Skip to main content
    • Aa
    • Aa

Antibodies to the glutamate dehydrogenase of Plasmodium falciparum

  • I. T. Ling (a1), S. Cooksley (a2), P. A. Bates (a1), E. Hempelmann (a3) and R. J. M. Wilson (a1)...

Polyclonal antisera raised against Plasmodium knowlesi reacted with (1) NADP-specific glutamate dehydrogenase (GLDH) of P. knowlesi, (2) GLDH of P. falciparum and (3) GLDH of Proteus spp. The antisera did not react with NAD(P) GLDH from bovine liver. Polyclonal antisera raised against the GLDH of Proteus spp. cross-reacted with GLDH from P. falciparum. Monoclonal antibodies (McAbs) obtained from mice immunized with Proteus GLDH were either specific for the bacterial enzyme or cross-reacted with P. falciparum GLDH. The selected McAbs did not react with GLDI-1 from P. knowlesi, P. chabaudi or P. berghei. The GLDH of P. falciparum was shown to be a cytosolic protein (by FAT) with a subunit molecular weight of approximately 49000 Da (by immunoprecipitation) having a pre dominantly hexameric form (by sucrose density gradient). Implications of the conserved sequences of GLDHs and other enzymes are discussed.

Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

M. Brett , G. K. Chambers , A. A. Holder , J. R. S. Fincham & J. C. Wooton (1976). Mutational amino acid replacements in Neurospora crassa NADP-specific glutamate dehydrogenase. Journal of Molecular Biology 106, 122.

A. R. Dluzewski , I. T. Ling , K. Rangachari , P. A. Bates & R. J. M. Wilson (1984). A simple method for isolating viable mature parasites of Plasmodium falciparum from cultures. Transactions of the Royal Society of Tropical Medicine and Hygiene 78, 622–4.

G. Galfre , J. C. Howe , C. Milstein , G. W. Butcher & J. C. Howard (1977). Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature, London 266, 550–2.

E. Hempelmann , I. Ling & R. J. M. Wilson (1981). S-antigens and isozymes in strains of Plasmodium falciparum. Transactions of the Royal Society of Tropical Medicine and Hygiene 75, 855–8.

V. Horejsi & I. Hilgent (1983). Nitrocellulose membrane as an antigen or antibody carrier for screening hybridoma cultures. Journal of Immunological Methods 62, 325–9.

D. J. Kemp , I. M. Corcoran , R. L. Coppel , H. D. Stahl , A. E. Bianco , C. V. Brown & R. F. Anders (1985). Size variation in chromosomes from independent cultured isolates of Plasmodium falciparum. Nature, London 315, 347–9.

U. K. Laemmli (1970). Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature, London 227, 680–5.

A. Martinez-Ramon & J. Renau-Piqueras (1984). Monoclonal antibody to glutamate dehydro genase. Isolation and characterisation. Cell Biology International Reports 8, 665–74.

I. W. Mattaj , M. J. McPherson & J. C. Wootton (1982). Localisation of a strongly conserved section of coding sequence in glutamate dehydrogenase genes. Federation of European Biochemical Societies, Letters 147, 21–5.

T. F. McCutchan , J. B. Dame , L. H. Miller & J. Barnwell (1984). Evolutionary relatedness of Plasmodium species as determined by the structure of DNA. Science 225, 808–11.

D. B. Roberts & J. A. Pateman (1964). Immunological studies of amination deficient strains of Neurospora crassa. Journal of General Microbiology 34, 295305.

E. L. Smith , B. M. Austen , K. M. Blumsntithal & J. F. Nyc (1975). Glutamate dehydrogenases. In The Enzymes, vol. 11 (ed. P. D. Boyer ), pp. 293367. New York: Academic Press.

R. D. Walter , J.-P. Nordmeyer & E. Königk (1974). NADP-specific glutamate dehydrogenase from Plasmodium chabaudi. Hoppe-Seyler's Zeitschrift für Physiologische Chemie 355, 495500.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

  • ISSN: 0031-1820
  • EISSN: 1469-8161
  • URL: /core/journals/parasitology
Please enter your name
Please enter a valid email address
Who would you like to send this to? *


Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 3 *
Loading metrics...

Abstract views

Total abstract views: 141 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 23rd May 2017. This data will be updated every 24 hours.