Skip to main content Accessibility help
×
Home

Characterization of the lipid content of Toxoplasma gondii rhoptries

  • F. Foussard (a1), M. A. Leriche (a2) and J. F. Dubremetz (a2)

Extract

Quantitative and qualitative analysis of lipids has been performed on a rhoptry fraction purified from Toxoplasma gondii tachyzoites. The lipid to protein ratio was estimated to be 0·26. The cholesterol to phospholipid ratio was unusually high at 1·48. Phosphatidylcholine was the major phospholipid; phosphatidylserine, phosphatidylinositol and sphingomyelin were absent whereas significant amounts of phosphatidic acid and lysophospholipids were found. This pelicular composition could be related to functional involvement of the organelle in host-cell invasion.

Copyright

References

Hide All
Bannister, L. H. & Mitchell, G. H. (1989). The fine structure of secretion by Plasmodium knowlesi merozoites during red cell invasion. Journal of Protozoology 36, 362–7.
Bottcher, C. S. F., Van Gent, C. M. & Pries, C. (1961). A rapid and sensitive sub-microphosphorous determination. Analytical and Clinical Acta 24, 203–4.
Gallois, Y., Foussard, F., Girault, A., Hodbert, J., Tricaud, A., Mauras, G. & Motta, C. (1988). Membrane fluidity of Toxoplasma gondii: a fluorescence polarization study. Biology of the Cell 62, 1115.
Grimwood, B. G., Hechemy, K. & Stevens, R. W. (1979). Toxoplasma gondii: Purification of trophozoites propagated in cell culture. Experimental Parasitology 48, 282–6.
Kolarovic, L. & Fournier, N. C. (1986). A comparison of extraction methods for the isolation of phospholipids from biological sources. Analytical Biochemistry 156, 244–50.
Le, A. V. & Doyle, D. L. (1984). General theory of membrane structure and function. In Membranes, Detergents and Receptor Solubilization, pp. 125. New York: Alan R. Liss Inc.
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193, 256–75.
Mikkelsen, R. B., Kamber, M., Wadwa, K. S., Lin, P. S. & Schmidt-Ullrich, R. (1988). The role of lipids in Plasmodium falciparum invasion of erythrocytes: a coordinated biochemical and microscopic analysis. Proceedings of the National Academy of Sciences, USA 85, 5956–60.
Sabin, A. B. (1941). Toxoplasmic encephalitis in children. Journal of the American Medical Association 116, 801–7.
Sadak, A., Taghy, Z., Fortier, B. & Dubremetz, J. F. (1988). Characterization of a family of rhoptry proteins of Toxoplasma gondii. Molecular and Biochemical Parasitology 29, 203–12.
Saffer, L. D., Long-Krug, S. A. & Schwartzman, J. D. (1989). The role of phospholipase in host cell penetration by Toxoplasma gondii. American Journal of Tropical Medicine and Hygiene 40, 145–9.
Schwartzman, J. D. & Krug, E. C. (1989). Toxoplasma gondii: characterization of monoclonal antibodies that recognize rhoptries. Experimental Parasitology 68, 7482.
Spector, A. A. & Yorek, M. A. (1985). Membrane lipid composition and cellular function. Journal of Lipid Research 26, 1015–35.

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed