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Assessment of monoclonal antibodies to Echinococcus granulosus Antigen 5 and Antigen B for detection of human hydatid circulating antigens

  • D. Liu (a1), M. D. Rickard (a2) and M. W. Lightowlers (a1)
  • DOI: http://dx.doi.org/10.1017/S0031182000074849
  • Published online: 01 April 2009
Abstract
SUMMARY

Four monoclonal antibodies (MAb) to Echinococcus granulosus Antigen 5 (Ag5) and Antigen B (AgB) were assessed in an enzyme-linked immunosorbent assay (ELISA) for detection of circulating antigens (CAg) in sera of human patients with E. granulosus infection. Around 5·5–8% of 200 sera from 42 surgically proven hydatid patients contained detectable CAg by individual MAb. The combined detection rate for CAg, using four MAb, was 19% (38/200). Although hydatid CAg was detected by MAb in at least one serum sample from 21 of 42 patients, some patients remained negative in the assay regardless of the time when serum samples were taken (pre- or post-operatively), or of the continuing presence of hydatid cysts, their location or fertility. In addition, it was observed that the binding capacity of MAb for sheep hydatid cyst fluid antigen (SHCF) was somewhat reduced in the presence of normal human serum. The CAg detection assay would only be useful for assessment of hydatid infection status in patients with detectable CAg in serum samples.

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Corresponding author
*D. Liu, Department of Food and Agriculture, Pastoral and Veterinary Institute, Ballarat Road, Hamilton, Victoria 3300, Australia
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M. W. Lightowlers , D. Liu , A. Haralambous & M. D. Rickard (1989). Subunit composition and specificity of the major cyst fluid antigens of Echinococcus granulosus. Molecular and Biochemical Parasitology 37, 171–82.

D. Liu , M. D. Rickard & M. W. Lightowlers (1992 b). Further characterisation of monoclonal antibodies to Echinococcus granulosus Antigen 5 and Antigen B. International Journal for Parasitology 22, 391–4.

D. Richard-Lenoble , M. D. Smith , M. Loisy & P. J. Verroust (1978). Human hydatidosis: evaluation of three serodiagnostic methods, the principal subclass of specific immunoglobulin and the detection of circulating immune complexes. Annals of Tropical Medicine and Parasitology 72, 553–60.

M. D. Rickard , R. D. Honey , J. L. Brumley & G. F. Mitchell (1984). Serological diagnosis and post-operative surveillance of human hydatid disease. II. The enzyme-linked immunosorbent assay (ELISA) using various antigens. Pathology 16, 211–15.

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