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Cholinergic inhibition of muscle fibres isolated from Schistosoma mansoni (Trematoda: Digenea)

Published online by Cambridge University Press:  06 April 2009

T. A. Day ,
G-Z. Chen ,
C. Miller ,
M. Tian ,
J. L. Bennett  and
R. A. Pax
T. A. Day
Affiliation:
Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48824, USA
G-Z. Chen
Affiliation:
Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48824, USA
C. Miller
Affiliation:
Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48824, USA
M. Tian
Affiliation:
Department of Zoology, Michigan State University, East Lansing, MI 48824, USA
J. L. Bennett
Affiliation:
Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48824, USA
R. A. Pax*
Affiliation:
Department of Zoology, Michigan State University, East Lansing, MI 48824, USA
*
*Corresponding author. Tel: 517-353-5447. Fax: 517-432-2789. Email: pax@pilot.msu.edu
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Summary

Cholinergic compounds inhibit FMRFamide-induced contractions in dispersed muscle fibres isolated from adult Schistosoma mansoni. Acetylcholine (ACh) was the most effective cholinergic agonist tested with an EC50 < 100 nM. Less effective were propionylcholine and arecoline with EC50 < 1 μM and butyrylcholine and carbachol with EC50 < 10μM. Choline, muscarine, pilocarpine, nicotine, DMPP (1,1-dimethylphenylpiperazine ) and levamisole were all ineffective. Amongst tested antagonists, d-tubocurarine (100μM), mecamylamine (1 mM), atropine (1 mM), scopolamine (1 mM) and quinuclidinyl benzilate (10 μM) were all ineffective. Bicuculline, picrotoxin and strychnine were also ineffective. However α-bungarotoxin, at 100 nM, was able to block the inhibitory ACh effect. From these data it appears that the cholinergic receptor on the schistosome muscle fibres may be of the nicotinic type, but that its pharmacology is different from that of nicotinic receptors of vertebrates as well as of nematodes or a variety of other invertebrates.

Keywords

PlatyhelminthesTrematodaSchistosoma mansonimuscle fibresacetylcholinecholinergic receptorscholinergic agonistscholinergic antagonists
Type
Research Article
Information
Parasitology , Volume 113 , Issue 1 , July 1996 , pp. 55 - 61
Copyright
Copyright © Cambridge University Press 1996

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