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Different protein of Echinococcus granulosus stimulates dendritic induced immune response

Published online by Cambridge University Press:  25 February 2015

YANA WANG ,
QIANG WANG ,
SHIYU LV  and
SHENGXIANG ZHANG
YANA WANG
Affiliation:
Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, School of Life Sciences, Lanzhou University, 222 South Tianshui Road, Lanzhou, Gansu 730000, China Echinococcosis Laboratory, Ningxia Medical University, Yinchun 750004, China
QIANG WANG
Affiliation:
Echinococcosis Laboratory, Ningxia Medical University, Yinchun 750004, China
SHIYU LV
Affiliation:
School of Laboratory Medicine, Ningxia Medical University, Yinchuan 750004, China
SHENGXIANG ZHANG*
Affiliation:
Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, School of Life Sciences, Lanzhou University, 222 South Tianshui Road, Lanzhou, Gansu 730000, China
*
*Corresponding author. School of Life Sciences, Lanzhou University, 222 South Tianshui Road, Lanzhou, Gansu 730000, China. E-mail: sxzhang@lzu.edu.cn
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Summary

Cystic echinococcosis is a chronic infectious disease that results from a host/parasite interaction. Vaccination with ferritin derived from Echinococcus granulosus is a potential preventative treatment. To understand whether ferritin is capable of inducing a host immune response, we investigated the response of dendritic cells (DCs) to both recombinant ferritin protein and the hydatid fluid (HF) of E. granulosus. We evaluated the immunomodulatory potential of these antigens by performing, immunocytochemistry, electron microscopy and in vivo imaging of monocyte-derived murine DCs. During antigen stimulation of DCs, ferritin cause DCs maturation and induced higher levels of surface marker expression and activated T-cell proliferation and migration. On contrary, HF failed to induce surface marker expression and to stimulate T-cell proliferation. In response to HF, DCs produced interleukin-6 (IL-6), but no IL-12 and IL-10. DCs stimulated with ferritin produced high levels of cytokines. Overall, HF appears to induce host immunosuppression in order to ensure parasite survival via inhibits DC maturation and promotes Th2-dependent secretion of cytokines. Although ferritin also promoted DC maturation and cytokine release, it also activates CD4+T-cell proliferation, but regard of the mechanism of the Eg.ferritin induce host to eradicate E. granulosus were not clear.

Keywords

dendritic cellrecombinant ferritinEchinococcus granulosushydatid fluidimmune response
Type
Research Article
Information
Parasitology , Volume 142 , Issue 7 , June 2015 , pp. 879 - 889
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Copyright
Copyright © Cambridge University Press 2015 

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