Goldman-Pinkovich, Adele Balno, Caitlin Strasser, Rona Zeituni-Molad, Michal Bendelak, Keren Rentsch, Doris Ephros, Moshe Wiese, Martin Jardim, Armando Myler, Peter J. Zilberstein, Dan and Müller, Ingrid 2016. An Arginine Deprivation Response Pathway Is Induced in Leishmania during Macrophage Invasion. PLOS Pathogens, Vol. 12, p. e1005494.
Bittencourt, Clicia Ramos de Oliveira Farias, Emanuel Airton Bezerra, Karla Costa Véras, Leiz Maria Costa Silva, Vladimir Costa Costa, Carlos Henrique Nery Bemquerer, Marcelo P. Silva, Luciano Paulino Souza de Almeida Leite, José Roberto de and Eiras, Carla 2016. Immobilization of cationic antimicrobial peptides and natural cashew gum in nanosheet systems for the investigation of anti-leishmanial activity. Materials Science and Engineering: C, Vol. 59, p. 549.
De Pablos, LM Ferreira, TR and Walrad, PB 2016. Developmental differentiation in Leishmania lifecycle progression: post-transcriptional control conducts the orchestra. Current Opinion in Microbiology, Vol. 34, p. 82.
Singh, Kuljit Singh, Krishn Pratap Equbal, Asif Suman, Shashi S. Zaidi, Amir Garg, Gaurav Pandey, Krishna Das, Pradeep and Ali, Vahab 2016. Interaction between cysteine synthase and serine O-acetyltransferase proteins and their stage specific expression in Leishmania donovani. Biochimie, Vol. 131, p. 29.
Mittra, Bidyottam Laranjeira-Silva, Maria Fernanda Perrone Bezerra de Menezes, Juliana Jensen, Jennifer Michailowsky, Vladimir Andrews, Norma W. and Horn, David 2016. A Trypanosomatid Iron Transporter that Regulates Mitochondrial Function Is Required for Leishmania amazonensis Virulence. PLOS Pathogens, Vol. 12, p. e1005340.
Argueta-Donohué, Jesús Wilkins-Rodríguez, Arturo A. Aguirre-García, Magdalena and Gutiérrez-Kobeh, Laila 2016. Differential phagocytosis ofLeishmania mexicanapromastigotes and amastigotes by monocyte-derived dendritic cells. Microbiology and Immunology, Vol. 60, p. 369.
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Tsigankov, Polina Gherardini, Pier Federico Helmer-Citterich, Manuela Späth, Gerald F. Myler, Peter J. and Zilberstein, Dan 2014. Regulation Dynamics ofLeishmaniaDifferentiation: Deconvoluting Signals and Identifying Phosphorylation Trends. Molecular & Cellular Proteomics, Vol. 13, p. 1787.
Saunders, Eleanor C. Ng, William W. Kloehn, Joachim Chambers, Jennifer M. Ng, Milica McConville, Malcolm J. and Wilson, Mary E. 2014. Induction of a Stringent Metabolic Response in Intracellular Stages of Leishmania mexicana Leads to Increased Dependence on Mitochondrial Metabolism. PLoS Pathogens, Vol. 10, p. e1003888.
Oh, Sangmi Kwon, Bosung Kong, Sunju Yang, Gyongseon Lee, Nakyung Han, Dawoon Goo, Junghyun Siqueira-Neto, Jair L. Freitas-Junior, Lucio H. Liuzzi, Michel Lee, Jinhwa and Song, Rita 2014. Synthesis and biological evaluation of 2-acetamidothiophene-3-carboxamide derivatives against Leishmania donovani. Med. Chem. Commun., Vol. 5, p. 142.
Mittra, Bidyottam Cortez, Mauro Haydock, Andrew Ramasamy, Gowthaman Myler, Peter J. and Andrews, Norma W. 2013. Iron uptake controls the generation ofLeishmaniainfective forms through regulation of ROS levels. The Journal of Experimental Medicine, Vol. 210, p. 401.
Tsigankov, Polina Gherardini, Pier Federico Helmer-Citterich, Manuela Späth, Gerald F. and Zilberstein, Dan 2013. Phosphoproteomic Analysis of DifferentiatingLeishmaniaParasites Reveals a Unique Stage-Specific Phosphorylation Motif. Journal of Proteome Research, Vol. 12, p. 3405.
Gutiérrez-Kobeh, Laila de Oyarzabal, Eleané Argueta, Jesús Wilkins, Arturo Salaiza, Norma Fernández, Edith López, Oliver Aguirre, Magdalena and Becker, Ingeborg 2013. Inhibition of dendritic cell apoptosis by Leishmania mexicana amastigotes. Parasitology Research, Vol. 112, p. 1755.
Mittra, Bidyottam and Andrews, Norma W. 2013. IRONy OF FATE: role of iron-mediated ROS in Leishmania differentiation. Trends in Parasitology, Vol. 29, p. 489.
TSIGANKOV, POLINA GHERARDINI, PIER FEDERICO HELMER-CITTERICH, MANUELA and ZILBERSTEIN, DAN 2012. What has proteomics taught us about Leishmania development?. Parasitology, Vol. 139, p. 1146.
Castanys-Muñoz, E Brown, E Coombs, G H and Mottram, J C 2012. Leishmania mexicana metacaspase is a negative regulator of amastigote proliferation in mammalian cells. Cell Death and Disease, Vol. 3, p. e385.
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A new method is described which has made possible the long-term axenic cultivation of Leishmania mexicana amastigotelike forms in Schneider's Drosophila medium supplemented with 2% (v/v) foetal calf serum. Unlike previous methods, it utilizes direct culture of parasites obtained from the lesions of infected animals rather than adaptation of promastigotes in vitro. Ultrastructural (possession of megasomes), biochemical (cysteine proteinase activity and gelatin SDS-PAGE banding pattern) and infectivity (in vivo) data are presented which show the close similarity of the cultured forms to lesion amastigotes. The axenically cultured forms grew optimally at a temperature of 32–33 °C, providing further evidence for their amastigote nature. It was found that adjustment of the pH of the growth medium to 5·4 was required in order to retain the amastigote morphology of the cultured parasites. This supports the notion that leishmanial amastigotes are acidophiles.
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