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Catalase expression impairs oxidative stress-mediated signalling in Trypanosoma cruzi

Published online by Cambridge University Press:  27 June 2017

ANNA CLÁUDIA GUIMARÃES FREIRE ,
CERES LUCIANA ALVES ,
GRAZIELLE RIBEIRO GOES ,
BRUNO CARVALHO RESENDE ,
NILMAR SILVIO MORETTI ,
VINÍCIUS SANTANA NUNES ,
PEDRO HENRIQUE NASCIMENTO AGUIAR ,
ERICH BIRELLI TAHARA ,
GLÓRIA REGINA FRANCO  and
ANDRÉA MARA MACEDO
...Show all authors
ANNA CLÁUDIA GUIMARÃES FREIRE
Affiliation:
Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
CERES LUCIANA ALVES
Affiliation:
Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
GRAZIELLE RIBEIRO GOES
Affiliation:
Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
BRUNO CARVALHO RESENDE
Affiliation:
Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
NILMAR SILVIO MORETTI
Affiliation:
Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil
VINÍCIUS SANTANA NUNES
Affiliation:
Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil
PEDRO HENRIQUE NASCIMENTO AGUIAR
Affiliation:
Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
ERICH BIRELLI TAHARA
Affiliation:
Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
GLÓRIA REGINA FRANCO
Affiliation:
Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
ANDRÉA MARA MACEDO
Affiliation:
Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
SÉRGIO DANILO JUNHO PENA
Affiliation:
Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
FERNANDA RAMOS GADELHA
Affiliation:
Departamento de Bioquímica e Biologia Tecidual, Instituto de Biologia, UNICAMP, Campinas, SP, Brazil
ALESSANDRA APARECIDA GUARNERI
Affiliation:
Centro de Pesquisas René Rachou, FIOCRUZ, Belo Horizonte, MG, Brazil
SERGIO SCHENKMAN
Affiliation:
Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil
LEDA QUERCIA VIEIRA
Affiliation:
Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
CARLOS RENATO MACHADO*
Affiliation:
Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
*
*Corresponding author: Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. E-mail: crmachad@icb.ufmg.br
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Summary

Trypanosoma cruzi is exposed to oxidative stresses during its life cycle, and amongst the strategies employed by this parasite to deal with these situations sits a peculiar trypanothione-dependent antioxidant system. Remarkably, T. cruzi’s antioxidant repertoire does not include catalase. In an attempt to shed light on what are the reasons by which this parasite lacks this enzyme, a T. cruzi cell line stably expressing catalase showed an increased resistance to hydrogen peroxide (H2O2) when compared with wild-type cells. Interestingly, preconditioning carried out with low concentrations of H2O2 led untransfected parasites to be as much resistant to this oxidant as cells expressing catalase, but did not induce the same level of increased resistance in the latter ones. Also, presence of catalase decreased trypanothione reductase and increased superoxide dismutase levels in T. cruzi, resulting in higher levels of residual H2O2 after challenge with this oxidant. Although expression of catalase contributed to elevated proliferation rates of T. cruzi in Rhodnius prolixus, it failed to induce a significant increase of parasite virulence in mice. Altogether, these results indicate that the absence of a gene encoding catalase in T. cruzi has played an important role in allowing this parasite to develop a shrill capacity to sense and overcome oxidative stress.

Keywords

Trypanosoma cruzicatalaseoxidative stressoxidative signalling
Type
Research Article
Information
Parasitology , Volume 144 , Issue 11 , September 2017 , pp. 1498 - 1510
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Copyright
Copyright © Cambridge University Press 2017 

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