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Cytochemical localization of cytochrome oxidase in tissues of parasitic nematodes

  • M. Fry (a1) and J. E. Beesley (a2)
Extract

Cytochemical staining with diaminobenzidine (DAB) has been used to locate cytochrome oxidase activity in mitochondria from major tissues of three intestinal parasitic nematodes, Nippostrongylus brasiliensis, Haemonchus contortus and Ascaridia galli. Deposition of DAB reaction product was uniform and relatively intense in mitochondria from all tissues studied in N. brasiliensis. However, mitochondria from different tissues of H. contortus and A. galli varied considerably in their cytochemical staining, decreasing in the order hypodermis < muscle < gut < reproductive tissue. Mitochondria in gut and reproductive tissue of A. galli possessed little or no cytochrome oxidase activity. These results are compatible with respect to oxygen availability and the extent and localization of the mammalian-type aerobic mitochondrial electron transport pathway in intestinal parasitic nematodes.

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COPYRIGHT: © Cambridge University Press 1985
References
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Atkinson, H. J. (1980). Respiration in nematodes. In Nematodes as Biological Models, vol. 2 (ed. Zuckerman, B. M.), pp. 101–42. New York and London: Academic Press.
Barrett, J. (1981). Biochemistry of Parasitic Helminths, pp. 72–148. London: MacMillan Publishers Limited.
Bryant, C. (1970). Electron transport in parasitic helminths and protozoa. Advances in Parasitology 8, 139–72.
Fry, M., Bazil, C. & Jenkins, D. C. (1983). A comparison of mitochondrial electron transport in the intestinal parasitic nematodes Nippostrongylus brasiliensis and Ascaridia galli. Comparative Biochemistry and Physiology 75B, 451–60.
Fry, M. & Brazeley, E. P. (1984). NADH-fumarate reductase and succinate dehydrogenase activities in mitochondria of Ascaridia galli and Nippostrongylus brasiliensis. Comparative Biochemistry and Physiology 77B, 143–50.
Fry, M. & Jenkins, D. C. (1983). Nippostrongylus brasiliensis and Ascaridia galli: Mitochondrial respiration in free-living and parasitic stages. Experimental Parasitology 56, 101–6.
Fry, M. & Jenkins, D. C. (1984 a). Nematoda: Aerobic respiratory pathways of adult parasitic species. Experimental Parasitology 57, 86–92.
Fry, M. & Jenkins, D. C. (1984 b). Nippostrongylus brasiliensis: the effect of mitochondrial inhibitors on life-cycle stages. Parasitology 88, 163–77.
Lloyd, D., Mellor, H. & Williams, J. L. (1983). Oxygen affinity of the respiratory chain of Acanthamoeba castellanii. The Biochemical Journal 214, 47–51.
Rodrick, G. E., Long, S. D., Sodeman, W. A. Jr. & Smith, D. L. (1982). Ascaris suum: Oxidative phosphorylation in mitochondria from developing eggs and adult muscle. Experimental Parasitology 54, 235–42.
Seligman, A. M., Karnovsky, M. J., Wasserkrug, H. L. & Hanker, J. S. (1968). Nondroplet ultrastructural demonstration of cytochrome oxidase activity with a polymerising osmiophilic reagent, diaminobenzidine (DAB). Journal of Cell Biology 38, 1–14.
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Parasitology
  • ISSN: 0031-1820
  • EISSN: 1469-8161
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