholarPubMed
Yssouf, A., Almeras, L., Terras, J., Socolovschi, C., Raoult, D. and Parola, P. (2015 b). Detection of Rickettsia spp in ticks by MALDI-TOF MS. PLoS Neglected Tropical Diseases 9, e0003473.CrossRefGoogle ScholarPubMed
Yssouf, A., Almeras, L., Raoult, D. and Parola, P. (2016). Emerging tools for identification of arthropod vectors. Future Microbiology 11, 549–566.CrossRefGoogle ScholarPubMed
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MALDI-TOF MS protein profiling for the rapid identification of Chagas disease triatomine vectors and application to the triatomine fauna of French Guiana
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