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Characterization of actin genes in Bonamia ostreae and their application to phylogeny of the Haplosporidia

Published online by Cambridge University Press:  03 August 2007

I. LÓPEZ-FLORES*
Affiliation:
Laboratoire de Génétique et Pathologie. Station La Tremblade, IFREMER. Avenue Mus de Loup, 17390 La Tremblade, France
V. N. SUÁREZ-SANTIAGO
Affiliation:
Department of Botany, Faculty of Sciences, University of Granada, Avenida Severo Ochoa s/n, 18071 Granada, Spain
D. LONGET
Affiliation:
Department of Zoology and Animal Biology, University of Geneva, Sciences III, 30 Quai Ernest Ansermet, CH 1211 Geneva 4, Switzerland
D. SAULNIER
Affiliation:
Laboratoire de Génétique et Pathologie. Station La Tremblade, IFREMER. Avenue Mus de Loup, 17390 La Tremblade, France
B. CHOLLET
Affiliation:
Laboratoire de Génétique et Pathologie. Station La Tremblade, IFREMER. Avenue Mus de Loup, 17390 La Tremblade, France
I. ARZUL
Affiliation:
Laboratoire de Génétique et Pathologie. Station La Tremblade, IFREMER. Avenue Mus de Loup, 17390 La Tremblade, France
*
*Corresponding author: Present address: Laboratoire de Génétique et Pathologie, IFREMER. Avenue Mus de Loup, 17390 La Tremblade, France. Tel: +33 546762647. Fax: +33 546762611. E-mail: ilopez@ugr.es

Summary

Bonamia ostreae is a protozoan parasite that infects the European flat oyster Ostrea edulis, causing systemic infections and resulting in massive mortalities in populations of this valuable bivalve species. In this work, we have characterized B. ostreae actin genes and used their sequences for a phylogenetic analysis. Design of different primer sets was necessary to amplify the central coding region of actin genes of B. ostreae. Characterization of the sequences and their amplification in different samples demonstrated the presence of 2 intragenomic actin genes in B. ostreae, without any intron. The phylogenetic analysis placed B. ostreae in a clade with Minchinia tapetis, Minchinia teredinis and Haplosporidium costale as its closest relatives, and demonstrated that the paralogous actin genes found in Bonamia resulted from a duplication of the original actin gene after the Bonamia origin.

Type
Research Article
Copyright
Copyright © Cambridge University Press 2007

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References

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