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Characterization of peptidases of adult Trichuris muris

  • L. J. Drake (a1), A. E. Bianco (a1), D. A. P. Bundy (a1) and F. Ashall (a1)
  • DOI:
  • Published online: 01 April 2009

Excretory/secretory (E/S) material of Trichuris muris was found to contain 2 major peptidases, Mr 85 and 105 kDa, which degrade gelatin optimally at pH 6·0 in sodium dodecyl sulphate–polyacrylamide gels. The peptidases were inactivated diisopropylfluorophosphate, leupeptin and soybean trypsin inhibitor, but were unaffected by inhibitors of aspartic-, cysteine- and metallo-peptidases, indicating that they are serine peptidases. Both enzymes were detectable within 5 h after incubation of worms in culture medium and showed a time-dependent increase in levels. Neither peptidase was detected in worm extracts, suggesting that they are activated during or following secretion from worms. Live worms degraded radio-isotope labelled extracellular matrix protein substratum derived from mammalian cells. Aminopeptidase activities capable of catalysing hydrolysis of amino acyl aminomethylcoumarin (MCA) substrates and a Z-Phe-Arg-MCA-hydrolysing cysteine peptidase activity, were detected in extracts of adult worms but not in E/S material.

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F. Ashall , H. Harris , N. Healy & E. Shaw (1990). Substrate specificity and inhibitor sensitivity of a trypanosomatid alkaline peptidase. Biochimica et Biophysica Acta 1035, 293–9.

M. M. Bradford (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Annals of Biochemistry 72, 248–54.

C. Braun-Breton , T. L. Rosenberry & L. P. Da Silva (1988). Induction of the proteolytic activity of a membrane protein in Plasmodium falciparum by phosphatidyl inositol-specific phospholipase C. Nature, London332, 457–9.

D. A. P. Bundy & E. S. Cooper (1989). Trichuris and trichuriasis in humans. Advances in Parasitology 28, 107–73.

C. L. Chappell & M. H. Dresden (1986). Schistosoma mansoni: protease activity of haemoglobinase from the digestive tract of adult worms. Experimental Parasitology 61, 160–7.

E. S. Cooper & D. A. P. Bundy (1988). Trichuris is not trivial. Parasitology Today 4, 301–6.

S. Greig & F. Ashall (1990). Electrophoretic characterisation of Trypanosoma cruzi peptidases. Molecular and Biochemical Parasitology 39, 31–8.

D. E. Hill , H. R. Gamble , M. L. Rhoads , R. H. Fetterer & J. F. Urban (1993). Trichuris suis: a zinc metalloprotease from culture fluids of adult parasites. Experimental Parasitology 77, 170–8.

R. M. Lewert & C. L. Lee (1954). Studies on the passage of helminth larvae through host tissues. I. Histochemical studies on the extracellular changes caused by penetrating larvae. II. Enzymatic activity of larvae in vivo and in vitro. Journal of Infectious Diseases 95, 1326.

M. Marikovsky , Z. Fishelson & R. Arnon (1988). Purification and characterisation of proteases secreted by transforming schistosomula of Schistosoma mansoni. Molecular and Biochemical Parasitology 30, 4554.

J. H. McKerrow , P. Jones , H. Sage & S. Pino-Heiss (1985). Proteinases of invasive larvae of the trematode parasite Schistosoma mansoni degrade connective tissue and basement membrane macromolecules. The Biochemical Journal 231, 4751.

J. H. McKerrow , P. Brindley , M. Brown , A. A. Gam , C. Staunton & F. A. Neva (1990). Strongyloides stercoralis: identification of a protease that facilitates penetration of skin by the infective larvae. Experimental Parasitology 70, 134–43.

R. H. Nimmo-Smith & J. E. D. Keeling (1960). Some hydrolytic enzymes of the parasitic nematode Trichuris muris. Experimental Parasitology 10, 337–55.

T. S. Panesar (1981). The early phase of tissue invasion by Trichuris muris (Nematoda: Trichuroidea). Zeitschrift für Parasitenkunde 66, 163–6.

J. L. Silen & D. A. Agard (1989). The α-lytic protease pro-region does not require a physical linkage to activate the protease domain in vivo. Nature, London341, 462–4.

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